Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang
Doi
Abstract
The aim of this research is to isolate and characterize lactic acid bacteria from mare's milk which have the potential to act as probiotics. Bacterial identification includes gram staining, antimicrobial activity test, hemolytic activity test, pH and bile salt tolerance test. The research results showed that C5 isolates isolated from horse milk samples had potential as probiotics. Characterization includes the form of bacillary bacteria, antimicrobial activity against the pathogenic bacteria Escherichia coli ATCC of 0.40 mm, gamma hemolysis, tolerance to pH 2, 3, 5 and tolerance to bile salts of 0.4-1%.Tujuan dari penelitian ini adalah mengisolasi dan karakterisasi bakteri asam laktat dari susu kuda yang berpotensi sebagai probiotik. Identifikasi bakteri meliputi pewarnaan gram, uji aktivitas antimikroba, uji aktivitas hemolitik, uji toleransi terhadap pH dan garam empedu. Hasil penelitian menunjukkan isolat C5 yang diisolasi dari sampel susu kuda memiliki potensi sebagai probiotik. Karakterisasi meliputi bentuk bakteri basil, memiliki aktivitas antimikroba terhadap bakteri patogen Escherichia coli ATCC sebesar 0,40 mm, bersifat gamma hemolisis, toleransi terhadap pH 2, 3, 5 dan toleransi terhadap garam empedu 0,4-1%
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