2,585 research outputs found
The structures of secretory and dimeric immunoglobulin A
Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA that is bound by the polymeric Ig-receptor ectodomain, called secretory component (SC). Here, we report the cryo-electron microscopy structures of murine SIgA and dIgA. Structures reveal two IgAs conjoined through four heavy-chain tailpieces and the JC that together form a β-sandwich-like fold. The two IgAs are bent and tilted with respect to each other, forming distinct concave and convex surfaces. In SIgA, SC is bound to one face, asymmetrically contacting both IgAs and JC. The bent and tilted arrangement of complex components limits the possible positions of both sets of antigen-binding fragments (Fabs) and preserves steric accessibility to receptor-binding sites, likely influencing antigen binding and effector functions
Towards an Iterative Algorithm for the Optimal Boundary Coverage of a 3D Environment
This paper presents a new optimal algorithm for locating a set of sensors in 3D able to see the boundaries of a polyhedral environment. Our approach is iterative and is based on a lower bound on the sensors' number and on a restriction of the original problem requiring each face to be observed in its entirety by at least one sensor. The lower bound allows evaluating the quality of the solution obtained at each step, and halting the algorithm if the solution is satisfactory. The algorithm asymptotically converges to the optimal solution of the unrestricted problem if the faces are subdivided into smaller part
The structures of secretory and dimeric immunoglobulin A
Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA that is bound by the polymeric Ig-receptor ectodomain, called secretory component (SC). Here, we report the cryo-electron microscopy structures of murine SIgA and dIgA. Structures reveal two IgAs conjoined through four heavy-chain tailpieces and the JC that together form a β-sandwich-like fold. The two IgAs are bent and tilted with respect to each other, forming distinct concave and convex surfaces. In SIgA, SC is bound to one face, asymmetrically contacting both IgAs and JC. The bent and tilted arrangement of complex components limits the possible positions of both sets of antigen-binding fragments (Fabs) and preserves steric accessibility to receptor-binding sites, likely influencing antigen binding and effector functions
Recommended from our members
The MVP sensor planning system for robotic vision tasks
The MVP (machine vision planner) model-based sensor planning system for robotic vision is presented. MVP automatically synthesizes desirable camera views of a scene based on geometric models of the environment, optical models of the vision sensors, and models of the task to be achieved. The generic task of feature detectability has been chosen since it is applicable to many robot-controlled vision systems. For such a task, features of interest in the environment are required to simultaneously be visible, inside the field of view, in focus, and magnified as required. In this paper, we present a technique that poses the vision sensor planning problem in an optimization setting and determines viewpoints that satisfy all previous requirements simultaneously and with a margin. In addition, we present experimental results of this technique when applied to a robotic vision system that consists of a camera mounted on a robot manipulator in a hand-eye configuration
3-D Shape Matching for Face Analysis and Recognition
The aims of this paper are to introduce a 3-D shape matching scheme for automatic face recognition and to demonstrate its invariance to pose and facial expressions. The core of this scheme lies on the combination of non-rigid deformation registration and statistical shape modelling. While the former matches 3-D faces regardless of facial expression variations, the latter provides a low-dimensional feature vector that describes the deformation after the shape matching process, thereby enabling robust identification of 3-D faces. In order to assist establishment of accurate dense point correspondences, an isometric embedding shape representation is introduced, which is able to transform 3-D faces to a canonical form that retains the intrinsic geometric structure and achieve shape alignment of 3-D faces independent from individual’s facial expression. The feasibility and effectiveness of the proposed method was investigated using standard
publicly available Gavab and BU-3DFE databases, which contain faces expressions and pose variations. The performance of the system was compared with the existing benchmark approaches and it demonstrates that the proposed scheme provides a competitive solution for the face recognition task with real-world practicality
- …