This work proposes a method for studying and monitoring in real-time a single cell on a 2D electrode
matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS
system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate
Impedance Spectroscopy (ECIS) has been developed. From this cell model, obtained from experimental
assays data, an algorithm based on analysis of the 8 nearest neighbors has been implemented, allowing the
evaluation of the cell center of mass. The path followed by a cell, proposing a Brownian route, has been
simulated with the proposed algorithm. The presented results show the success of the approach, with
accuracy over 95% in the determination of any coordinate (x, y) from the expected center of mass.Ministerio de Economía y Competitividad TEC2013-46242-C3-1-
