In previous works, our research group has successfully proved the use of subcellular vaccines
based on poly(ε-caprolactone) (PEC) microparticles containing an antigenic extract of Brucella ovis (HS)
against experimental brucellosis in both mice and rams. However, the successful exploitation of
pharmaceutical products, and therefore of this product as veterinary vaccine, requires preservation of
both biological activity and native structure in all steps of development from purification to storage. In
this context, we have carried out an accelerated stability study to evaluate the relative stability of HS
when loading in PEC microparticles. For this purpose, freeze-dried microparticles were stored at 40±1°C
and 75% RH as a preliminary analysis of a stability testing. The results showed that both physicochemical
(size, morphology, antigen content, release profile) and biological (integrity and antigenicity of
the HS) properties were preserved after 6 months of storage. On the contrary, after 1 year of storage, the
HS release profile was dramatically affected probably due to a progressive loss of the polymer
microstructure. In addition, the degradation and loss of the antigenicity of the HS components was also
evident by SDS-PAGE and immunoblotting analysis. In fact, after 12 months of storage, only the
integrity and antigenicity of two of the major protective proteins of the HS antigenic complex were
preserved
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