The
detection and quantification of short nucleic acid sequences has many
potential applications in studying biological processes, monitoring
disease initiation and progression, and evaluating environmental systems,
but is challenging by nature. We present here an assay based on the
solid-state nanopore platform for the identification of specific sequences
in solution. We demonstrate that hybridization of a target nucleic
acid with a synthetic probe molecule enables discrimination between
duplex and single-stranded molecules with high efficacy. Our approach
requires limited preparation of samples and yields an unambiguous
translocation event rate enhancement that can be used to determine
the presence and abundance of a single sequence within a background
of nontarget oligonucleotides
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