用焦锑酸钙沉淀的电镜细胞化学方法 ,研究了低温胁迫下夏威夷椰子 (PritchardiagaudichaudiiH .Wendl.)幼苗叶肉细胞内Ca2 + 水平的变化 .研究结果表明 ,未经低温处理的夏威夷椰子幼苗叶肉细胞 ,焦锑酸钙沉淀颗粒大量出现在液泡中 ,而细胞基质及细胞间隙中则看不到焦锑酸钙沉淀 .而夏威夷椰子幼苗经过 2℃ ,48h低温处理后 ,细胞基质和细胞膜、液泡膜上焦锑酸钙沉淀增加 ,而液泡中的焦锑酸钙沉淀减少 ,并且超微结构已初步显示出寒害的特征 ,叶绿体外膜部分破损 ,类囊体片层稀疏且排列不规则 .而夏威夷椰子幼苗经过 2℃ ,96h低温处理后 ,Ca2 +大量分布于液泡和细胞基质中 ,细胞间隙中也可见少量斑块状沉淀 ,大部分细胞结构已经严重破损 ,内部结构分辨不清呈现出了遭受严重冷害的症状 .从而揭示了Ca2 + 的区域性分布的变化与植物抗寒性的一些关系The changes of Ca 2+ localization in leaf cells of Pritchardia gaudichaudii H. Wendl. under chilling stress were investigated with calcium antimonate precipitate-electromicroscopic-cytochemical methods. When P. gaudichaudii grew at the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca 2+ localization mainly concentrated within the vacuoles. But when P. gaudichaudii was treated at the temperature of 2 ℃ for 48 hours, the level of Ca 2+ increased in cytoplasm and plasma membrane, but decreased in vacuoles considerably. At the same time, the ultrastructure suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoid was exiguous and unclear. And the chilling time was prolonged to 96 hours, the deposits of calcium antimonate mainly concentrated within the cytoplasm and vacuoles and that there was also some Ca 2+ deposits in intercellular spaces, simultaneity, the ultrastructure of some cells was damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear.国家建设部及厦门市建委资助项目 (无编号
