High-speed in vitro intensity diffraction tomography

We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume three-dimensional (3-D) refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume rates. Using eight intensity images, we recover volumes of ∼350 μm  ×  100 μm  ×  20  μm, with near diffraction-limited lateral resolution of   ∼  487  nm and axial resolution of   ∼  3.4  μm. The attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length scales. We demonstrate aIDT’s capabilities on unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens. Within these samples, we recover macroscale cellular structures, subcellular organelles, and dynamic micro-organism tissues with minimal motion artifacts. Quantifying such features has significant utility in oncology, immunology, and cellular pathophysiology, where these morphological features are evaluated for changes in the presence of disease, parasites, and new drug treatments. Finally, we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed technique. aIDT shows promise as a powerful high-speed, label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.https://arxiv.org/abs/1904.06004Accepted manuscrip

Multi-Scale 3D Scene Flow from Binocular Stereo Sequences

Scene flow methods estimate the three-dimensional motion field for points in the world, using multi-camera video data. Such methods combine multi-view reconstruction with motion estimation. This paper describes an alternative formulation for dense scene flow estimation that provides reliable results using only two cameras by fusing stereo and optical flow estimation into a single coherent framework. Internally, the proposed algorithm generates probability distributions for optical flow and disparity. Taking into account the uncertainty in the intermediate stages allows for more reliable estimation of the 3D scene flow than previous methods allow. To handle the aperture problems inherent in the estimation of optical flow and disparity, a multi-scale method along with a novel region-based technique is used within a regularized solution. This combined approach both preserves discontinuities and prevents over-regularization – two problems commonly associated with the basic multi-scale approaches. Experiments with synthetic and real test data demonstrate the strength of the proposed approach.National Science Foundation (CNS-0202067, IIS-0208876); Office of Naval Research (N00014-03-1-0108

A bayesian approach to simultaneously recover camera pose and non-rigid shape from monocular images

© . This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/In this paper we bring the tools of the Simultaneous Localization and Map Building (SLAM) problem from a rigid to a deformable domain and use them to simultaneously recover the 3D shape of non-rigid surfaces and the sequence of poses of a moving camera. Under the assumption that the surface shape may be represented as a weighted sum of deformation modes, we show that the problem of estimating the modal weights along with the camera poses, can be probabilistically formulated as a maximum a posteriori estimate and solved using an iterative least squares optimization. In addition, the probabilistic formulation we propose is very general and allows introducing different constraints without requiring any extra complexity. As a proof of concept, we show that local inextensibility constraints that prevent the surface from stretching can be easily integrated. An extensive evaluation on synthetic and real data, demonstrates that our method has several advantages over current non-rigid shape from motion approaches. In particular, we show that our solution is robust to large amounts of noise and outliers and that it does not need to track points over the whole sequence nor to use an initialization close from the ground truth.Peer ReviewedPostprint (author's final draft

Structure from Articulated Motion: Accurate and Stable Monocular 3D Reconstruction without Training Data

Recovery of articulated 3D structure from 2D observations is a challenging computer vision problem with many applications. Current learning-based approaches achieve state-of-the-art accuracy on public benchmarks but are restricted to specific types of objects and motions covered by the training datasets. Model-based approaches do not rely on training data but show lower accuracy on these datasets. In this paper, we introduce a model-based method called Structure from Articulated Motion (SfAM), which can recover multiple object and motion types without training on extensive data collections. At the same time, it performs on par with learning-based state-of-the-art approaches on public benchmarks and outperforms previous non-rigid structure from motion (NRSfM) methods. SfAM is built upon a general-purpose NRSfM technique while integrating a soft spatio-temporal constraint on the bone lengths. We use alternating optimization strategy to recover optimal geometry (i.e., bone proportions) together with 3D joint positions by enforcing the bone lengths consistency over a series of frames. SfAM is highly robust to noisy 2D annotations, generalizes to arbitrary objects and does not rely on training data, which is shown in extensive experiments on public benchmarks and real video sequences. We believe that it brings a new perspective on the domain of monocular 3D recovery of articulated structures, including human motion capture.Comment: 21 pages, 8 figures, 2 table