Numerical simulations of non-fluorescent states of carboxyfluoresceins in presence of heavy iodine ions

Abstract

In the present research, the effects of heavy iodine ions on dark transient state populations of carboxyfluorescein derivatives, each with a different number of bromine atoms attached, were computationally examined via numerical simula tions adapted to a widefield fluorescence microscopy integrated with a microfluidics platform. Numerical simulations were car ried out by considering geometrical profile of excitation laser beam, microscopy and microfluidics parameters of a proposed experimental design as well as electronic transition rates of fluorescent molecules. Electronic state model of studied dyes was treated as a system of first order ordinary differential equa tions and time-dependent solutions of long-lived, non-fluorescent dark state populations were computed for each dye at varying potassium-iodine [KI] concentrations. Analytical solutions of state populations were then adapted to a proposed experimental setup to systematically analyze how dark state populations evolve when carboxyfluorescein derivatives pass through exci tation beam field in a microfluidics chip under different flow speeds. Computational experiments have successfully uncovered systematic changes in dark triplet and photo-oxidized state populations upon the addition of iodine ions into fluorophore solutions.TÜBİTAKPost prin