8,199 research outputs found

    Complicated objects: artifacts from the Yuanming Yuan in Victorian Britain

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    The 1860 spoliation of the Summer Palace at the close of the Second Opium War by British and French troops was a watershed event within the development of Britain as an imperialist nation, which guaranteed a market for opium produced in its colony India and demonstrated the power of its armed forces. The distribution of the spoils to officers and diplomatic corps by campaign leaders in Beijing was also a sign of the British Army’s rising power as an instrument of the imperialist state. These conditions would suggest that objects looted from the site would be integrated into an imperialist aesthetic that reflected and promoted the material benefits of military engagement overseas and foregrounded the circumstances of their removal to Britain for campaign members and the British public. This study mines sources dating to the two decades following the war – including British newspapers, auction house records, exhibition catalogs and works of art – to test this hypothesis. Findings show that initial movements of looted objects through the military and diplomatic corps did reinforce notions of imperialist power by enabling campaign members to profit from the spoliation through sales of looted objects and trophy displays. However, material from the Summer Palace arrived at a moment when British manufacturers and cultural leaders were engaged in a national effort to improve the quality of British goods to compete in the international marketplace and looted art was quickly interpolated in this national conversation. Ironically, the same “free trade” imperatives that motivated the invasion energized a new design movement that embraced Chinese ornament. As a consequence, political interpretations of the material outside of military collections were quickly joined by a strong response to Chinese ornament from cultural institutions and design leaders. Art from the Summer Palace held a prominent place at industrial art exhibitions of the postwar period and inspired new designs in a number of mediums. While the availability of Chinese imperial art was the consequence of a military invasion and therefore a product of imperialist expansion, evidence presented here shows that the design response to looted objects was not circumscribed by this political reality. Chinese ornament on imperial wares was ultimately celebrated for its formal qualities and acknowledged links to the Summer Palace were an indicator of good design, not a celebration of victory over a failed Chinese state. Therefore, the looting of the Summer Palace was ultimately an essential factor in the development of modern design, the essence of which is a break with Classical ornament

    Measurement of the Environmental Impact of Materials

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    Throughout their life cycles—from production, usage, through to disposal—materials and products interact with the environment (water, soil, and air). At the same time, they are exposed to environmental influences and, through their emissions, have an impact on the environment, people, and health. Accelerated experimental testing processes can be used to predict the long-term environmental consequences of innovative products before these actually enter the environment. We are living in a material world. Building materials, geosynthetics, wooden toys, soil, nanomaterials, composites, wastes and more are research subjects examined by the authors of this book. The interactions of materials with the environment are manifold. Therefore, it is important to assess the environmental impact of these interactions. Some answers to how this task can be achieved are given in this Special Issue

    Development of a Mass Spectrometric Method based on Hydrogen-Deuterium Exchange (HDX-MS) for the Determination of Epitope Regions of Glycoproteins in High Throughput

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    The vast majority of biological processes such as cell-cell communication, immune response, reaction catalysis and the protein transport are driven by protein-protein interactions (PPIs). Elucidation of PPIs, which when altered are associated with various pathophysiological processes, opened an emerging era of protein analysis. The knowledge of the PPIs interfaces is crucial to obtain deeper insights into the PPIs mode of action and how to modulate them. In particular, PPIs between antibodies and antigens are of great interest for diagnostic and therapeutic applications in the biopharmaceutical industry and scientific research. In addition to high-resolution techniques such as nuclear magnetic resonance spectroscopy (NMR), X-ray crystallography and cryo-electron microscopy (cryo-EM), hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has evolved to a powerful analysis method for the elucidation of PPI interfaces. Without introducing structural modifications, HDX-MS can be used to elucidate protein dynamics and protein-ligand interaction sites of proteins of almost unlimited size, with high tolerance against impurities, low sample consumption and reasonable resolution and throughput. In addition, HDX data reflect the native in-solution protein conformation. However, HDX-MS remains challenging for the analysis of proteins encompassing multiple post-translational modifications such as disulphide bonds and N-glycosylations. In the context of the present work, a setup and workflow for HDX-MS bottom-up analyses was established. A prerequisite for reliable and efficient HDX analyses is the precise control of the pH, temperature and timing, which was facilitated by a tailor made Semi-Automated Interface for HD exchange (SAIDE). The SAIDE enables flexible use of a HPLC-MS instrumentation for various analyses, efficient use of the laboratory space while displaying a cost efficient approach compared to fully automated, commercial HDX systems. With this setup, a protocol for HDX analyses was developed and optimised, which addressed critical generic HDX parameters such as efficient proteolysis and good reproducibility while keeping the back exchange as low as possible. Compared to dissolved pepsin, the proteolysis efficiency could be increased using pepsin immobilized on beads. This enabled a tenfold reduction in digestion time while achieving a higher number of peptides, run-to-run recovery, a lower HDX variance, and lower average peptide length. These optimisations were performed as part of an HDX study designed to elucidate the binding region of a clinical mAb drug candidate targeting Annexin-A1 in a calcium dependent manner. Subsequently, the HDX-MS protocol was improved in terms of sample throughput and adapted to another protein target, the receptor-binding domain (RBD) of SARS-CoV-2. The high reproducibility of the established protocol enabled its application in an HDX screening workflow using a lower number of deuteration time points. Epitopes of seven nanobodies were characterized within a time period of roughly four weeks. The HDX-MS screening approach was used to support evidence based identification of two lead candidates potent in their viral neutralization. Subsequent endeavours aimed to adapt the established screening approach to proteins encompassing multiple post-translational modification (PTMs). While extracellular proteins display attractive drug targets or are themselves used as biopharmaceuticals, nearly each of them encompass multiple PTMs such as N-glycosylated and disulphide bonds. Originated in their heterogeneity, N-glycosylations remain challenging for structural analysis such as HDX-MS. Here, a novel peptide N-glycanase from Rudaea cellulosilytica (Rc) was characterized that exhibits broad substrate specificity and high activity for deglycosylation of natively folded proteins. Thus, the enzyme was used to facilitate MS based top-down protein analytics and offers the opportunity for N-deglycosylation of peptides in several minutes. Moreover, following heterologous expression the enzyme can be obtained from E. coli with high yield sufficient purity. Due to its acidic pH optimum, the PNGase Rc was successfully used under challenging HDX-MS quenching conditions (0 °C; pH 2.5) in presence of commonly applied concentrations of reducing and denaturing agents Tris(2-carboxyethyl)phosphine (TCEP), urea and guanidinium chloride (GdmCl). As a proof-of-principle the PNGase Rc was integrated into the established HDX epitope screening workflow (post-proteolysis) resulting in the elucidation of four nanobody epitopes targeting the multiple N-glycosylated extracellular domain of the signal-regulatory protein alpha (SIRPα). The additional deglycosylation increased sequence coverage and redundancy and also enabled the detection of epitopes in proximity of N-glycosylation sites

    Numerical Simulations of Dusty Colliding Wind Binaries

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    Colliding Wind Binary (CWB) systems are relatively rare phenomena, but have a significant influence on galactic evolution in terms of dust production -- especially in the early universe. The mechanisms behind this dust production, however, are poorly understood. The strong winds from both partners in the binary system drive shocks that heat the dust forming region to temperatures in excess of 100 million Kelvin; whilst this region does rapidly cool, the initial shock temperatures would destroy any dust grains that formed outside the collision region. Furthermore, this collision region is difficult to observe and simulate, limiting our understanding of how grains form and evolve in this region. This thesis attempts to improve our understanding of the evolution of dust grains within these systems, particularly growth of these grains from small dust grain cores to micron-scale grains. A co-moving dust grain model was implemented that simulates growth through accretion of gas onto the dust grains, as well as destruction through gas-grain sputtering. The model also simulates cooling through collisional excitation and subsequent emission for both dust grains and gas. Overall, the goal of this model was to determine how dust growth was influenced by the wind and orbital characteristics of the system, and which of these characteristics were most important for dust growth. First, a parameter space exploration of dust producing CWB systems (WCd systems) was conducted, varying the orbital separation, the wind terminal velocity and the mass loss rate of each star. It was found that dust production is strongly influenced by the ratio of wind terminal velocities between each star, as well as the orbital separation. Following up on this, a limited simulation of the episodic dust forming system WR140 was conducted, in order to understand how variance in orbital separation through eccentricity changed dust production rates over the course of a periastron passage. Furthermore, it was determined that dust production occurs over a very short period immediately prior to periastron passage and a small period after, with an ``active'' phase of approximately 1 year, or an eighth of the systems orbital period Whilst there is much to be done in the future, and many more systems to be simulated (in particular the recently discovered WR+WR CWB systems WR48a and WR70-16) this model is a good first step towards shedding light on these elusive and dust-shrouded systems

    Developing active biomaterials for implantable devices: platforms to investigate capacitive charge based control of biofouling

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    Implantable devices, in particular biosensors, have clear utility within medicine, but face a hurdle to long-term function due to adsorption of biomolecules (biofouling) and subsequent immune re- sponse to implants, the foreign body response (FBR). Strategies to control this immune reaction have included material selection, drug release and, more recently, engineered surface properties. The increasing use of embedded electronics within many classes of implanted devices presents an opportunity to exploit electromagnetic phenomena at the device surface to mitigate biofouling and FBR. Such active biomaterials would allow dynamic modification of the apparent material properties of an implanted device. A hypothesis was developed that biological interaction with a biomaterial surface can be altered by capacitive charging. A platform was constructed to test this and related hypotheses around cell and protein surface interactions in vitro and adapted into a second platform for initial characterisa- tion work on an early in vivo model using chick eggs. These platforms were designed to be easy to fabricate and to provide multiple electrical connections into a substrate in contact with biological solutions or tissue. Electrodes were fabricated from fluoropolymer coated tantalum pentoxide, a high-Îș dielectric, and compared against adjacent, identically coated, silicon dioxide regions. Cells from the MDA- MB-231 cancer cell line were cultured on these regions under electrical stimulation. A voltage de- pendent reduction of cell attachment and spreading was detected on capacitively charged surfaces compared to uncharged controls. The tentative results, suggest capacitively charged surfaces hold promise as active biomaterials. A second cell type MCF-7 did not reproduce the effect, implying a more coherent understanding is required of the mechanisms behind cell surface interactions on these surfaces. Multiple independent bioelectrochemical cell-surface interactions were observed using the plat- form and several quantification techniques were successfully employed. It is therefore argued that the platform may have wide applicability as a future research tool

    The Palaces of Comfort, Consolation and Distraction - The Pie and Mash shop as a performative space of a contested London working class memory

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    This thesis seeks to interrogate and clarify the history and culture of London’s traditional but fading and largely forgotten eel, pie and mash shops. In doing so the work examines their cultural conduit, the adjacent and evolving identity of the cockney whose contested memoryscapes have, I suggest, great contemporary political and cultural relevance in an age of populism and Brexit. The work excavates a tracing around the shops’ absences in historical literature. It situates their establishment within the dying breath of an older, popular street culture and the birth of a new London working class, centred around unofficial street markets and in a synchronous dance with the ideological accession of the bourgeoisie. The thesis employs the biological notion of a taxon to illustrate the shops’ evolution largely defined by the class-demotion of their clientele that mirrored the changing cartography of the city. By the late nineteenth century, this work argues, the eel and pie shops had become a pillar of a respectable London working class culture whose hyper-local solidarities revolved around micro-class divisions of work and negotiated bourgeois codes of propriety as part of a ‘culture of consolation’ that has remained largely impenetrable to outsiders. The study explores this concomitant cockney identity which became, partly through bourgeois theatrical ventriloquising, a figure of imperial incorporation. This eventually came to represent a particular type of ‘ordinariness’, subsequently reconfigured around the gains of a Welfare State and a national economy that continues to be periodically valorised according its usefulness to capital at times of political stress. Utilising sensory ethnography and memory studies the work explores the landscape and territoriality of the contemporary eel, pie and mash shop. It interrogates the rituals and complex, often competing and polyphonic memory inscriptions which memorialise a largely post-colonial nostalgic melancholia around the loss of fantasy of a British omnipotence. The thesis argues that the shops and their simulacra-like reincarnations amongst the cockney diaspora in the Essex new towns offer an insight into the changing notions of taste and class within the convivialities of a unique but broadly closed heritage of proletarian culture as a zone of resistance in the neoliberal city

    Proceedings of FORM 2022. Construction The Formation of Living Environment

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    This study examines the integration of building information modelling (BIM) technologies in operation & maintenance stage in the system of managing real estate that helps to reduce transaction costs. The approach and method are based on Digital Twin technology and Model Based System Engineering (MBSE) approach. The results of the development of a service for digital facility management and digital expertise are presented. The connection between physical and digital objects is conceptualized

    Efficient instance and hypothesis space revision in Meta-Interpretive Learning

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    Inductive Logic Programming (ILP) is a form of Machine Learning. The goal of ILP is to induce hypotheses, as logic programs, that generalise training examples. ILP is characterised by a high expressivity, generalisation ability and interpretability. Meta-Interpretive Learning (MIL) is a state-of-the-art sub-field of ILP. However, current MIL approaches have limited efficiency: the sample and learning complexity respectively are polynomial and exponential in the number of clauses. My thesis is that improvements over the sample and learning complexity can be achieved in MIL through instance and hypothesis space revision. Specifically, we investigate 1) methods that revise the instance space, 2) methods that revise the hypothesis space and 3) methods that revise both the instance and the hypothesis spaces for achieving more efficient MIL. First, we introduce a method for building training sets with active learning in Bayesian MIL. Instances are selected maximising the entropy. We demonstrate this method can reduce the sample complexity and supports efficient learning of agent strategies. Second, we introduce a new method for revising the MIL hypothesis space with predicate invention. Our method generates predicates bottom-up from the background knowledge related to the training examples. We demonstrate this method is complete and can reduce the learning and sample complexity. Finally, we introduce a new MIL system called MIGO for learning optimal two-player game strategies. MIGO learns from playing: its training sets are built from the sequence of actions it chooses. Moreover, MIGO revises its hypothesis space with Dependent Learning: it first solves simpler tasks and can reuse any learned solution for solving more complex tasks. We demonstrate MIGO significantly outperforms both classical and deep reinforcement learning. The methods presented in this thesis open exciting perspectives for efficiently learning theories with MIL in a wide range of applications including robotics, modelling of agent strategies and game playing.Open Acces

    Mobile Forensics – The File Format Handbook

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    This open access book summarizes knowledge about several file systems and file formats commonly used in mobile devices. In addition to the fundamental description of the formats, there are hints about the forensic value of possible artefacts, along with an outline of tools that can decode the relevant data. The book is organized into two distinct parts: Part I describes several different file systems that are commonly used in mobile devices. · APFS is the file system that is used in all modern Apple devices including iPhones, iPads, and even Apple Computers, like the MacBook series. · Ext4 is very common in Android devices and is the successor of the Ext2 and Ext3 file systems that were commonly used on Linux-based computers. · The Flash-Friendly File System (F2FS) is a Linux system designed explicitly for NAND Flash memory, common in removable storage devices and mobile devices, which Samsung Electronics developed in 2012. · The QNX6 file system is present in Smartphones delivered by Blackberry (e.g. devices that are using Blackberry 10) and modern vehicle infotainment systems that use QNX as their operating system. Part II describes five different file formats that are commonly used on mobile devices. · SQLite is nearly omnipresent in mobile devices with an overwhelming majority of all mobile applications storing their data in such databases. · The second leading file format in the mobile world are Property Lists, which are predominantly found on Apple devices. · Java Serialization is a popular technique for storing object states in the Java programming language. Mobile application (app) developers very often resort to this technique to make their application state persistent. · The Realm database format has emerged over recent years as a possible successor to the now ageing SQLite format and has begun to appear as part of some modern applications on mobile devices. · Protocol Buffers provide a format for taking compiled data and serializing it by turning it into bytes represented in decimal values, which is a technique commonly used in mobile devices. The aim of this book is to act as a knowledge base and reference guide for digital forensic practitioners who need knowledge about a specific file system or file format. It is also hoped to provide useful insight and knowledge for students or other aspiring professionals who want to work within the field of digital forensics. The book is written with the assumption that the reader will have some existing knowledge and understanding about computers, mobile devices, file systems and file formats
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