Direktno kompresibilni koprocesovani ekscipijens dobijen sušenjem raspršivanjem pogodan za formulaciju tableta sa visoko doziranom lekovitom supstancom

Novo tehničko rešenje primenjeno na nacionalnom nivou (M82

Intravenous nanoemulsions loaded with CW-02-79-phospholipid complex for enhanced brain delivery: physicochemical, in vitro and in vivo performances

This study addresses the challenge of poor solubility of novel psychopharmacological drug candidates (L/DCs) that hinders their development. ..

Comprehensive Phytochemical Analysis and Evaluation of Antioxidant, Antimicrobial, Cytotoxic, and Immunomodulatory Activities of Commercial Cinnamon Bark Essential Oil (Cinnamomum zeylanicum L.)

The essential oil derived from the bark of Cinnamomum zeylanicum L., Lauraceae, has gained significant attention because of its numerous biological benefits. This study aimed to perform a phytochemical analysis of commercially available Cinnamomum zeylanicum bark essential oil and to evaluate its antioxidant, antimicrobial, immunomodulatory, and antitumor properties. GC–MS analysis was employed to determine the phytochemical composition. The major component of the total essential oil composition was (E)-cinnamaldehyde, constituting 77.93%, followed by eugenol (4.34%), E-caryophyllene (3.68%), and linalool (2.79%). The antioxidant activity was confirmed by DPPH, ABTS, CUPRAC, and TAC assays. In the broth microdilution assay, cinnamon essential oil demonstrated strong antimicrobial activity, with MIC values ranging from 7.37 to 29.50 µg/mL. Furthermore, cinnamon essential oil demonstrated selective antitumor activity by inducing apoptosis and cell-cycle arrest in human colorectal cancer cells (HCT116) while sparing non-cancerous cells (MRC-5). In HCT116 cells, cinnamon essential oil induced apoptosis, downregulated Cyclin D and p-AKT, and caused G1-phase arrest. Additionally, cinnamon essential oil modulated immune responses by reducing pro-inflammatory cytokine production in activated splenocytes and enhancing pro-inflammatory activity in naïve cells. These findings highlight the great potential of the cinnamon bark essential oil in the development of new therapeutic agents

Implementacija vakcinacije u apotekama protiv humanog papiloma virusa: Pregled literature

In most EU countries, pharmacists as community-based health care professionals (HCPs) can provide education and vaccination services, which can enhance convenience and accessibility for patients. This is especially important in areas where vaccine-coverage rates (VCRs) are suboptimal and in underserved areas where access to HCPs may be limited. The aim of this review is to describe the impact and feasibility of human papillomavirus (HPV)-related pharmacy-based vaccination (PBV) programs. The findings indicate that expanding access to vaccinations through pharmacies has the potential to improve immunization rates, particularly in underserved or hard-to-reach populations. Pharmacists’ roles as advocates, educators, and vaccine providers can address barriers, improve awareness, and promote vaccination uptake. Collaboration with other HCPs is essential for enabling a comprehensive approach to HPV vaccination promotion. In conclusion, PBV programs have the potential to serve as an effective strategy for improving immunization rates in both rural and urban areas. The integration of pharmacies, through multi-disciplinary approach into HPV immunization programs can expand access, spread awareness, provide education, clarify concerns, increase convenience, and reach underserved populations. However, careful planning, training, and collaboration with other HCPs such as physicians and nurses, are necessary to overcome challenges and ensure the safe and efficient delivery of vaccines through pharmacies.U većini zemalja EU, farmaceuti kao zdravstveni radnici u zajednici mogu pružati obrazovne i vakcinalne usluge, što može poboljšati pogodnost i dostupnost za pacijente. Ovo je posebno važno u oblastima gde su stope vakcinacije (VCR) suboptimalne i u nedovoljno opskrbljenim područjima gde je pristup zdravstvenim radnicima (HCP) ograničen. Cilj ovog pregleda je da opiše uticaj i izvodljivost programa vakcinacije vezanih za humani papiloma virus (HPV) koji se sprovode u apotekama. Nalazi ukazuju na to da širenje pristupa vakcinama putem apoteka ima potencijal da poboljša stope imunizacije, posebno u nedovoljno opskrbljenim ili teško dostupnim populacijama. Uloge farmaceuta kao pobornika, edukatora i pružalaca usluge vakcinacije mogu prevazići prepreke, poboljšati svest i podstaći prihvatanje vakcina. Saradnja sa drugim zdravstvenim radnicima je ključna za omogućavanje sveobuhvatnog pristupa promociji HPV vakcinacije. Zaključak je da programi vakcinacije u apotekama imaju potencijal da budu efikasna strategija za poboljšanje stopa imunizacije u ruralnim i urbanim oblastima. Integracija apoteka, kroz multidisciplinarni pristup u programe imunizacije protiv HPV-a, može proširiti pristup, povećati svest, pružiti obrazovanje, razjasniti zabrinutosti, povećati pogodnost i dostići nedovoljno opskrbljene populacije. Međutim, pažljivo planiranje, obuka i saradnja sa drugim zdravstvenim radnicima kao što su lekari i medicinske sestre su neophodni da se prevaziđu izazovi i obezbedi sigurna i efikasna isporuka vakcina putem apoteka

From Chemistry to Pharmacology: Exploring the Anti-Inflammatory and Antioxidant Potential of Novel Dexketoprofen Amide Derivatives

In the present study, five novel dexketoprofen amide derivatives with a free carboxyl group in their side chains were synthesized. The in vivo anti-inflammatory potential of dexketoprofen derivatives was evaluated using a carrageenan-induced paw edema model of acute inflammation. Additionally, the local and systemic redox status in rats following acute administration of the compounds was assessed by measuring levels of pro-oxidative markers and the activity of antioxidant enzymes. Among the analyzed molecules, derivatives 2 and 4 exhibited the most potent in vivo anti-inflammatory activity, showing effects comparable to those of the parent compound dexketoprofen. In vitro results revealed that all newly synthesized compounds exhibited low inhibitory activity toward COX-1, whereas only compound 4 showed significant COX-2 inhibition. The stronger binding affinity of derivative 4 for COX-2 in comparison to other tested compounds is likely attributed to its ability to form multiple electrostatic interactions within the enzyme’s active site. Furthermore, compounds 2 and 5 demonstrated efficacy comparable to the parent drug in restoring redox balance, indicating their potential antioxidant properties under acute inflammatory conditions. The findings of this study underscore the therapeutic potential of the novel dexketoprofen amide derivatives as dual-function agents with the capacity to modulate both inflammatory responses and oxidative stress

Istraživanje antropometrijskih, biohemijskih i nutritivnih podataka kod gojaznih i žena sa prekomernom težinom: uvid iz srpske kohorte – pilot studija

Background: Anthropometric measurements (AMs), such as waist circumference and body mass index (BMI), are dominant indicators of overweight and obesity. Nevertheless, other AMs, such as waist-hip ratio, waist circumference and indices of visceral adiposity index (VAI), body mass fat index (BMFI), cardiometabolic index (CMI) and waist-to-height ratio (WtHR) are more significant and discriminatory than BMI in predicting cardiometabolic risk. Methods: This pilot study aimed to comprehensively investigate the anthropometric, biochemical and nutritional characteristics of a sample of overweight and obese women in Serbia, focusing on potential differences related to AM and its association with specific biochemical markers. Results: The final analytical sample consisted of 57 females (average age 37.16±7.27 years, range: 21–55 years). A strong positive correlation was observed between triglycerides (TG) and both the VAI (r=0.896, p<0.001) and the CMI (r=0.896, p<0.001), both with high statistical significance. Conversely, a strong negative correlation was found between high-density lipoprotein cholesterol (HDLc) and both VAI (r=-0.601, p<0.001) and CMI (r=-0.566, p<0.001). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were significantly positively correlated with both WtHR and BMFI: SBP and WtHR (r=0.343, p=0.009), SBP and BMFI (r=0.310, p=0.019), DBP and WtHR (r=0.368, p=0.005), and DBP and BMFI (r=0.377, p=0.004). The analysis of biochemical parameters indicated no significant differences between groups, except for TG levels, which were significantly higher in the obese group (p=0.045). Conclusions: Although the AMs of the subjects were significantly different, energy intake and macronutrient intake were not significantly different. We did not find significant differences in the intake of most vitamins or minerals between the groups. Additionally, there is inadequate intake of essential nutrients such as vitamin D, iron and selenium, which are critical for overall health. Both groups did not meet the recommended folate intake, which may increase the risk of fetal neural tube defects.Uvod: Antropometrijska merenja (AM), kao što su obim struka i indeks telesne mase (BMI), predstavljaju dominantne pokazatelje prekomerne težine i gojaznosti. Ipak, druga AM, kao što su odnos struka i kukova, obim struka i indeksi visceralnog indeksa adipoznosti (VAI), indeks telesne masti (BMFI), kardiometabolički indeks (CMI) i odnos struka prema visini (WtHR), pokazuju veći značaj i diskriminativnu vrednost od BMI-a u predikciji kardiometaboličkog rizika. Metode: Ova pilot studija je imala za cilj sveobuhvatno istraživanje antropometrijskih, biohemijskih i nutricionih karakteristika na uzorku žena sa prekomernom težinom i gojaznošću u Srbiji, sa fokusom na potencijalne razlike povezane sa antropometrijskim merenjima i njihovom povezanošću sa specifičnim biohemijskim markerima. Rezultati: Konačan analitički uzorak je činilo 57 žena (prosečna starost 37,16±7,27 godina, raspon: 21–55 godina). Zabeležena je snažna pozitivna korelacija između triglicerida (TG) i VAI (r=0,896, p<0,001) i CMI (r=0,896, p<0,001), obe sa visokom statističkom značajnošću. Nasuprot tome, pronađena je snažna negativna korelacija između holesterola lipoproteina visoke gustine (HDLc) i VAI (r=-0,601, p<0,001) i CMI (r=-0,566, p<0,001). Sistolni krvni pritisak (SBP) i dijastolni krvni pritisak (DBP) su bili značajno pozitivno korelisani sa WtHR i BMFI: SBP i WtHR (r=0,343, p=0,009), SBP i BMFI (r=0,310, p=0,019), DBP i WtHR (r=0,368, p=0,005) i DBP i BMFI (r=0,377, p=0,004). Analiza biohemijskih parametara nije pokazala značajne razlike između grupa, osim u nivou TG, koji je bio značajno vići u grupi gojaznih (p=0,045). Zaključak: Iako su AM vrednosti ispitanica bile značajno različite, unos energije i makronutrijenata nije se značajno razlikovao. Nismo pronašli značajne razlike u unosu većine vitamina ili minerala između grupa. Pored toga, zabeležen je nedovoljan unos esencijalnih nutrijenata kao što su vitamin D, gvožđe i selen, koji su ključni za opšte zdravlje. Obe grupe nisu dostigle preporučeni unos folata, što može povećati rizik od defekata neuralne cevi kod fetusa

Sustainability by design – A new paradigm for drug product life-cycle management

The essential principle of sustainable development is to “meet the needs of the present without compromising the ability of future generations to meet their own needs” [1]. In order to achieve this, a paradigm shift is necessary in the way we think and act as citizens and professionals. Scientists in different disciplines are expected to integrate sustainability principles in their work, investigate topics associated with sustainable development and provide solutions for sustainability challenges [2, 3]. The Sustainability by Design (SbD) in pharmaceutical sciences emphasizes the integration of sustainability principles across the entire drug product life cycle. In this work, key dimensions of SbD in different stages of pharmaceutical product life cycle, relevant tools and points of action are reviewed: • Research and development based on: (i) predictive modelling of pharmaceutical safety and efficacy, (ii) green chemistry principles, (iii) patient-centric product design, and (iv) early environmental impact assessment. • Manufacturing and distribution incorporating the principles of ecological footprint minimization through employment of: (i) renewable resources and waste minimization, (ii) biodegradable, recyclable active pharmaceutical ingredients, excipients and packaging materials, and (iii) process digitalization. • Clinical use guided by: (i) rational prescribing; (ii) precision dosing, (iii) enhanced adherence, and (iv) incorporation of digital health tools, leading to therapy optimisation and improved health outcomes, while avoiding pharmaceuticals overuse and reducing waste. SbD paradigm in pharmaceutical sciences is an advanced, interdisciplinary concept which extends beyond the laboratory benchtop and manufacturing site to align pharmaceutical innovation with the broader objectives of environmental stewardship, pharmacoeconomic efficiency, and improved patient health outcomes.Koncept održivog razvoja zasniva se na principu da “se potrebe sadašnjeg društva zadovolje bez ugrožavanja mogućnosti budućih generacija da ostvare svoje potrebe” [1]. Ostvarivanje ovog principa zahteva promenu načina razmišljanja i postupanja, kako na ličnom, tako i na profesionalnom planu. Od istraživača u svim oblastima nauke očekuje se integracija principa održivosti u naučni i stručni rad, proučavanje tema povezanih sa održivim razvojem i formulisanje rešenja usmerenih ka prevazilaženju izazova u postizanju održivosti [2, 3]. Paradigma “Održivost kroz dizajn” u farmaceutskim naukama podrazumeva integraciju principa održivog razvoja u sve faze životnog ciklusa leka. U ovom radu razmatrane su ključne dimenzije koncepta “Održivost kroz dizajn” u različitim fazama životnog ciklusa leka, odgovarajući alati i aktivnosti: • Istraživanje i razvoj zasnovani na: (i) prediktivnom modelovanju efikasnosti i bezbednosti, (ii) primeni principa zelene hemije, (iii) dizajnu proizvoda usmerenom na pacijenta, (iv) ranu procenu uticaja na životnu sredinu. • Proizvodnja i distribucija sa minimizovanim uticajem na životnu sredinu kroz: (i) upotrebu obnovljivih resursa i smanjenje otpada, (ii) primenu biorazgradivih aktivnih farmaceutskih supstanci i ekscipijenasa, kao i reciklabilnih materijala za pakovanje, (iii) digitalizaciju procesa. • Klinička primena koja podrazumeva: (i) racionalno propisivanje lekova, (ii) precizno doziranje, (iii) unapređenje adherence i (iv) integraciju digitalnih alata, što omogućava optimizaciju terapije i poboljšanje zdravstvenih ishoda uz izbegavanje prekomerne upotrebe lekova, kao i smanjenje otpada. Paradigma “Održivost kroz dizajn” u farmaceutskim naukama predstavlja savremen, interdisciplinarni koncept koji prevazilazi okvire laboratorijskih istraživanja i tradicionalnih proizvodnih procesa, sa ciljem da se inovacije usklade sa širim prioritetima očuvanja životne sredine, farmakoekonomske efikasnosti i unapređenih zdravstvenih ishoda pacijenata

TechNote - Minimum Preanalytical Information for the Publication of Studies on Circulating Cell-Free microRNA-based Biomarkers

Circulating cell-free microRNAs (miRNAs) are emerging as promising biomarkers with broad potential for clinical applications. However, pre-analytical variability significantly affects miRNA quantification and hampers reproduc- ibility across studies. This work aims to define the Minimum Preanalytical Information required for the publication of studies on circulating cell-free miRNA-based biomarkers. We review key pre-analytical factors that influence circu- lating miRNA levels quantified using RT-qPCR. Critical variables include blood collection timing, sample type, centrifugation protocols, transport and storage conditions, hemolysis, lipemia, medication, physical activity and pathogen inactivation methods. We introduce a standardized checklist to promote methodological transparency and inter-study comparability. The final aim is to enhance the reliability of miRNA-based biomarker research and support its successful translation into clinical practice

Progress toward Implementing Multiomic Approaches in Atherosclerotic Cardiovascular Disease: Update from the 4 th AtheroNET Meeting in Sarajevo (Bosnia and Herzegovina)

The COST Action CA21153 “Network for Implementing Multiomic Approaches in Atherosclerotic Cardiovascular Disease Prevention and Research” (AtheroNET; https://atheronet.eu) was launched in October 2022 with the mission to accelerate the application of multiomics in atherosclerosis research and to foster collaboration among experts from diverse disciplines. Born from the pressing need to advance atherosclerosis management and overcome existing challenges, AtheroNET creates a collaborative arena where innovation thrives through dialogue. The initiative was spearheaded by Prof. Miron Sopić (Faculty of Pharmacy, University of Belgrade), the primary proposer of the Action. The Action is led by Chair Prof. Paolo Magni (Università degli Studi di Milano) and Vice-Chair Prof. Yvan Devaux (Luxembourg Institute of Health), with strategic support from a dedicated leadership team: • Grant Holder Scientific Representative: Dr. Ines Potočnjak • Science Communication Coordinator: Prof. Georgios Kararigas • Grant Awarding Coordinator: Dr. Susana Novella • WG1 - Pathophysiological mechanisms: applying multiomics to uncover novel pathogenic players and processes; Leader: Prof. Dimitris Kardassis • WG2 - Personalized clinical models: translating omics insights into improved management of ASCVD; Leader: Prof. Alberico Catapano • WG3 - Standardization and harmonization: developing SOPs and guidelines to enhance reproducibility across omics research; Leader: Dr. Marie Mardal • WG4 - Data integration and ML/AI: optimizing algorithms for integrating complex multiomic datasets; Leader: Dr. Aleksandra Gruca • WG5 - Dissemination and communication: sharing advances with scientists, clinicians, patients, and the public; Leader: Prof. Georgios Kararigas Since its inception, AtheroNET has grown to 475 members from across Europe, embracing COST’s core values of inclusiveness and excellence across geography, gender, and career stage. A significant proportion of members come from Inclusive Target Countries (n=282), with strong representation of women (n=277) and early-career researchers (n=223)

Low-energy nanoemulsions with citrus essential oils: formulation development and antioxidant activity

The main goal of this study was to develop stable low energy nanoemulsions with 6 different citrus essential oils as potential actives in cosmetic (nano)formulations and to evaluate their antioxidant activity