<p>Although the human ULK complex mediates phagophore initiation similar to the budding yeast <i>Saccharomyces cerevisiae</i> Atg1 complex, this complex contains ATG101 but not Atg29 and Atg31. Here, we analyzed the fission yeast <i>Schizosaccharomyces pombe</i> Atg1 complex, which has a subunit composition that resembles the human ULK complex. Our pairwise coprecipitation experiments showed that while the interactions between Atg1, Atg13, and Atg17 are conserved, Atg101 does not bind Atg17. Instead, Atg101 interacts with the HORMA domain of Atg13 and this enhances the stability of both proteins. We also found that <i>S. pombe</i> Atg17, the putative scaffold subunit, adopts a rod-shaped structure with no discernible curvature. Interestingly, <i>S. pombe</i> Atg17 binds <i>S. cerevisiae</i> Atg13, Atg29, and Atg31 in vitro, but it cannot complement the function of <i>S. cerevisiae</i> Atg17 in vivo. Furthermore, <i>S. pombe</i> Atg101 cannot substitute for the function of <i>S. cerevisiae</i> Atg29 and Atg31 in vivo. Collectively, our work generates new insights into the subunit organization and structural properties of an Atg101-containing Atg1/ULK complex.</p
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