Срок эксплуатации и продуктивность земляники садовой в условиях Белгородской области

В условиях Ботанического сада БелГУ изучена продуктивность 25 сортов земляники садовойyesБелгородский госуниверсите

Предпосылки получения безвирусной земляники в Белгородской области

Изучены технологии клонального микроразмножения и регенерации плодовых растений из различных органов и тканей для использования в системе производства оздоровленного посадочного материала и ускорения селекционного процесс

Identification of <i>Rca2</i> and <i>Rpf1</i> resistance alleles in strawberry breeding forms of intervarietal hybrid origin

Background. The creation of varieties with genetic resistance to fungal pathogens is one of the most important directions in strawberry breeding. A promising direction of accelerated selection of valuable specimens is molecular screening for the presence in their genotype of diagnostic DNA markers, linked to target genes of agrobiological traits. Objectives of the work were determination of the allelic state of the Rca2 (resistance to anthracnose) and Rpf1 (resistance to red stele root rot) genes in selected strawberry forms to identify genotypes resistant to Colletotrichum acutatum Simmonds and Phytophthora fragariae var. fragariae Hickman.Material and methods. The object of research was breeding forms of strawberries of intervarietal hybrid origin. As a result of 22 crossing combinations, 61 selection forms were obtained, in which the allelic composition of resistance genes was studied. The Rca2 anthracnose resistance allele was identified by means of the STS-Rca2_240 marker. The Rpf1 red stele root rot resistance allele was identified with the help of SCAR-R1A marker. Results. At least one of the two genes was identified in 20 selected strawberry forms (32.8%). The STS-Rca2_240 marker was identified in 15 strawberry seedlings (22.9%) from six hybrid combinations ‘Vicoda’ × ‘Roxana’, ‘Florence’ × ‘Faith’, ‘Asia’ × ‘Aprica’, ‘San Andreas’ × ‘Monterey’, ‘Alisa’ × ‘Quicky’, ‘Quicky’ × ‘Olympia’). The SCAR-R1A marker was identified in five strawberry seedlings (8.2%) from four hybrid combinations (‘Privlekatelnaya’ × ‘Bylinnaya’, ‘Feyerverk’ × ‘Bylinnaya’, ‘Bylinnaya’ × ‘Feyerverk’, ‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’). It has been established that all strawberry selected forms with identified marker fragments for resistance genes are characterized by a heterozygous combination of alleles.Conclusions. Promising sources of resistance alleles are strawberry selected forms: 2/1-24, 2/1-34 (‘Quicky’ × ‘Olympia’), 3/9-5 (‘Florence’ × ‘Faith’), 3/12-2, 3/12- 9 (‘Alisa’ × ‘Quicky’), 4/7-10, 4/7-19, 4/7-20 (‘Asia’ × ‘Aprica’), 5/2-26, 5/2-32 (‘San Andreas’ × ‘Monterey’), 9/ 1-11, 9/1-12, 9/1-13, 9/1-32, 9/1-37 ‘Vicoda’ × ‘Roxana’) in case of Rca2 gene; 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 69-29 (‘Feyerverk’ × ‘Bylinnaya’), 62-41 (‘Bylinnaya’ × ‘Feyerverk’), 61-15 (‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’) in case of Rpf1 gene

Analysis of the inheritance of the marker SCAR-R1A, linked to the <i>Rpf1</i> red stele root rot resistance gene, in strawberry hybrid progeny

Background. Resistance to pathogens is an important breeding trait of a cultivar. Red stele root rot (Phytophthora fragariae var. fragariae Hickman) is a dangerous root disease. Revealing the patterns of resistance inheritance and identifying promising genotypes is an important stage in the development of strawberry cultivars resistant to red stele root rot. The purpose of the study was to identify patterns of inheritance for the SCAR-R1A marker, linked to the Rpf1 red stele root rot resistance gene, in the strawberry hybrid combinations.Materials and methods. The target materials were the strawberry cultivars ‘Bylinnaya’, ‘Olimpiyskaya Nadezhda’, ‘Privlekatelnaya’ and ‘Feyyerverk’, and hybrid seedlings of the cross combinations Bylinnaya × Olimpiyskaya Nadezhda, Bylinnaya × Feyyerverk, Olimpiyskaya Nadezhda × Bylinnaya, Privlekatelnaya × Bylinnaya, and Feyyerverk × Bylinnaya. The Rpf1 gene was identified with the marker SCAR-R1A.Results and conclusion. For the hybrid combination Bylinnaya × Olimpiyskaya Nadezhda, the percentage of seedlings with an Rpf1 resistance allele was 33.3%. For the combination Bylinnaya × Feyyerverk, their percentage was 37.2%; for Olimpiyskaya Nadezhda × Bylinnaya, 39.4%; for Privlekatelnaya × Bylinnaya, 39.6%; and for Feyyerverk × Bylinnaya, 36.2%. The average percentage of seedlings with an Rpf1 allele for the studied combinations was 37.1%. Assessment of the compliance between the observed segregation and theoretical one according to the χ2  criterion confirmed the monogenic character of the studied trait and the Mendelian ratio of inheritance frequencies for the marker fragments of the Rpf1 gene as 1 : 1. Therefore, all identified seedlings with an Rpf1 allele are characterized by a heterozygous genotype. Strawberry hybrids promising for breeding were identified: 62-41 (Bylinnaya × Feyyerverk), 65-17, 65-24 (Olimpiyskaya Nadezhda × Bylinnaya), and 69-29 (Feyyerverk × Bylinnaya)

«Сие пишет человек, не видавший никогда фейерверк»: об одном анонимном стихотворении второй половины XVIII века

The article presents the text of a curious anonymous poem from a mid-18th-century manuscript, and analyses its genre and language features. Comparing the poem with the descriptions of fireworks published in the last years of Elizabeth Petrovna's reign allows us to make an assumption about the most likely date of writing and the circle of persons who could be associated with the anonymous author. The lexical features of the work, as it seems, indicate that the author was part of the circle of I.I. Shuvalov, P.I. Shuvalov and M.V. Lomonosov (which is confirmed by analysing the contents of the manuscript).The anonymous poem provides us with an understanding of how the reception of key components of court culture (fireworks, court holidays, allegorical figures, panegyric poems, etc.) looked for the noble society of the middle of the 18th century. DOI: 10.31168/2305-6754.2023.1.09В статье публикуется текст анонимного стихотворения из рукописного сборника середины XVIII в.; анализируются его жанровая природа и особенности языка. Сопоставление с публикациями описаний фейерверков последних лет правления Елизаветы Петровны позволяет сделать предположение о наиболее вероятной дате создания этого произведения и возможном круге лиц, с которыми был связан анонимный автор. Лексические особенности произведения позволяют предположить, что автор был связан с кругом И. И. Шувалова, П. И. Шувалова и М. В. Ломоносова (что подтверждается составом сборника, в котором сохранилось произведение). Это анонимное стихотворение — интересный пример осмысления различных поэтических и прозаических традиций, связанных с культурой фейерверка 2-й половины XVIII в.  DOI: 10.31168/2305-6754.2023.1.0

Особенности репрезентации явления «Успех» (на материале русcкоязычной газетной статьи)

Aleksandrova, T. L. Features of the representation of the phenomenon "Success" (based on the Russian-language newspaper article)ЯЗЫКОЗНАНИ

LIGHT IN THE WORKS OF GIACOMO BALLA IN THE PERSPECTIVE OF LIGHT ART GENESIS

The article is devoted tо the specific question in Russian art history – to the problem of light in the works of J. Balla from the perspective of Light art genesis. The insufficiently researched genesis of light art .in Modernism and Italian Futurism is followed by the incomplete understanding of phenomenon. This circumstance requires an appeal to the historical roots of Light art, particularly, to the period when artists started including artificial light into the practice of fine art and started using it as a medium. As a result of the research in the art of J. Balla the genesis of Light art has been revealed. The article examines the consistent change in the approach to light in the main futuristic manifestos and works of art. In Balla’s paintings of the 1910s, light was used as an image and element of art, while in plastic complexes and scenography of the theatrical production “Fireworks” it was used as a material (medium). The ballet “Fireworks” is proposed to be considered as prototypical work of light art. The results of the study can be used in the art historical research and cultural analysis of the history and modernity of light art. The research methodology is based on the art historical analysis of texts and works of art, as well as on the comparative method

Победа команды фармацевтического факультета ВГМУ в VI химико-олимпийских играх

МЕДИЦИНСКИЕ УЧЕБНЫЕ ЗАВЕДЕНИЯФАРМАЦЕВТИЧЕСКИЕ ФАКУЛЬТЕТЫХИМИКО-ОЛИМПИЙСКИЕ ИГРЫОБРАЗОВАТЕЛЬНЫЕ ИГРЫСТУДЕНТЫ МЕДИЦИНСКИХ УЧЕБНЫХ ЗАВЕДЕНИ

Genetic diversity in wild species and cultivars of strawberry for the FanAAMT gene controlling fruit flavor volatiles

Background. An important consumer trait of strawberry fruits is their aroma. Methyl anthranilate makes a considerable contribution to the fruit flavor. The methyl anthranilate content in strawberry fruits is controlled by the FanAAMT (аnthranilic acid methyltransferase) gene. Identification of genetic determinants for this trait facilitates targeted selection of promising forms based on diagnostic DNA markers. The purpose of this study was to study the allelic diversity of the FanAAMT fruit flavor gene in wild strawberry species and strawberry cultivars for identification of promising genotypes.Materials and methods. The objects of this study were wild species of Fragaria L. as well as F. × anaschata Kantor. and F. × ananassa Duch. cultivars of different ecogeographic origin. The FanAAMT gene was identified with the dominant marker FanAAMT.Results and conclusion. In the analyzed collection of strawberry genotypes, the FanAAMT gene was identified in 36.4% of the forms, including the wild species F. vesca L., F. moschata Duch. and F. virginiana subsp. platypetala (Rydb.) Staudt, and cv. ‘Kupchikha’ (F. × anaschata). Among the analyzed F. × ananassa cultivars, the FanAAMT gene was found in 31.6% of the tested forms, specifically in 27.3% of the 22 Russian cultivars, and 37.5% of the analyzed foreign ones. Promising sources of high methyl anthranilate content in fruits were identified: wild spp. F. moschata, F. vesca, and F. virginiana subsp. platypetala; cv. ‘Kupchikha’ (F. × anaschata); Russian garden strawberry cvs. ‘Bylinnaya’, ‘Zenit’, ‘Lastochka’, ‘Neznakomka’, ‘Feyerverk’ and ‘Tsarskoselskaya’; and foreign garden strawberry cvs. ‘Karmen’, ‘Ostara’, ‘Samson’, ‘Symphony’, ‘Troubadour’ and ‘Vima Tarda’, in which the functional allele of the FanAAMT gene was found. In the remaining studied strawberry genotypes the marker FanAAMT was not detected, which presumably indicates that the FanAAMT gene is absent. cells (R9.4.1)

Allelic diversity of the <i>FaOMT</i> gene (mesifurane biosynthesis) in promising strawberry cultivars and selected forms developed at the I.V. Michurin Federal Science Center

Background. Fruit aroma is an important consumer trait of strawberry varieties. Identification of genotypes carrying the genes of the aromatic complex in fruits is an important stage in the development of new cultivars with aromatic fruits. The purpose of this study was to analyze the allelic state of the FaOMT fruit aroma gene in strawberry cultivars and selected forms developed at the I.V. Michurin Federal Science Center to identify promising genotypes for use in breeding for fruit aroma.Materials and methods. The research materials were strawberry cultivars and promising selected forms developed at the I.V. Michurin FSC using the methods of introgressive and intervarietal hybridization. Allelic state of the FaOMT gene was identified with the codominant marker FaOMT-SI/NO.Results and conclusion. The FaOMT fruit aroma gene is represented in the studied strawberry varieties and forms by three combinations of alleles: the homozygous state of the functional allele, the heterozygous combination of the alleles, and the homozygous state of the nonfunctional allele. Cvs. ‘Rubinovy kaskad’, ‘Privlekatelnaya’ and ‘Feyerverk’ are characterized by a heterozygous combination of alleles of the FaOMT gene. Cvs. ‘Lastochka’, ‘Pamyati Zubova’, ‘Flora’, ‘Yarkaya’ and ‘Urozhaynaya CGL’ are characterized by the homozygous state of the FaOMT+ allele. Selected strawberry forms 928-12, 26-5, 72-71 and 62-41 have the heterozygous genotype; hybrids 298-19-9-43 and 932-29 are characterized by the homozygous state of the FaOMT+ allele; selected forms 61-12 and 65-26 are characterized by the homozygous state of the nonfunctional FaOMT– allele. Strawberry forms promising for breeding, with the homozygous state of the functional FaOMT+ allele, were identified: cvs. ‘Lastochka’, ‘Pamyati Zubova’, ‘Flora’, ‘Yarkaya’ and ‘Urozhaynaya CGL’, and selected forms 298-19-9-43 and 932-29