Hyperspectral colon tissue cell classification

A novel algorithm to discriminate between normal and malignant tissue cells of the human colon is presented. The microscopic level images of human colon tissue cells were acquired using hyperspectral imaging technology at contiguous wavelength intervals of visible light. While hyperspectral imagery data provides a wealth of information, its large size normally means high computational processing complexity. Several methods exist to avoid the so-called curse of dimensionality and hence reduce the computational complexity. In this study, we experimented with Principal Component Analysis (PCA) and two modifications of Independent Component Analysis (ICA). In the first stage of the algorithm, the extracted components are used to separate four constituent parts of the colon tissue: nuclei, cytoplasm, lamina propria, and lumen. The segmentation is performed in an unsupervised fashion using the nearest centroid clustering algorithm. The segmented image is further used, in the second stage of the classification algorithm, to exploit the spatial relationship between the labeled constituent parts. Experimental results using supervised Support Vector Machines (SVM) classification based on multiscale morphological features reveal the discrimination between normal and malignant tissue cells with a reasonable degree of accuracy

Improved Feature Extraction, Feature Selection, and Identification Techniques That Create a Fast Unsupervised Hyperspectral Target Detection Algorithm

This research extends the emerging field of hyperspectral image (HSI) target detectors that assume a global linear mixture model (LMM) of HSI and employ independent component analysis (ICA) to unmix HSI images. Via new techniques to fully automate feature extraction, feature selection, and target pixel identification, an autonomous global anomaly detector, AutoGAD, has been developed for potential employment in an operational environment for real-time processing of HSI targets. For dimensionality reduction (initial feature extraction prior to ICA), a geometric solution that effectively approximates the number of distinct spectral signals is presented. The solution is based on the theory of the shape of the eigenvalue curve of the covariance matrix of spectral data containing noise. For feature selection, previously a subjective definition called significant kurtosis change was used to denote the separation between targets classes and non-target classes. This research presents two new measures, potential target signal to noise ratio (PT SNR) and max pixel score which computed for each of the ICA features to create a new two dimensional feature space where the overlap between target and non-target classes is reduced compared to the one dimensional kurtosis value feature space. Finally, after target feature selection, adaptive noise filtering, but with an iterative approach, is applied to the signals. The effect is a reduction in the power of the noise while preserving the power of the target signal prior to target identification to reduce false positive detections. A zero-detection histogram method is applied to the smoothed signals to identify target locations to the user. MATLAB code for the AutoGAD algorithm is provided

Wavelet based segmentation of hyperspectral colon tissue imagery

Segmentation is an early stage for the automated classification of tissue cells between normal and malignant types. We present an algorithm for unsupervised segmentation of images of hyperspectral human colon tissue cells into their constituent parts by exploiting the spatial relationship between these constituent parts. This is done by employing a modification of the conventional wavelet based texture analysis, on the projection of hyperspectral image data in the first principal component direction. Results show that our algorithm is comparable to other more computationally intensive methods which exploit the spectral characteristics of the hyperspectral imagery data

Unsupervised spectral sub-feature learning for hyperspectral image classification

Spectral pixel classification is one of the principal techniques used in hyperspectral image (HSI) analysis. In this article, we propose an unsupervised feature learning method for classification of hyperspectral images. The proposed method learns a dictionary of sub-feature basis representations from the spectral domain, which allows effective use of the correlated spectral data. The learned dictionary is then used in encoding convolutional samples from the hyperspectral input pixels to an expanded but sparse feature space. Expanded hyperspectral feature representations enable linear separation between object classes present in an image. To evaluate the proposed method, we performed experiments on several commonly used HSI data sets acquired at different locations and by different sensors. Our experimental results show that the proposed method outperforms other pixel-wise classification methods that make use of unsupervised feature extraction approaches. Additionally, even though our approach does not use any prior knowledge, or labelled training data to learn features, it yields either advantageous, or comparable, results in terms of classification accuracy with respect to recent semi-supervised methods