7 research outputs found

    Western blotting analysis of <i>L</i>. <i>lactis</i> NZ9000 expressing different <i>spaA-srtC</i>1 gene variants, where the GYPSY motif has been altered by AA substitution.

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    <p><b>SpaA pilin proteins were detected using anti-SpaA polyclonal antibodies.</b> Legend: #, protein weight marker; HMWL, high molecular weight ladder; *, band corresponding to elongated pilus structures.</p

    Electron microscopy observations of <i>Lb</i>. <i>rhamnosus</i> PB12 and its SrtC1-complemented derivatives.

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    <p>Bacterial cells were immunogold-labeled with anti-SpaA serum and gold particles (10 nm). Legend: A, <i>Lb</i>. <i>rhamnosus</i> GG; B, <i>Lb</i>. <i>rhamnosus</i> PB12; C, <i>Lb</i>. <i>rhamnosus</i> PB12 + SrtC1.</p

    Western blotting analysis of <i>L</i>. <i>lactis</i> MG1363 PPiA KO mutant and its complemented mutant, where the SpaA-SrtC1 cassette was introduced.

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    <p>SpaA pilin proteins were detected using anti-SpaA polyclonal antibodies. Legend: #, protein weight marker; HMWL, high molecular weight ladder; *, band corresponding to elongated pilus structures.</p

    Table_1_Facilitators and barriers of preventive behaviors against COVID-19 during Ramadan: A phenomenology of Indonesian adults.DOCX

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    IntroductionIntercity mobility restriction, physical distancing, and mask-wearing are preventive behaviors to reduce the transmission of COVID-19. However, strong cultural and religious traditions become particular challenges in Indonesia. This study uses the Behavior Change Wheel to explore barriers and facilitators for intercity mobility restriction, physical distancing, and mask-wearing during Ramadan.MethodsSemi-structured in-depth interviews with 50 Indonesian adults were conducted between 10 April and 4 June 2020. Having mapped codes into the Capacity, Opportunity, Motivation ÔÇô Behavior (COM-B), and Theoretical Domain Framework (TDF) model, we conducted summative content analysis to analyze the most identified factors to preventive behaviors and proposed interventions to address those factors.ResultsBelief about the consequence of preventive behaviors was the most mentioned facilitator to all preventive behaviors among compliers. However, optimism as a TDF factor was commonly mentioned as a barrier to preventive behaviors among non-compliers, while environmental context and resources were the most commonly mentioned factors for intercity mobility restriction.ConclusionsPublic health intervention should be implemented considering the persuasion and involvement of religious and local leaders. Concerning job and economic context, policy related to the intercity mobility restriction should be reconsidered to prevent a counterproductive effect.</p
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