An Efficient, Organic Solvent-Free Method for Extraction and Concentration of Hepatitis E Virus from Pig Liver

Author Contribution: Conceptualization, JSP and DRL; methodology, JSp, NG, DRL; validation, JSP; formal analysis,JSP, NG, DRL; investigation, JSP; writing—original draft preparation,JSP; writing—review and editing, DRL; visualization, JSP, DRL; supervision, DRL; project administration, DRL, funding acquisition, DRL. All authors have read and agreed the manuscript.The presence of the hepatitis E virus (HEV) in pork products, particularly in pig liver has been frequently described. However, a standardized method is not still available for the detection of HEV in foods, particularly in those difficult food matrixes such as pig meat and pork products. The aim of this study was to design, optimize and evaluate a new method of food-separation and virus concentration for HEV in pig liver samples. This method is based on organic flocculation and avoids the use of organic solvents. The virus recovery rates and analytical sensitivity of the method using murine norovirus MNV-1 as a surrogate were 73.6–82.2% and at least 1 × 103 TCID50 per g of liver in 100% of the replicates, respectively. Furthermore, this new methodology was validated by testing the presence of HEV RNA in naturally infected pig liver samples, comparing it with two other commonly used concentration methods. The new extraction method run satisfactory in comparison with the two reference methods; statistically equivalent (p < 0.05) to one of the methods used while presented statistically significant better results (p < 0.05) compared to the second method. Consequently, our results indicate that the new extraction method can be an adequate cost-effective and ecologically friendly alternative for the food separation and concentration of HEV RNA in pig liver samples.Ministerio de Ciencia, Innovación y Universidades (España)Junta de Castilla y LeónDepto. de Sanidad AnimalCentro de Vigilancia Sanitaria Veterinaria (VISAVET)TRUEpu

Using ensembles of dielectric nanoparticles in the FROG technique

CT63/19-CT64/19We have implemented the FROG (frequency-resolved optical gating) technique for the characterization of ultrashort laser pulses in the range of tens of femtoseconds using ensembles of dielectric nanoparticles as the non-linear material for SHG (second-harmonic generation). In order to measure traces that could be retrieved with standard retrieval algorithms, a collinear configuration was needed to avoid undesirable effects from scattered light. The amplitude and phase of the retrieved pulses are in agreement with those obtained using BBO as nonlinear material. The large non-linear signals emitted by ensembles of nanoparticles and their low cost set them as an interesting alternative to autocorrelation to characterize ultrashort laser pulses in different spectral ranges. Since optical fiber lasers are now providing short pulses around tens of femtoseconds in many different spectral ranges, this field could greatly benefit from our findings.Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Universidad Complutense de MadridDepto. de ÓpticaFac. de Ciencias FísicasTRUEpu

The old central igneous complexes of Sal, Boa Vista and Maio islands: Implications for 17 Ma of isotopic evolution of the Cape Verde archipelago

The central igneous complexes of the easternmost islands (Sal, Boa Vista and Maio) preserve some of the most ancient outcropping rocks of the Cape Verde (CV) archipelago. These Early to Middle Miocene (about 25 to 12 Ma) complexes show marked isotopic differences between mafic rocks from the northern (Sal and Boa Vista) and the southern Maio Island, the latter showing lower 143Nd/144Nd, 206Pb/204Pb, 207Pb/204Pb, and higher 87Sr/86Sr ratios. The main mantle plume composition beneath Cape Verde is here considered to be dominated by the FOZO component. Our data supports previous models suggesting the incorporation to plume components of minor DMM mantle sources in the northern CV island alignment, whereas mixing with the EM1 pole is prevalent on the southern CV islands. These isotopic differences are evident since the early stages of Cape Verde magmatism. The Late Miocene to Holocene time period (7 to 1.7 Ma) is characterized by the irruption of silica-undersaturated and carbonatite magmas with a relatively homogeneous isotopic composition and highly radiogenic Pb signatures (206Pb/204Pb up to 20.6 in carbonatitic rocks and 20.2 in silicate rocks) throughout most of the Cape Verde archipelago. During this transitional stage, the input of this new HIMU mantle component overprinted the previous mixing of the main FOZO plume component with shallow mantle members (DMM, EM1) of minor contribution.Ministerio de Ciencia e InnovaciónFundação para a Ciência e Tecnologia (Portugal)Banco SantanderUniversidad Complutense de MadridDepto. de Mineralogía y PetrologíaFac. de Ciencias GeológicasTRUEpu

Whistleblowing, notitia criminis y derecho procesal premial

La persecución y enjuiciamiento de toda conducta criminal exige la concurrencia de una serie de requisitos esenciales. El primero de todos ellos se centra en la capacidad de los Estados de detectar la comisión de tales conductas. Aquellos hechos delictivos que no sean conocidos por parte de las autoridades no llegarán a ser investigados ni podrán ser, en consecuencia, debidamente reprimidos, impidiéndose así que la sanción penal cumpla su función preventiva. Por tanto, lo primero que necesitan las autoridades es obtener y asegurar la mayor cantidad de información relacionada con los hechos presuntamente delictivos. Con tal propósito de obtener esa información, los ordenamientos jurídicos cuentan con distintas vías. La «cultura whistleblowing» va a desempeñar un papel trascendental a la hora de facilitar e incentivar ese aporte de información, pero el ordenamiento procesal cuenta también con importantes premios y alicientes con los que incentivar la colaboración con la Justicia por parte de investigados y acusados.Depto. de Derecho Procesal y Derecho PenalFac. de DerechoTRUEpu

Colecciones de aleaciones

Ediciones ComplutenseFac. de Ciencias QuímicasTRUEpu

Memoria y documentación del proyecto

Comunidad de MadridEdiciones ComplutenseEdiciones ComplutenseTRUEpu

Aulas dinámicas: aprendizaje activo en entornos multiculturales/Dynamic Classrooms: Active Learning in Cross-Cultural Settings (DALCS)

DALCS es un proyecto internacional de innovación docente que investiga, en el contexto de la docencia en inglés, con un estudiantado de diversas procedencias y un entorno multicultural, cómo potenciar el aprendizaje a través de aulas dinámicas y cómo identificar y resolver los problemas asociados a esta metodología creando estrategias específicas de respuesta.Depto. de Lógica y Filosofía TeóricaFac. de FilosofíaFALSEsubmitte

Quantifying the spread in sudden stratospheric warming wave forcing in CMIP6

PRE2020-091812Sudden stratospheric warmings (SSWs) show a large spread across climate models in characteristics such as frequency of occurrence, seasonality and strength. This is reflective of inherent model biases. A well-known source of inter-model variability is the parameterized gravity wave forcing, as the parameterization schemes vary from model to model. This work compares the simulation of boreal SSWs in historical runs for seven high-top Climate Model Intercomparison Project Phase 6 models and in two reanalyses. The analysis is focused on the evolution of the different terms in the transformed Eulerian mean zonal mean zonal momentum equation. A large spread is found between models and with reanalyses in the mean magnitude of the resolved and parameterized wave forcing and the responses (wind deceleration and anomalous residual circulation). The results reveal that, in the stratosphere, both the wind deceleration and the strengthening of the residual circulation during SSWs correlate linearly across the models with anomalies in the resolved wave forcing. In the mesosphere, the forcing is a combination of resolved waves and, predominantly, parameterized gravity waves. Models with larger gravity wave forcing anomalies produce larger changes in the residual circulation, while models with larger resolved wave forcing anomalies produce stronger wind deceleration, which we attribute to differences in the spatial shape of resolved and parameterized wave forcing. Although the forcing–response relation across individual SSW events is similar for each model in the stratosphere, this does not hold in the mesosphere. Our results are useful for interpreting the spread in projections of the dynamical forcing of SSWs in a changing climate.Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionDepto. de Física de la Tierra y AstrofísicaFac. de Ciencias FísicasInstituto de Geociencias (IGEO)TRUEpu

Robust large area molecular junctions of selfassembled monolayers of a model helical paddlewheel complex

The author acknowledge support from the Universidad Complutense de Madrid (GRFN32/23, GRFN24/24 and project PR3/23-30828), the France 2030 government investment plan managed by the French National Research Agency under grant reference PEPR SPIN – SPINMAT ANR-22-EXSP-0007, the Imperial College – CNRS joint PhD program, Quantum Matter Bordeaux, the MaelStroM project (CNRS MITI program), the National Research Agency project AnaCrU-CISS (ANR-23-CE09-0026) and the GPR Light project LIGHT-057-ChiroSurf. I.C. acknowledges predoctoral grant from the Complutense University of Madrid and Banco Santander (CT82/20-CT83/20). The authors warmly thank J.P. Salvetat of the Placamat service unit (France) for the ToF-SIMS measurements and Stéphane Toulin for assistance with the open data repository. Computer time for the theoretical calculation was provided by the computing facilities MCIA (Mésocentre de Calcul Intensif Aquitain) of the Université de Bordeaux.We report the preparation of a helical complex and its study in molecular junctions. We show that the SAMs of this racemic compound present an electrically robust behaviour which will pave the way for future studies of the CISS effect with analogous enantiopure compounds.Universidad Complutense de MadridAgence Nationale de la Recherche (France)Imperial CollegeBanco SantanderUniversité de BordeauxDepto. de Química InorgánicaFac. de Ciencias QuímicasTRUEpu

Hacia una teoría de la educación para el bienestar

Ediciones ComplutenseEdiciones ComplutenseTRUEpu