通过提纯的RuBP羧化酶制备兔抗RuBP羧化酶抗体,以辣根过氧化物酶进行酶联标记,对典型的C4植物甘蔗、C3植物水稻和巴西橡胶三品系叶切片进行RuBP羧化酶的定位.通过显微及超微观察,结果表明:C4和C3植物叶切片中RuBP羧化酶的分布明显不同,C4植物的特异颗粒颜色反应(棕色)存在于维管束鞘细胞;C3植物的特异颗粒颜色反应(棕色)存在于叶肉细胞.巴西橡胶IAN873、RRIM600品系叶片内RuBP羧化酶在鞘细胞和叶肉细胞的叶绿体中均有特异颗粒颜色反应(棕色);而天任31 45品系只有叶肉细胞的叶绿体中有特异颗粒颜色反应(棕色).试验还表明,叶肉细胞的RuBP羧化酶发育可能在前,鞘细胞RuBP羧化酶发育可能在后.A rabbit monoclonal antibody raised against ribulose-1,5-bisphosphate carboxylase (RuBPCase) was produced, and RuBPCase was isolated and purified from Hevea brasiliensis and tobacco, the antibody was marked with horseradish peroxidase, RuBPCase in leaf blade transection of typical C_3 plant (rice) and C_4 plant (sugarcane) and H.brasiliensis was located by direct immunoenzyme labeling method. The localization was observed in detail by microscope and electronic ultrascope. The results were as follows: there was difference in the localization of RuBPCase between typical C_3 and C_4 plants, the specific granulated color response (brown) of C_3 plant (rice) only existed in mesophyllous cell of chloroplasts, while the specific granulated color response (brown) of C_4 plant(sugarcane) was only found to be in bundle sheath cell of chlorophasts; The specific granulated color response (brown) of IAN873, RRIM600 of H.brasiliensis were located in both mesophyllous cell and bundle sheath cell of chloroplasts, while the specific granulated color response of Tianren 31-45 was only present in mesophyllous cells. It has been explained by experiment RuBPCase in mesophyllous cells may develop earlier, and RuBPCase in bundle sheath cells may develop later.国家自然科学基金资助项目(39270461)
