GENOTYPIC DIFFERENCES IN THE PHYSIOLOGICAL RESPONSE OF MAIZE SEEDLINGS TO WATER WITHHOLDING

Abstract

Ograničena dostupnost vode jedan je od glavnih čimbenika oksidativnog stresa u biljkama, a u takvim uvjetima nastaju reaktivne kisikove jedinke (ROS) koje oštećuju biološke membrane i stanične strukture. Cilj ovog istraživanja bio je odrediti kako stres uvjetovan zasušivanjem utječe na fiziološki odgovor različitih genotipova klijanaca kukuruza (Zea mays L.). Zasušivanje je u većini genotipova izazvalo povećanje koncentracije lipidne peroksidacije, vodikovog peroksida i sadržaja prolina. Rezultati ovog istraživanja ukazuju da je odgovor na stres zasušivanjem ovisan o genotipu kukuruza te da možemo razlikovati genotipove kukuruza s većom tolerancijom na zasušivanje i one osjetljivije. Potrebna su daljnja istraživanja antioksidacijskog sustava i mjerenja drugih komponenti oksidacijskog stresa kako bi se utvrdila povezanost između količine ROS-a i antioksidacijskog odgovora.Limited water availability is one of the main factors of oxidative stress in plants. In such conditions formation of reactive oxygen species (ROS) can damage biological membranes and cell structures. The aim of this study was to determine how water withholding stress affected the physiological response of different maize seedlings (Zea mays L.) genotypes. Water withholding caused an increase in the concentration of lipid peroxidation, hydrogen peroxide and proline content in most genotypes. The results of this study indicate that the response to water withholding stress in maize is genotype-dependent and that we can distinguish maize genotypes with higher tolerance and those more sensitive to stress. Further studies of the antioxidant system and measurements of other components of oxidative stress are needed in order to determine the relationship between the amount of ROS and the antioxidant response

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