The one-dimensional (1D) ddPCR results from Bio-Rad QX100TM Droplet Reader of <i>18S rRNA Plasmodium</i> genus detection and quantification assay.

<p>The different amplitudes of positive droplets were observed when different PCR annealing temperatures were applied (A10: 65°C, D10: 62°C, E10: 60°C, F10: 59°C, H10: 57°C, F09: 60°C for negative control). Threshold for positive detection is 2500.</p

Probit analysis.

<p>The 95% probability of detecting parasitaemia as low as 10.674 parasites/mL when high volume of blood samples was applied with <i>Plasmodium</i> genus ddPCR assay.</p

Workflow of the study.

<p>Workflow of the study.</p

Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).

<p>Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).</p

The column bar graphs show <i>18S rRNA</i> copies/mL of blood obtained from single reaction ddPCR and duplex ddPCR assays.

<p>Mean 95% CI of <i>18S rRNA</i> of <i>P</i>. <i>falciparum</i> (5a), <i>P</i>. <i>vivax</i> (5b), <i>P</i>. <i>malariae</i> (5c) and <i>P</i>. <i>ovale</i> (5d).</p

Summary of perceived delays affecting the decision to attend referral hospital, and delays to transport after this decision.

<p>Summary of perceived delays affecting the decision to attend referral hospital, and delays to transport after this decision.</p

Multiple linear regression analyses of pre-hospital illness timespan (in hours) for adults, children, and all participants.

<p>Multiple linear regression analyses of pre-hospital illness timespan (in hours) for adults, children, and all participants.</p

MPI score <i>vs</i>. average household income per adult equivalent per month.

<p>The correlation is significant with a P-value of <0.001 and a Spearman’s rho of -0.37. Thresholds for MPI and US$2.00 daily income per adult equivalent (adjusted to the World Bank’s private individual purchasing power parity estimate) are shown for reference. 56$2.00/adult/day, and 51% fall below the MPI threshold for classification as multidimensionally poor.</p

Sources of payment for expenses arising from illness before arrival at the referral hospital.

<p>Sources of payment for expenses arising from illness before arrival at the referral hospital.</p

Poverty and AFI Dataset

Data underlying the publication titled, "The relationship between poverty and healthcare seeking among patients hospitalized with acute febrile illnesses in Chittagong, Bangladesh.