A CMOS Tracking System Approach for Cell Motility Assays

This work proposes a method for studying and monitoring in real-time a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate Impedance Spectroscopy (ECIS) has been developed. From this cell model, obtained from experimental assays data, an algorithm based on analysis of the 8 nearest neighbors has been implemented, allowing the evaluation of the cell center of mass. The path followed by a cell, proposing a Brownian route, has been simulated with the proposed algorithm. The presented results show the success of the approach, with accuracy over 95% in the determination of any coordinate (x, y) from the expected center of mass.Ministerio de Economía y Competitividad TEC2013-46242-C3-1-

Cytotoxicity studies of lung cancer cells using impedance biosensor

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. To employ ECIS, the cells need to attach, spread and proliferate on the sensor in the presence of adhesion-promoting protein that mimics the extracellular matrix (ECM) of the cells. For cell attachment, collagen I, Bovine had been used as the coating substrate. In this study, four designs with varying electrode distances had been measured to detect the changes in impedance values of Lung Carcinoma cell lines (A549). The impedance change due to the cell growth and attachment was modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. The impedance measurements were measured every 8 hours for 120 hours at frequencies of 100Hz to 10MHz using Agilent Precision Impedance Analyzer 4294A. The experimental results have shown that the closest distance of the electrode gave the most optimum impedance value for A549 cancer cell’s measurement. The cancer cells were also treated with a chemotherapeutic drug, Taxol and its impedance response was monitored over 5 days. Experimental results show that there is significant reduction in impedance when the cancer cells were exposed to Taxol, indicating that the cells are no longer adherent to the sensor’s surface or are dead