Foot Print of Macro Fungi in The Coastal Forest of Bama, Baluran National Park, East Java

Baluran National Park, West Java, as one of the conservation sites in Indonesia, has the attraction of the varied types of ecosystems, including fungi. This study aimed to analyze the diversity of fungi in Bama Coastal Forest, Baluran National Park. The method was explorative with plot purposive sampling technique. Parameters in this study include abundance, dominance, and diversity of fungi enriched with physical parameters of humidity and temperature. The fungi were documented and macroscopically observed. Data were analyzed using the abundance index, dominance index, and diversity index. This research identified 18 types of macrofungi in Bama Coastal forest, Baluran National Park East Java including Ganoderma, sp, Hexagonia tenuis, Trametes hirsute, Phellinus sp.1 and sp.2, Ganoderma applanatum, Phellinus igniarius, Pycnoporus cinnabarinus, Daedalea quercina, Tyromyces chioneus, Microporus xanthopus, Calvatia sp., Irpex lacteus, Trichaptum sp., Lentinus sp. Poria corticola, Tyromyces sp., and Lichemomphalia sp. One fungi species (Ganoderma sp.) has the highest abundance index (27.62). The Medium abundance index was found in three species (Hexagonia tenuis, Tyromyces  chioneus, Tyromyces sp.) while the rest fungi were in low abundance index. Dominance index of all fungi species identified in Bama was in low category (D<0.5) with medium diversity (2.30). It can be concluded that the diversity of microfungi in the coastal forest of Bama, Baluran National park was a distribution medium of individual of each species and medium community stability. Environmental conditions in the Bama Coast Forest with 93% humidity and an average air temperature of 26-27oC could support for the fungi to grow quite well

Stomata Profile Comparisons in Abaxial and Adaxial Zones of Dendrobium aphyllum and Arachnis flos-aeris Leaves

Stomata activity in orchids is related to orchids' adaptation process to their environment. This study aimed to investigate the stomata profile, comprising of anatomical structure and density of the two orchids species, Dendrobium aphyllum, and Arachnis flos-aeris. Data was collected by printing the leaves' surface using glue and observed at 09.00 am and 09.00 pm, then analyzed using Image raster 3.0 software and MS. Excel. The stomata of D. aphyllum and A. flos-aeris were characterized by anomocytic type. Results showed that the highest stomata density was 1177.78 um2, found on the abaxial part of D. aphyllum. The adaxial leaves of both species were classified as low or no stomata. These two orchid species were epiphyte yet grow in different habitats. Arachnis flos-aeris exposed to direct sunlight tends to reduce transpiration rate, resulting in fewer stomata than D. aphyllum less exposed to sunlight

Rheotaxis Response Based on Sexual Dimorphism in the Green Swordtail Fish, Xiphophorus hellerii

Morphological distinctions between males and females of a species are referred to by sexual dimorphism. It may result from various selection pressures affecting either sex or both and may occur in any dioecious species, including Green Swordtail fish, which are sexually reproductive. This study examined the different rheotaxis responses of Xiphophorus hellerii based on different sexes and morphological features. We analyzed ten adult males, ten gravid females, and ten non-gravid females of Xiphophorus helleri collected down the river and transferred into the column. We counted the number of the individual that performed positive rheotaxis (+), negative rheotaxis (-), and indifference response (0). The result showed different rheotaxis responses shown by male, non-gravid female, and gravid female X. hellerii. The highest percentage of positive rheotaxis response (movement against the current) was shown by non-gravid female X. hellerii, reaching up to 89%. Morphological differences between male, non-gravid female, and gravid female X. hellerii appear to affect the orientation and ability of X. hellerii in giving response against current and certainly has an impact on their survival in nature

Deteksi Awal Aktivitas Antibakteri dari bakteri air tambak terhadap bakteri patogen akuakultur

Aquaculture is currently experiencing massive loss due to the outbreak of pathogenic bacteria. One of the outbreak causes is the development of pathogenic bacterial resistance to the antibacterial. The problem can be solved using microorganisms that can produce new antibacterial compounds. The purpose of this research was to obtain bacteria from fishpond water that could produce antibacterial compounds. About two out of 81 isolates could produce antibacterial compounds. Those two isolates were obtained from saltwater fishponds in North Jakarta (TS2) and Harapan Island (PHY). All fishpond water was grown in marine broth or Luria broth. Extraction of antibacterial compounds was performed using four types of solvents: chloroform, dichloromethane, ethyl acetate, and methanol. Each of the solvents showed a different result. The extraction can only be successfully performed using chloroform and dichloromethane. Extraction using dichloromethane showed a larger inhibitory clear zone than chloroform. Based on 16S rRNA gene sequencing, PHY isolate was identified as Bacillus sp. and TS2 as Acinetobacter sp. In conclusion, isolate TS2 and PHY, which produced antibacterial compounds, showed potential use as aquaculture probiotics

Diversity, Abundance, and Distribution Patterns of Natural Enemy Insects on Chili (Capsicum annum L.) in Bincau Village, Banjar Regency, South Kalimantan

Chili is a widely cultivated plant by the community because of its profit. Currently, in maintaining the quality of chili, people commonly use a lot of pesticides. In contrast, naturally, there are biological agents in the shape of natural enemy insects, more effective, and environmentally friendly. This study aims to determine the diversity, abundance, and distribution patterns of natural enemy insects in chili plantation (Capsicum annum L.) at Bincau Village, Martapura District, Banjar Regency, South Kalimantan. The sample is collected by pitfall trap, yellow pan trap, and sweep net method. Obtained data are analyzed by Shannon Wienner index (H'), Meyer abundance index, Morisita index (Id), and PCA method for environmental parameter analysis. The results showed the diversity of natural enemy insects on chili plants was moderate (H' = 2.396). Furthermore, the highest abundance in research is demonstrated on Hymenoptera order, which consisted of 9 families, 16 species, and 1,009 individuals. The distribution pattern of natural enemy insects in chili plantations is clustered (Id > 1). Based on the PCA analysis method, the temperature has the most impact on natural enemy insects' presence in this study

Identification of Edible Macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia

Indonesia is considered as a mega-biodiversity country that has a massive amount of vascular and non-vascular plants. The tropical environment condition of Indonesia could support the growth of macrofungi. Information about edible macrofungi from the Forest of  Lombok Island is based on limited data. This research aims to characterize the edible macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia. This research was a descriptive and explorative study. The edible mushrooms were observed through the Cruise method by following the particular track inside the forest. The sample found in the forest then documented and evaluated. A morphological analysis procedure was performed to assess the profile and similarity between the microscopic evaluations with the mushrooms identification book. In this study, we also offered a phylogeny analysis based on morphological characters similarity. The Dendogram tree was reconstructed using PAST 3.0. software. The result showed that there are eight species of edible mushrooms found that were group into Basidiomycota, namely, Termitomyces clypeatus, Termitomyces umkowaan, Termitomyces sp.1, Pleorotus flabelatus, Pleurotus ostreatus, Coprinus desimenatus, Tremella fuciformis, and Polyporus sp. All of them involved in Basidiomycota. Based on characterization and identification, the species are grouped into three Ordos and five Families. Most of the edible macrofungi found at Kerandangan Protected Forest & Natural Park growth at soil and deadwood. &nbsp

The Ability of Soil Candida albicans Secreted Potential Protease and Lipase

Candida spp secreted a different kind of extracellular enzymes. Protease and lipase are the enzymes that are commonly used in the food and pharmaceutical industries. This study aimed to examine protease and lipase activity of Candida tropicalis isolated from the soil of the Medicinal Plant Garden of the Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Indonesia. Candida isolate was subjected to the fermentation process to obtain bioactive metabolites. The result was analyzed using ANOVA within a 5% interval of confidence, continuing with PostHoc. The result showed the Candida tropicalis metabolite giving the best proteolytic index value (0,6556 ± 0,0090) U/mL. The metabolite isolate of Candida tropicalis had the highest activity, amounting to 5,776 ± 0,495 U/mL. The best results of lipolytic index value (0,394 ± 0,053) U/mL.  The Candida tropicalis metabolites produced the highest lipase enzyme after nine days of fermentation in 5.2917±0,0167 U/mL

Cytotoxicity Assay of 2,4-Dihydroxide-4’-Methoxychalcone Against Cervical (HeLa) Cancer Cell by MTT Assay

Chalcone is one of the phenolic group secondary metabolic with numerous biological activity. Many studies have shown that chalcone derivatives compound has anticancer, anti-inflammatory, antimalarial, and antibacterial activities. The purpose of this research was to study the prediction potency unsaturated carbonyl system of chalcone derivative against the HeLa cell by MTT assay. Those activities assumed can inhibit the mechanism action of NF-kB that caused cervical cancer. The 2,4-dihydroxide-4’-methoxychalcone has done synthesis as a target compound by a sonochemical for 7 hours. The results showed that chalcone derivative most active against the HeLa cell. &nbsp

The Growth Comparison of Haematococcus Pluvialis in Two Different Medium

Microalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on  H. pluvialis biomass is exploring its high-value bio compound.&nbsp

Sintesis dan Optimisasi Produksi Kitosan Asal Cendawan Endofit Akar Cagar Alam ulau Dua Banten

The cell wall component of root endophytic fungi is a main source of chitosan synthesis. This research aimed to optimize chitosan synthesis from three different isolated species of root endophytic fungi in Pulau Dua Nature Reserve, Banten Indonesia. Three species of root endophytic fungi, namely, Aspergillus niger, Aureobasidium sp., Basipetospora sp., were cultured to produce chitosan. To optimize the production of fungal chitosan, cultures were treated with different conditions such as growth medium (Oat Meal broth, Potato Dextrose Broth, CMMY Broth), pH value (4, 5, 6, 7), and temperature (25, 30, 35, 40°C). Chitosan was extracted from dried mycelium after treated on NaOH 10% at 121°C for 2 min. The degree of deacetylation (DD) of chitosan was then examined and compared with the control (shrimp chitosan). The optimum fungal chitosan condition was higher in CMMY medium, pH 5, and 30°C. The DD value of three root endophytic fungi species, Aspergillus niger, Aureobasidium sp., Basipetospora sp. were 88.5, 83.3, 80.8, respectively, which was lower than DD value resulted from control. This study indicates that Aspergillus niger was a potential endophytic fungi for chitosan synthesis