iPSC-derived type IV collagen α5-expressing kidney organoids model Alport syndrome

ヒトiPS細胞から作製した腎オルガノイドを用いたアルポート症候群病態モデルの開発. 京都大学プレスリリース. 2023-09-28.iPSC-derived kidney organoids to model a lifelong renal disease. 京都大学プレスリリース. 2023-10/17.Alport syndrome (AS) is a hereditary glomerulonephritis caused by COL4A3, COL4A4 or COL4A5 gene mutations and characterized by abnormalities of glomerular basement membranes (GBMs). Due to a lack of curative treatments, the condition proceeds to end-stage renal disease even in adolescents. Hampering drug discovery is the absence of effective in vitro methods for testing the restoration of normal GBMs. Here, we aimed to develop kidney organoid models from AS patient iPSCs for this purpose. We established iPSC-derived collagen α5(IV)-expressing kidney organoids and confirmed that kidney organoids from COL4A5 mutation-corrected iPSCs restore collagen α5(IV) protein expression. Importantly, our model recapitulates the differences in collagen composition between iPSC-derived kidney organoids from mild and severe AS cases. Furthermore, we demonstrate that a chemical chaperone, 4-phenyl butyric acid, has the potential to correct GBM abnormalities in kidney organoids showing mild AS phenotypes. This iPSC-derived kidney organoid model will contribute to drug discovery for AS

霊長類におけるMHCミスマッチ移植におけるiPSC由来網膜の移植適応性

京都大学新制・課程博士博士(医学)甲第24792号医博第4984号新制||医||1066(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 椛島 健治, 教授 辻川 明孝, 教授 山中 伸弥学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

地域イノベーションエコシステムの実証研究

兵庫県立大学大学院201

LAUREATES: Award-Winning Scholars at Kyoto University

Page 04: President's MessagePage 05: IntroductionPage 07: About Kyoto UniversityPage 08: [Chapter 1] Nobel PrizePage 17: AWARD HISTORY 1949-1977Page 18: [Chapter 2] Gauss Prize, Wolf Prize, Fields Medal, Albert Lasker AwardPage 26: [Chapter 3] Kyoto Prize, Japan Prize, Honda PrizePage 33: AWARD HISTORY 1978-1995Page 34: [Chapter 4] International Awards in Science and EngineeringPage 51: AWARD HISTORY 1996-2007Page 52: [Chapter 5] International Awards in Medicine and Life SciencePage 66: [Chapter 6] International Awards in Humanities and Social SciencePage 72: [Chapter 7] Order of Culture, JapanPage 82: [Chapter 8] Imperial Prize of the Japan Academ

Submerged Robotic Micromanipulation and Dielectrophoretic Micro-objet release.

International audienceThe development of new hybrid microsystems needs new technologies which are able to perform assembly of small micro-objects. Now, the current micromanipulation technologies are still unreliable for micro-objects which typical size is down to hundred micrometers. Consequently, the study and the development of innovative artificial microobject manipulation strategies in these dimensions is particularly relevant. As presented in the literature, micromanipulations are perturbed by the adhesion and surface forces which depend on surrounding mediums. We propose to perform micro-assembly tasks in liquid medium, because adhesion and surface forces applied on submerged micro-objects are less important than in air. The comparative analysis of micro-forces in air and in liquid is presented in this paper. Although the micro-forces reduce in liquid, they stay disturbed the micro-objects release. Thus, we propose to extend the dielectrophoresis micromanipulation principles which are currently done in the biological micromanipulation to submerged artificial objects micro-assembly. The negative dielectrophoresis principle is used to release a micro-object grasped with a micro-gripper. Physical principle and first experimentations is presented in this article. Further works will focus on the optimization of the principle, and on the micro-object release modelling and control

Longitudinal diary data: Six months real-world implementation of affordable companion robots for older people in supported living

Companion robots have potential for improving wellbeing within aged care, however literature focuses on shorter-term studies often using relatively expensive platforms, raising concerns around novelty effects and economic viability. Here, we report ecologically valid diary data from two supported living facilities for older people with dementia or learning difficulties. Both sites implemented Joy for All robot animals and maintained diaries for six months. Entries were analysed using thematic analysis. We found robot use increased over the six months, changing from short, structured sessions to mainly permanent availability. Thus previously reported concerns on novelty were not warranted. Both sites reported positive outcomes including reminiscence, improved communication and potential wellbeing benefits (reduced agitation/anxiety). Incidences of negative responses included devices described as 'creepy.' Devices appeared sufficiently robust for prolonged daily use with multiple users. Overall, we provide insight into real-world implementation of affordable companion robots, and longitudinal development of use

Longitudinal Diary Data: Six Months Real-world Implementation of Affordable Companion Robots for Older People in Supported Living

Companion robots have potential for improving wellbeing within aged care, however literature focuses on shorter-term studies often using relatively expensive platforms, raising concerns around novelty effects and economic viability. Here, we report ecologically valid diary data from two supported living facilities for older people with dementia or learning difficulties. Both sites implemented Joy for All robot animals and maintained diaries for six months. Entries were analysed using thematic analysis. We found robot use increased over the six months, changing from short, structured sessions to mainly permanent availability. Thus previously reported concerns on novelty were not warranted. Both sites reported positive outcomes including reminiscence, improved communication and potential wellbeing benefits (reduced agitation/anxiety). Incidences of negative response included devices described as ‘creepy.’ Devices appeared sufficiently robust for prolonged daily use with multiple users. Overall, we provide insight into real-world implementation of affordable companion robots, and longitudinal development of use