Bache, Michael

Boisen, Anja

Bosco, Filippo

Brøgger, Anna Line

Chen, Ching-Hsiu

Eugen-Olsen, Jesper

Frøhling, Kasper Bayer

Hwang, Ing-Shou

Hwu, En-Te

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 19, 2017Towards Picomolar Detection with DVD-ROM Optical TechnologyBrøgger, Anna Line; Bosco, Filippo; Bache, Michael; Frøhling, Kasper Bayer; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shou; Boisen, AnjaPublished in:Proceedings of 2013 Nanomechanical Sensing WorkshopPublication date:2013Link back to DTU OrbitCitation (APA):Brøgger, A. L., Bosco, F., Bache, M., Frøhling, K. B., Hwu, E-T., Chen, C-H., ... Boisen, A. (2013). TowardsPicomolar Detection with DVD-ROM Optical Technology. In Proceedings of 2013 Nanomechanical SensingWorkshopProStanAnSolu(suPinflstudthe bacandpotesuPof fromandpicofor TheActD2 shomemreceFigufromusuathe pceedings of 2ford UniverTOWna Line Brøgble urokinAR) is a pammation inies have shoactivation stterial or para cancer [1]ntial clinicaAR concentthe patients. tens (heal the requiremolar. CurrELISA testin membrane ivator Recepand D3) anwn in Figubrane, solptor is likelyre 1: uPAR a the membrally also cleavrotein. 013 Nanomesity, StanfordARDS PICger1, Filipp1DTU na2In3Clinical Rase Plasmrotein whic the humawn that theate of the imsitical infect. This makel biomarker ration is ass suPAR cothy) to hundd sensing ently, a diagg of suPARbound moltor (uPAR) d is presentre 1. Whenuble uPAR to degrade ttached to a ne, it becomed, leaving Dchanical Sens, CAOMOLAR Do G. Bosco1,Eugennotech, Testitute of Phesearch Ceninogen Ach reveals in body. Se suPAR conmune systemions and auts it a gooand triagingociated withncentrationsreds (diseassensitivity inostics kit is. ecule urokinconsists of th on several  uPAR is c (suPAR) - either befocell membranes suPAR. Th2D3 as the iing Worksho       ETECTIO Michael Ba-Olsen3, Ing-chnical Uniysics, Acatre, Copenhtivator Recnformation veral large centration re from both oimmune disd candidate  tool; an ele worse prog in humansed) of picoms in the te on the markase Plasminree domainskinds of celeaved fromis formed. re or quickly e. When cleae domain Dnteresting parp    E.-T. HwuN WITH Dche1, KasperShouh Hwanversity of Ddemia Sinicagen Univ eptor about scale flects viral, eases as a vated nosis  vary olar, ns of et [2] ogen  (D1, lls as  the The  after cleaas tdomurinfluiTheaimrapiintefulfusinROstreallosignfastforcplatmicFigudiscROMInitpicotechAntved 1 is t of A. L Brø, C.-H. Chen,VD-ROM B. Frøhlingg2 and Anjaenmark, Ka, Nankangersity Hospvage from the D1 domaan protein se, blood (bd [1].  Multi Senss at providind - tool for gration of mill these neg multiple mM Optical Pss changes iws high-throificant resu scanning oes on the mform facilitaroliter scale.re 2: The Ds contains the head whichial measuremolar detnology for i-D3 was fugger, F. G. B J. Eugen-Ol OPTICA1, En-Te Hw Boisen1 gs. Lyngby Taipei, Taital, Hvidovhe membranin is cleaveuPARD2-D3 roth plasma or DVD Plag a mainlyprognosticsicro- and neds, we proicrocantileickup Unit (n liquid [4] ughput labelts and the rf the sensoicrocantilevetes high pre VD-ROM opti cantilevers.  detects the dements haveection of detection of nctionalizedosco, M. Bacsen, I.-S. HwL TECHNOu2, Ching-Hs, Denmark iwan re, Denmare - into a twd from D2. emains stabland serum) tform (MUS reliable - b of inflammanotechnolopose a labvers and a cOPU) for de(see Figure 2l-free sensinadial symmrs without ars. Furthermcision fluidcal setup. A PThese are scflection of the been condsuPAR wcantilever d on the canthe, K. B. Frøang and A. BLOGY iu Chen2, Jek o-domain pThis solublee and is presand cerebroE) framewout also cheaatory diseasegies. In ord-on-a-chip dommercial Dtection of su). This techg with statetry enablespplying torore, the rotaic handling MMA microfanned by the  cantilevers.ucted to pith DVD-eflection chailevers and Phling,  oisen sper rotein  two-ent in spinal rk [3] p and s via er to evice VD-rface nique istical  very sional tional at the luidic DVD- ursue ROM nges. DGF ProStanwashavstraposdowmeaconstatconof tthe alsoFiguconcsuPAIn oinvehighantichipdimcheantiprevchipovesignwersoftPBSthe suPThewhecanrefeceedings of 2ford Univer used as re been contegy. From sible to deten to belosurements centration ofistical signcentrations iens to hundplatform is n in terms of re 3: suPAentration of R signal minur pursuit tostigated whest deflecti-D2 antibods (IBM, CHensions 500mistry was body in conious experis we used. r 160 individificant resule lost due toware malfun buffer, 150microfluidicAR solution  result of thre the absotilevers withrence cantil013 Nanomesity, Stanfordeference producted to Figure 3 ict suPAR wiw 10 nMis not yet  suPAR beloificance. Sn the humanreds of picomeeded both isensitivity anR detection5 nM (logplus the PDGF improve theich of two aon of the mies were eac); each con µm x 100optimized ttrast to the ments. As Thus, the exual microcats (Note tha i.a. defectivction). After µl of suPA system at ahad a concene experimenlute deflect the two evers are ilchanical Sens, CAtein. QCM-verify the t can be dth the DVD. The sesufficient tw approx. 2ince the  body (serumolar, furthen terms of dd statistical on cantileot).The differ signal.   required devailable anticrocantilevh immobiliztaining 8 m µm x 1 o ensure a uneven layreference, 5periments wntilevers; reat data from e cantilevers 5 minutes oR solution  flow rate otration of 2ts is summaion signals different anlustrated. It ing Worksho       D measuremfunctionalizeduced that -ROM technnsitivity ofo determin0 nM, neithrelevant su) are in the r optimizatietection rang significancevers down ential signal tection rangeibodies yielders. Anti-D3ed on 9 canticrocantileveµm. The suuniform layer attained i blank cantere carried ossuring statia few cantil, misalignmf calibrationwas injectedf 20 µl/min00 nM.  rized in Figu(in mV) otibodies anis observedp    E.-T. Hwuents ation it is ology  the e the er the PAR range on of e but . to a is the , it is s the  and ilever rs of rface er of n the ilever ut on stical evers ent or  with  into . The re 4, f the d the  that bothnot bindsurfdeflaffediffchathatno antiNewreleFigucanD2 y ThiStra RE[1][2][3][4]A. L Brø, C.-H. Chen, antibodies equivalent toing propertace stress tection of thcted by therences in nge the gene in our expsignificant bodies.  strategies vant concenre 4: Antitilever measuield similar rs work has tegic researcFERENCESM. Thuno, molecular cry172, 2009. Virogates A/www.dsf-muF. G. Bosco,M. H. Jakobslabel-free plLab Chip, vogger, F. G. B J. Eugen-Olshow a simi concludingies to suPAhat has the e cantilevere amount oantibody-srated surfacerimental codifference iwill be emtrations of subody characrements. The esulting deflebeen fundeh. : B. Macho, stal ball”, DS, DK. se.org  E.-T. Hwu, Cen, I.-S. Hwaatform for stl. 11, pp. 241osco, M. Bacsen, I.-S. Hwlar response  that the antiR, since it dominating s. The surfaf captured uPAR cone stress.  Honfiguration n using anployed to rPAR.  terization intwo antibodiection of the cad by the DaJ. Eugen-Olisease Marker.-H. Chen, Sng, A. Boisenatistical bio-m1-2416, 2011.he, K. B. Frøang and A. Bto suPAR. Tbodies have is the geneinfluence oce stress wsuPAR butfiguration wever, we nthere seems ti-D2 or aneach the cl connections anti-D3 andntilevers. nish Councsen, “suPARs, vol. 27, pp. Keller, M. B, “High throuolecular sen hling,  oisen his is equal rated n the ill be  also might otice to be ti-D3 inical   with  anti-il for : The . 157-ache, gh-put sing”, 

Towards Picomolar Detection with DVD-ROM Optical Technology

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Towards Picomolar Detection with DVD-ROM Optical Technology

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