Driessen, A. J. M.

Gottschal, J. C.

Marvin-Sikkema, F. D.

Prins, R. A.

English

Anaerobic eukaryotes are often devoid of mitochondria but contain special organelles separated from the cytosol by a single (in fungi) or a double (in protozoa) membrane. Hydrogenosomes from the anaerobic fungus Neocallimastix sp. L2 are thought to catalyse the enzymic steps in the ATP-yielding metabolism of malate into acetate, H-2 and CO2. Isolated hydrogenosomes contain a Mg2+- or Mn2+-dependent ATPase activity. This activity is involved in the maintenance of a pH gradient across the hydrogenosomal membrane, which renders these organelles alkaline inside. ATPase activity and DELYTApH generation is sensitive to diethylstilboestrol but not to other known ATPase inhibitors. Typical inhibitors of the mitochondrial ADP/ATP translocase, bongkrekic acid and carboxyatractylate reduced the ATPase activity, suggesting the presence of a nucleotide transporter. Under anaerobic conditions hydrogenosomes produced H-2 and acetate from malate. This process was found to be dependent on the external supply of ATP or ADP and succinate, and was blocked by protonophores, diethylstilboestrol, and the inhibitors bongkrekic acid and carboxyatractylate. These results demonstrate that hydrogenosomes of Neocallimastix sp. L2 perform the essential functions required for the generation of metabolic energy from malate. It is suggested that hydrogenosomes are functionally related to mitochondria but lack an outer membrane.</p

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   University of GroningenMetabolic Energy Generation In Hydrogenosomes Of The Anaerobic Fungus Neocallimastix -Evidence For A Functional-relationship With MitochondriaMarvin-Sikkema, F. D.; Driessen, A. J. M.; Gottschal, J. C.; Prins, R. A.Published in:Mycological ResearchIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1994Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Marvin-Sikkema, F. D., Driessen, A. J. M., Gottschal, J. C., & Prins, R. A. (1994). Metabolic EnergyGeneration In Hydrogenosomes Of The Anaerobic Fungus Neocallimastix - Evidence For A Functional-relationship With Mitochondria. Mycological Research, 98, 205-212.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.

Metabolic Energy Generation In Hydrogenosomes Of The Anaerobic Fungus Neocallimastix - Evidence For A Functional-relationship With Mitochondria

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Metabolic Energy Generation In Hydrogenosomes Of The Anaerobic Fungus Neocallimastix - Evidence For A Functional-relationship With Mitochondria

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