Antimicrobial Resistance and Molecular Identification of Clinical Multi-Drug Resistant Enterobacter Cloacae

Enterobacter cloacae is a Gram-negative bacteria causing nosocomial infections. This bacteria has increased resistance to various antibiotics in the past five years, resulting in a multi-drug-resistant (MDR) phenotype. In particular, MDR E. cloacae causes longer hospitalization time, increases medical costs, and affects morbidity and mortality. This study aimed to observe the minimum inhibitory concentration (MIC) of clinical E. cloacae towards several antibiotics and molecular identification of MDR E.cloacae. This study was conducted in a descriptive design. Secondary data was collected at the microbiology laboratory of the Teaching Hospital in Bekasi, Indonesia, from May to September 2020. Sampel was carbapenem resistant E.cloacae. The isolate was originated from a human clinical specimen, then was confirmed molecular identification using 16s rRNA. In this study, only one carbapenem-resistant E. cloacae, which is also MDR bacteria, was found. This E. cloacae was categorized as MDR bacteria since it was resistant to more than three antibiotic classes, including carbanemen, extended-spectrum cephalosporin, penicillins + β lactamase inhibitor, antipseudomonal penicillins + β lactamase inhibitor aminoglycoside, and penicillin. Vitek 2 identification of this isolate was E. cloacae complex. It showed similar results to molecular identification based on a partial sequence of 16s rRNA. BLASTn result of the trimmed sequence was E. cloacae with 99.78 % similarity

Polyploidy Induction of Rutaceae through Bio-catharanthine Treatment

Indonesia has a tropical monsoon-type climate which suitable for agriculture and plantations. One of the main centers of orange fruits (Citrus sp.) production in Indonesia and become the top ten with the largest production is in South Sulawesi. Farmers commonly cultivate siam oranges (Citrus nobilis). Lime (Citrus aurantifolia) has not yet become the main production, which is inversely proportional to the high demand in South Sulawesi. This study aims to determine the ability of bio-catharanthine as a polyploidy agent against the phenotype of the familia Rutaceae cultivated in South Sulawesi. The research was carried out in Laboratory of Genetics and Molecular Biology, Department of Biology, Universitas Islam Negeri Alauddin Makassar using randomized complete block design consisted of two conditions (bio-catharanthine concentration including C1 = Bio-catharanthine 0.05%; C2 = Bio-catharanthine 0.075%; C3 = Bio-catharanthine 0.1% and immersion time including T1 = 3 h; T2 = 6 h). Phenotypic observations include plant height, the number of leaves and nodes, the length of roots, and the number of roots per shoot. The results of the study showed that the bio-catharanthine immersion of 0.05-0.075% for 6 hours in Rutaceae had a significant effect on their phenotypic characters

pengaruh kombinasi ekstrak benalu teh dan mangga terhadap profil histopatologi tikus hipertensi (DOCA-SALT)

The purpose of this study was to analyze the brain histopathological profile in hypertensive rats (DOCA-Salt) treated to Tea and Mango extract combination (Mistletoe). Samples were obtained using a true experimental design completely randomized on 25 rats with three treatments (TI, TII, TIII), negative control, and positive control with replication five times. Ratio of extract for treatment was 3 : 1 made up 50 mg/kg BW, 100 mg/kg BW, 200 mg/kg BW. Data analysis using one-way analysis of variance (ANOVA) with the JAMOVI application. The study results obtained Mistletoe (Tea and Mango) extract combination in hypertensive rats (DOCA-Salt) can significantly reduce the necrosis of brain cells in the white matter area. The combination of Mistletoe extract can reduce the number of brain cell necrosis in hypertensive rats optimally obtained at a dose of 50 mg/kg B

The Ethnobotany Study of Medicinal Plants in Lombok Island

Lombok has the biodiversity plant potential that can be used as medicine. This study aimed to identify the types of medicinal plants used by Sasak people, how to obtain, the parts used, how to use and processing, and the Species Use Value (SUV), Family Use Value (FUV), and Fidelity Level (FL). The results showed that there were 84 species used as traditional medicine by Lombok people. The method of obtaining medicinal plants was mostly done by cultivation, as much as 58% of the processing methods, and the most common use of plants in medicine was boiling and drinking. Plant species that had the highest SUV value were Morinda citrofolia, Zingiber officinale, and Elephantopus scaber with SUVs of 0.8 each. The plant family that had the highest FUV value was Zingiberaceae (eight species), furthermore, the part of the plant that had the highest value was the leaf. The total of plant species that had the highest Fidelity level was 52. One of them was celery (Apium graveolens L.). The data obtained from this research was needed to create an inventory of medicinal plant species and their use by the community, so that traditional knowledge of medicinal plants could be documented and preserved

Comparison of the Chaetodontidae Mitochondrial Genome

The mitochondrial genome is useful for identification because the mutation rate of mtDNA is higher than nDNA. Previous studies have carried out many analyses on the mitochondrial genome of several species, the Chaetodontidae group. Fish in this group are biological indicator fish of coral reefs, so their molecular characteristics must be enriched to conserve these fish. This article aims to review several forms of the mitochondrial genome of the Chaetodontidae group of species, including: Chaetodon speculum, Chaetodon octofasciatus, Chaetodon wiebeli, and Heniochus chrysostomus. Data were collected qualitatively by reviewing previous research articles related to the Chaetodontidae mitochondrial genome. The review focused on the characteristics of each species' mitochondrial genome, including length, overall composition, tRNA, and non-coding region. Each species exhibits various features of the mitochondrial genome and is mapped in its phylogenetic analysis

Growth and Mortality Rate of Scad (Decapterus macrosoma, Bleeker 1851) landed at Inengo Fish Landing Base, Bone Bolango, Gorontalo

This study aimed to determine the growth pattern and mortality of Scad (Decapterus macrosoma) landed at Inengo Fish Landing Base. A total of 400 fish samples were obtained from the catches of fishers who landed their catch at the landing base using the layered random sampling method. Sampling was carried out from February to March 2021 by measuring the length and weight of the fish samples. Data were analyzed using Microsoft Excel and the FiSAT II application. The results showed that the growth pattern of D. macrosoma was positive allometric with the asymptotic length (L∞)= 279.54 mm, the coefficient of growth rate (K)= 0.49, and the theoretical age of fish (t0)= -0.18 years. The fishing mortality (F) was 2.44 per year; the natural mortality (M) was 0.43 per year; the exploitation (E) was 0.85

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Obesity caused by a high-fat diet leads to an altered reproductive hormonal profile, including impaired semen quality. Antioxidants can overcome these conditions. One of the well-known sources of antioxidants is in the rambutan fruit peel extract. This research aimed to figure the effect of rambutan fruit peel extract towards total sperm count in Wistar rats induced with high-fat feed. The study design used post-test only control group,  subjects were 30 male Wistar rats divided into five groups: Group I (Positive Control) was given high-fat feed, Group II (Treatment Control Group) was given 15mg/kg BW rambutan fruit peel extract, whereas group III, IV, V (Treatment Group) were given an extract of rambutan skin with a dosage of 15, 30, and 60 mg/kg BW, respectively. Treatment was administered for 81 days. This study showed that rambutan fruit peel extract with doses of 15, 30, and 60 mg/kg BW has significantly increased total sperm count in Wistar rats induced with high-fat feed. The outcome using Kruskal Wallis shows a result of p-value 0,010 (CI 95%). Antioxidant compounds found in rambutan fruit peel extract significantly increase total sperm count in Wistar rats induced with high-fat feed, with the highest efficacy on the dose of 15mg/kg BW. &nbsp

In-silico Approach for The Prediction of Chlorogenic Acid as PPAR-γ Activator

Coffee is one of the essential crops commonly cultivated in Indonesia. Coffee contains diverse bioactive compounds, which are associated with human health benefits. One of the compounds is Chlorogenic acid, which able to decrease the risk of type 2 diabetes mellitus (T2DM). However, the mechanism of chlorogenic acid toward anti-diabetes still unclear. This study aimed to analyze and investigate the potential role of chlorogenic acid as anti-diabetes through their interaction with Peroxisome proliferator-activated receptor gamma (PPAR-γ) as an enzyme to phosphorylate and regulate the mechanism of T2DM. The physicochemical properties of chlorogenic acid also performed in this study. The PPAR-γ was downloaded from the PDB database, and the chlorogenic acid was retrieved from the PubChem database. The protein and ligand were prepared using the PyRx program and were docked using Hex 8.0.0 software. Discovery Studio client 4.1 software was used to analyze the interaction between chlorogenic acid and PPAR-γ protein. Based on the physicochemical properties, chlorogenic acid is highly permeable to the cell and easily absorbed.  Thirteen amino acid residues of PPAR-γ (GLN410, SER394, ASP396, GLY395, GLU407, LEUA401, LEU400, VAL403, LYS373, LYS438, LEU377, LYS434, and GLY437) were identified on the chlorogenic acid-PPAR-γ interaction. Interestingly, the kind of interactions, including hydrophobic interaction, hydrogen bond, and van der Waals, which are supported by the tight interaction. Our study indicated that chlorogenic acid might have anti-diabetes activity through PPAR-γ interaction. &nbsp

Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC

Biolarvacide of Culex quinquefasciatus

Biolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus. &nbsp