Measurements of liquid-liquid phase transition temperatures (cloud points) of
mixtures of a protein (lysozyme) and a polymer, poly(ethylene glycol) (PEG)
show that the addition of low molecular weight PEG stabilizes the mixture
whereas high molecular weight PEG was destabilizing. We demonstrate that this
behavior is inconsistent with an entropic depletion interaction between
lysozyme and PEG and suggest that an energetic attraction between lysozyme and
PEG is responsible. In order to independently characterize the lysozyme/PEG
interactions, light scattering experiments on the same mixtures were performed
to measure second and third virial coefficients. These measurements indicate
that PEG induces repulsion between lysozyme molecules, contrary to the
depletion prediction. Furthermore, it is shown that third virial terms must be
included in the mixture's free energy in order to qualitatively capture our
cloud point and light scattering data. The light scattering results were
consistent with the cloud point measurements and indicate that attractions do
exist between lysozyme and PEG.Comment: 5 pages, 2 figures, 1 tabl