학위논문 (박사)-- 서울대학교 대학원 : 의과대학 의학과, 2018. 2. 김진희.Purpose: Data from animal experiments suggest that exposure to general anesthetics in early life inhibits neurogenesis and causes long-term memory deficit. Regarding a short operating time and popularity of sevoflurane in pediatric anesthesia, it is important to verify effects of short period exposure to sevoflurane on developing brain.
Methods: We measured the effects of short-term exposure (2 h) to 3%, 6% or 8% sevoflurane, the most commonly used anesthetic, on neural precursor cells derived from human embryonic stem cells, SNUhES32. On days 1, 3, 5 and 7 post-treatment, cell survival, proliferation, apoptosis and differentiation were analyzed.
Results: Treatment with 6% sevoflurane increased cell viability (P = 0.046) and decreased apoptosis (P = 0.014) on day 5, but didnt last on day 7. Survival and apoptosis were not affected by 3% and 8% sevofluranethere was no effect of proliferation at any of the tested concentrations. The differentiation of cells exposed to 6% or 8% sevoflurane decreased on day 1 (P = 0.033 and 0.036 for 6% and 8% sevoflurane, respectively) but was again normalized on days 3–7.
Conclusion: The clinically relevant treatment with sevoflurane for 2 h induces no significant changes of the survival, proliferation, apoptosis and differentiation of human neural precursor cells, although supra-clinical doses of sevoflurane alter human neurogenesis transiently.1. Introduction 1
2. Materials and Methods 3
hESC line 3
Derivation of NPCs from hESCs 3
Sevoflurane treatment 4
Determination of the medium concentration of sevoflurane by gas chromatography 5
Cell viability analysis 5
Proliferation analysis 5
Apoptosis analysis 6
Differentiation analysis 6
Statistical analysis 7
3. Result 8
Concentration of sevoflurane in the medium 8
Effects of sevoflurane on the survival of human NPCs 8
Effects of sevoflurane on the proliferation of human NPCs 9
Effects of sevoflurane on apoptosis by human NPCs 10
Effects of sevoflurane on human NPC differentiation 11
4. Discussion 12
5. References 17Docto
