Sind i-Motiv-Strukturen in DNA kompatibel mit Cytosin–Ag(I)–Cytosin-Basenpaaren?

To evaluate whether the protons of hemi-protonated C:CH+ base pairs in DNA i-motif structures can be replaced by silver ions, an i-motif/duplex junction (IDJ) known to be stable at pH 7 was investigated. Interestingly, only the most accessible out of five C:CH+ pairs in the structure can be converted into a C–AgI–C base pair, as was derived by 1H NMR spectroscopy, luminescence spectroscopy, temperature-dependent UV spectroscopy, and isothermal titration calorimetry (ITC). When more than one silver ion is present per IDJ, dynamic light scattering (DLS) and CD spectroscopy indicate that the IDJ rearranges into a larger aggregated structure, likely to contain non-canonical silver-mediated base pairs. The study suggests that—in contrast to earlier reports—the DNA i-motif in general is not compatible with the formation of contiguous C–AgI–C pairs.Zur Untersuchung, ob die Protonen aus hemiprotonierten C:CH+-Basenpaaren in i-MotivStrukturen durch Silberionen ersetzt werden können, wurde ein i-Motiv/DNA-Konjugat (IDJ, engl. i-motif/duplex junction) untersucht, das auch bei pH 7 stabil ist. Interessanterweise kann nur das am besten zugängliche der fünf C:CH+- Basenpaare in der i-Motiv-Struktur in ein C–AgI –C-Basenpaar überführt werden, wie durch 1H-NMR-Spektroskopie, Lumineszenzspektroskopie, temperaturabhängige UV-Spektroskopie und isotherme Titrationskalorimetrie (ITC) belegt wurde. Liegt mehr als ein Silberion pro IDJ vor, zeigen Untersuchungen mittels dynamischer Lichtstreuung (DLS) und CD-Spektroskopie, dass sich das IDJ in eine größere aggregierte Struktur umwandelt, die vermutlich nicht-kanonische silbervermittelte Basenpaare enthält. Die Studie deutet darauf hin, dass – entgegen früherer Berichte – das i-Motiv in DNA im Allgemeinen nicht kompatibel mit der Ausbildung benachbarter C–AgI –C-Basenpaare ist.Deutsche Forschungsgemeinschaft (GRK 2027)NSERC (RGPIN-2022-03129)MCIN/AEI/10.13039/501100011033 (PID2020-120186RB-I00)Spanish Ministry of Education (FPU23/02032

INSANE_01_DMP_V1.0_WP5.pdf

DATA MANAGEMENT PLAN PROJECT INSANEEuropean Commission - MSCA-IF-EF-CAR (2020

Invitado en el programa Sentir Cofrade, 25 de marzo de 2025, presentado por Alejandro Morante

https://www.youtube.com/watch?v=HCeFZca8QTcExposición Eugenio Chicano, Rostros de la Pasión, en la Agrupación de Cofradías

Superset versus traditional resistance training prescriptions: a systematic review and meta‑analysis exploring acute and chronic effects on mechanical, metabolic, and perceptual variables

Background Supersets are a time-efficient resistance training (RT) method that involve the sequencing of two exercises with little or no rest between them. However, despite their common implementation during RT, a comprehensive and quantitative review is still lacking. Objectives The primary aim of this systematic review and meta-analysis was to compare the acute and chronic effects of superset and traditional set prescriptions on mechanical, metabolic, and perceptual variables. We also aimed to conduct subgroup analyses to determine the effect of different types of supersets (agonist–antagonist, similar biomechanical, and alternate peripheral supersets). Methods A systematic literature search was conducted in PubMed, Web of Science, Embase, and EBSCO databases from inception to 10 February 2024. Studies written in English and meeting our inclusion criteria were included. Pooled metaanalysis and subgroup meta-analysis were performed using a random-effects model. Results Nineteen studies involving 313 participants were included. Although there was considerable variance in certain outcomes, our estimated effects suggested that, compared with traditional set prescription, supersets allow for (1) a similar total number of repetitions [standardized mean differences (SMD) = − 0.03; p = 0.92] and volume load (SMD = 0.05; p = 0.86) with a shorter session duration and increased training efficiency (SMD = 1.74; p = 0.01); (2) higher blood lactate concentration during (SMD = 0.94; p = 0.03) and after (SMD = 1.13; p < 0.01) RT; (3) higher energy cost during RT (SMD = 1.93; p = 0.04); (4) similar creatine kinase concentration after RT (SMD = 0.22; p = 0.36), surface electromyography (SMD = 0.01; p = 0.98), acute muscle swelling (SMD = − 0.28; p = 0.36) and blood pressure (systolic blood pressure [SMD = 0.08; p = 0.71], diastolic blood pressure [SMD = − 0.05; p = 0.85], and mean arterial pressure [SMD = − 0.03; p = 0.88]); (5) higher rating of perceived exertion (SMD = 0.77; p = 0.02) and similar perceived recovery (SMD = 0.32; p = 0.33); and (6) similar chronic adaptations in maximal strength (SMD = 0.10; p = 0.36), strength endurance (SMD = 0.07; p = 0.81), and muscle hypertrophy (SMD = − 0.05; p = 0.87). The subgroup analysis revealed that utilizing agonist–antagonist supersets leads to a significant increase in the number of repetitions that are able to be completed compared with traditional sets (SMD = 0.68; p = 0.01). Similar biomechanical supersets led to less volume load (SMD = − 1.08; p < 0.01) compared with traditional sets. Conclusions Supersets provide a time-efficient alternative to traditional RT, reducing session duration without compromising training volume, muscle activation, perceived recovery, or chronic adaptations in maximal strength, strength endurance, and muscle hypertrophy. Thus, supersets can be effectively implemented by athletes with busy schedules and RT enthusiasts whose main barrier to exercise is time. However, it should be noted that supersets generally induce higher internal loads, more severe muscle damage, and increased perceived exertion, potentially necessitating extended recovery times between sessions. Additionally, superset RT may have a similar potential to traditional RT in eliciting post-exercise hypotension. Regarding different types of supersets, agonist–antagonist supersets are more suitable for maintaining training volume, while similar biomechanical supersets concentrate stimulation on the same muscle group, compromising volume load.Universidad de Granada/ CBU

Una investigación cualitativa sobre las plataformas digitales de aprendizaje adaptativo para (re)configurar las prácticas pedagógicas de los profesores de inglés como lengua extranjera (EFL) en China

Henan Province higher education teaching reform research and practice project “Student-centered Innovation and Entrepreneurship Course Model Exploration and Practice” (No.: 2021SJGL1025).Educational technologies have long been approved to influence several aspects of second/foreign language (L2) education. However, research on the impact of digital adaptive learning platforms/systems on L2 teaching is insufficient in the context of English as a foreign language (EFL). To address the gaps, this qualitative study intended to examine Chinese EFL teachers’ perceptions about the influence of such technologies on their pedagogical practices. A semi-structured interview was used to gather the data from 34 teachers. The results of thematic analysis indicated that digital adaptive learning platforms/systems had affected Chinese EFL teachers’ pedagogy in six venues of ‘teaching content’, ‘teaching methods’, ‘instructional resources’, ‘testing’, ‘classroom interactions’, and ‘classroom management’, which were found to be changing and enriching in light of such technologies. A detailed discussion of each finding is separately provided and implications for L2 teaching are enumerated to promote EFL teachers’ technological literacy, awareness, and practice.Durante mucho tiempo se ha reconocido que las tecnologías educativas influyen en varios aspectos de la enseñanza de lenguas segundas o extranjeras (L2). Sin embargo, en el contexto del inglés como lengua extranjera (EFL), la investigación sobre el impacto de las plataformas o sistemas de aprendizaje digital adaptativo en la enseñanza de L2 es insuficiente. Para cubrir estas lagunas, este estudio cualitativo tuvo como objetivo examinar las percepciones de los profesores de inglés como lengua extranjera (EFL) chinos sobre la influencia de tales tecnologías en sus prácticas pedagógicas. Se utilizó una entrevista semiestructurada para recopilar datos de 34 profesores. Los resultados del análisis temático indicaron que las plataformas o sistemas de aprendizaje digital adaptativo habían afectado la pedagogía de los profesores de inglés como lengua extranjera (EFL) chinos en seis ámbitos: 'contenido docente', 'métodos de enseñanza', 'recursos educativos', 'evaluación', 'interacciones en el aula' y 'gestión del aula', que se encontraron en proceso de cambio y enriquecimiento gracias a tales tecnologías. Se ofrece una discusión detallada de cada hallazgo por separado, y se enumeran las implicaciones para la enseñanza de L2 con el fin de promover la alfabetización, la conciencia y la práctica tecnológica de los profesores de inglés como lengua extranjera (EFL).Henan Province 2021SJGL102

Altérité et démesure : l’étrangère et la folle chez Adélaïde Blasquez

Dans cette étude, nous analysons les représentations de l’excès et de la démesure dans la narration de l’écrivaine française d’origine espagnole Adélaïde Blasquez, à travers trois figures récurrentes qui fonctionnent comme des alter egos de l’auteure : l’étrangère, l’écrivaine et la folle. Une attention particulière est portée à la représentation du corps comme lieu d’incarnation des violences subies par les femmes, mais aussi des formes de résistance qu’elles élaborent pour y faire face. Notre cadre théorique mobilise la notion d’abjection proposée par Julia Kristeva (1980), ainsi que la dichotomie du pur et de l’impur analysée par Mary Douglas (1973).En este estudio analizamos las representaciones del exceso y la desmesura en la narrativa de la escritora francesa de origen español Adélaïde Blasquez, a través de tres figuras recurrentes que funcionan como alter egos de la autora: la extranjera, la escritora y la loca. Se presta especial atención a la representación del cuerpo como lugar de encarnación de las violencias sufridas por las mujeres, así como de las formas de resistencia que ellas desarrollan para afrontarlas. Nuestro marco teórico se apoya en la noción de abyección propuesta por Julia Kristeva (1980) y en la dicotomía entre lo puro y lo impuro analizada por Mary Douglas (1973).This study examines the representations of excess and excessiveness in the narrative of the French writer of Spanish origin, Adélaïde Blasquez. Our analysis focuses on three recurrent figures that operate as the author’s alter egos: the foreign woman, the writer, and the madwoman. Particular attention is given to the body, conceived as a site where both the violences inflicted upon women and the strategies of resistance they devise are inscribed. The theoretical framework draws on Julia Kristeva’s (1980) notion of abjection, as well as on Mary Douglas’s (1973) analysis of the dichotomy between purity and impurity

Madres y niños sin pan. El hambre en las Cocinas de Hermandad y los Comedores Infantiles de Auxilio Social durante la posguerra franquista (1939-1940)

This research has been possible thanks to a grant IJC2020-046071-I funded by MCIN/AEI /10.13039/501100011033 and by the European Union NextGenerationEU/PRTR. Work produced with the support of a 2023 Leonardo Grant for Scientific Research and Cultural Creation, BBVA Foundation. The author is part of the following research projects: Heritages of Hunger: Societal Reflections on Past European Famines in Education, Commemoration and Musealisation (NWA. 1160.18.197; NWO; Radboud University/NIOD Institute) and La hambruna española: causas, desarrollo, consecuencias y memoria (1939-1952) (PID2019-109470GB-I00; Ministerio de Economía; Universidad de Granada), that have contributed to funding the research that supports this article.This article analyses one of the main social policies in post-war Spain: the welfare policies of Auxilio Social. In particular, it explores the conditions and daily operation of the Comedores Infantiles and Cocinas de Hermandad run by that Falangist institution in Madrid in the immediate postwar period (1939 and 1940). This work sustaines that the rations served in these charitable-assistance institutions were fewer, smaller, and of worse quality than officially recognised. Therefore, the food given to the needy mothers and children at these Auxilio Social premises was insufficient to quell their hunger. Moreover, since the facilities did not meet the minimum conditions of size, cleanliness, ventilation, and kitchen utensils, the attendees were exposed to infectious diseases. It is also argued that, beyond the food supply problems of those famine years, the ineffectiveness of the Comedores and Cocinas was often due to the staff who worked in them, as they often used to steal food for their own consumption or to sell on the black market. Finally, it is argued that, given its failure to feed the needy, Auxilio Social’s aim of extending support to the dictatorship through the guise of charity was tarnished.Este artículo analiza una de las principales políticas sociales de posguerra: el asistencialismo de Auxilio Social. En particular, explora las condiciones y el funcionamiento cotidiano de los Comedores Infantiles y las Cocinas de Hermandad de esta institución falangista en el Madrid de la inmediata posguerra (1939 y 1940). Se sostiene la tesis de que las raciones servidas en estas instituciones benéfico-asistenciales eran menos, más pequeñas y de peor calidad que las reconocidas oficialmente. Y que, por tanto, las madres y los niños asistidos no lograban apagar su hambre tras su paso por estos locales de Auxilio Social. Al contrario, al no reunir las instalaciones unas condiciones mínimas de tamaño, limpieza, ventilación o menaje los asistidos se exponían a contagios infecciosos. Asimismo, se defiende que, más allá de los problemas de suministros en aquellos años de hambre, la responsa- bilidad de la inoperancia de Comedores y Cocinas recayó a menudo en el personal que trabajaba en ellos, que acostumbraba a sustraer alimentos para su consumo particular o para su desvío al mercado negro. Por último, se sostiene que, dado su fracaso a la hora de dar de comer a los necesitados, el objetivo de Auxilio Social de extender el consentimiento hacia la dictadura mediante el reclamo de la caridad se vio empañado.MCIN/AEI/10.13039/501100011033 IJC2020-046071-IEuropean Union NextGenerationEU/PRTRBBVA FoundationPID2019-109470GB-I00Ministerio de EconomíaUniversidad de Granad

Understanding the application of digital technologies in aquaculture supply chains through a systematic literature review

The study conducts a systematic literature review of the application of digital technologies in aquaculture supply chains (ASC) to identify key research clusters, examine collaborative efforts in the field, and highlight emerging knowledge themes. The methodology comprises a database search in Scopus and Web of Science over a 5-year period (2019–2023) following the PRISMA framework. Bibliometric analysis using Biblioshiny reveals that “Climate Change,” “Aquaculture”, “Sustainability”, and “Food Security” were dominant keywords in this field. Notably, the Sustainability cluster exhibits the highest Callon Centrality (2.819) and Callon Density (63.378), underscoring significant research focus on integrating digital technologies to enhance sustainability in ASC. Hungary emerges as the country with the strongest international research collaboration. However, we identified weak collaboration between African nations and the global research community. Five primary research themes were identified; these include the role of digital technologies in ASC optimization, the disruption of fisheries supply chains due to the COVID-19 pandemic and the mitigating role of digital innovations, the contribution of digital technologies to reducing food waste and advancing the circular economy, the impact of climate change on fishes, and the challenges and opportunities in applying digital solutions within ASC. Despite persistent challenges— such as limited transmission bandwidth, network delays, and issues with low-power, longrange communication—significant opportunities exist to overcome these barriers through technological advances and stronger global research collaboration. Such progress is vital to transforming ASC into a more sustainable and competitive system. The study provides actionable insights for stakeholders while laying a foundation for future research and governance in this evolving field.European Union’s Horizon 2020- Marie Sklodowska-Curie-RISE (Project SUSTAINABLE, grant number 101007702

Treatment-resistant depression and intranasal esketamine: Spanish consensus on theoretical aspects

Introduction: Depression is a highly prevalent disease that severely impacts the life of patients. Inadequate response to at least two antidepressants despite adequacy of treatment and adherence is known as treatment-resistant depression (TRD), which entails a higher social and economic burden than non-resistant major depression. The lack of consensus on the definition of TRD and other aspects complicates management of the disease. Intranasal esketamine has a novel mechanism of action that differs from that of traditional antidepressants by improving neuroplasticity and synaptogenesis. Material and methods: A scientific committee comprising ten psychiatrists, experts in TRD in Spain, reviewed the literature (grey literature and articles or scientific communications published between January 2014 and January 2024 in PubMed) and developed statements on theoretical and conceptual aspects of TRD. Statements were developed in a first meeting following a discussion group approach, refined in a second meeting following a nominal group technique, and consensus was finally drafted in a third meeting. Results: A series of statements and recommendations were developed. Definitions for TRD and clinical response were proposed. The impact of therapeutic inertia was highlighted, identifying its causes and consequences. The role of intranasal esketamine in the TRD therapeutic treatment landscape was reviewed, and a treatment algorithm was developed, including specifics on evaluation of response to avoid therapeutic inertia and ensure an adequate treatment. Conclusions: This is the first consensus developed in Spain regarding theoretical aspects of TRD and the role of intranasal esketamine in TRD therapeutic approach. A definition of TRD was proposed, together with a treatment algorithm.Johnson & Johnso