23,019 research outputs found
3D correlative single-cell imaging utilizing fluorescence and refractive index tomography
Cells alter the path of light, a fact that leads to well-known aberrations in
single cell or tissue imaging. Optical diffraction tomography (ODT) measures
the biophysical property that causes these aberrations, the refractive index
(RI). ODT is complementary to fluorescence imaging and does not require any
markers. The present study introduces RI and fluorescence tomography with
optofluidic rotation (RAFTOR) of suspended cells, quantifying the intracellular
RI distribution and colocalizing it with fluorescence in 3D. The technique is
validated with cell phantoms and used to confirm a lower nuclear RI for HL60
cells. Furthermore, the nuclear inversion of adult mouse photoreceptor cells is
observed in the RI distribution. The applications shown confirm predictions of
previous studies and illustrate the potential of RAFTOR to improve our
understanding of cells and tissues.Comment: 15 pages, 5 figure
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Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.
During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow
ROAM: a Rich Object Appearance Model with Application to Rotoscoping
Rotoscoping, the detailed delineation of scene elements through a video shot,
is a painstaking task of tremendous importance in professional post-production
pipelines. While pixel-wise segmentation techniques can help for this task,
professional rotoscoping tools rely on parametric curves that offer the artists
a much better interactive control on the definition, editing and manipulation
of the segments of interest. Sticking to this prevalent rotoscoping paradigm,
we propose a novel framework to capture and track the visual aspect of an
arbitrary object in a scene, given a first closed outline of this object. This
model combines a collection of local foreground/background appearance models
spread along the outline, a global appearance model of the enclosed object and
a set of distinctive foreground landmarks. The structure of this rich
appearance model allows simple initialization, efficient iterative optimization
with exact minimization at each step, and on-line adaptation in videos. We
demonstrate qualitatively and quantitatively the merit of this framework
through comparisons with tools based on either dynamic segmentation with a
closed curve or pixel-wise binary labelling
Text Line Segmentation of Historical Documents: a Survey
There is a huge amount of historical documents in libraries and in various
National Archives that have not been exploited electronically. Although
automatic reading of complete pages remains, in most cases, a long-term
objective, tasks such as word spotting, text/image alignment, authentication
and extraction of specific fields are in use today. For all these tasks, a
major step is document segmentation into text lines. Because of the low quality
and the complexity of these documents (background noise, artifacts due to
aging, interfering lines),automatic text line segmentation remains an open
research field. The objective of this paper is to present a survey of existing
methods, developed during the last decade, and dedicated to documents of
historical interest.Comment: 25 pages, submitted version, To appear in International Journal on
Document Analysis and Recognition, On line version available at
http://www.springerlink.com/content/k2813176280456k3
An Approach to a Comprehensive Test Framework for Analysis and Evaluation of Text Line Segmentation Algorithms
The paper introduces a testing framework for the evaluation and validation of text line segmentation algorithms. Text line segmentation represents the key action for correct optical character recognition. Many of the tests for the evaluation of text line segmentation algorithms deal with text databases as reference templates. Because of the mismatch, the reliable testing framework is required. Hence, a new approach to a comprehensive experimental framework for the evaluation of text line segmentation algorithms is proposed. It consists of synthetic multi-like text samples and real handwritten text as well. Although the tests are mutually independent, the results are cross-linked. The proposed method can be used for different types of scripts and languages. Furthermore, two different procedures for the evaluation of algorithm efficiency based on the obtained error type classification are proposed. The first is based on the segmentation line error description, while the second one incorporates well-known signal detection theory. Each of them has different capabilities and convenience, but they can be used as supplements to make the evaluation process efficient. Overall the proposed procedure based on the segmentation line error description has some advantages, characterized by five measures that describe measurement procedures
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