Bernard

Blaison

Branstetter

Campana

Chen

Cockerham

Compagno

Excoffier

Goudet

Heithaus

Holland

Kneebone

Librado

Lowe

Malausa

Meglecz

Meyer

Natanson

Papastamatiou

Pirog

Randall

Rooney

Rozen

Simpfendorfer

Van Oosterhout

PeerJ

The tiger sharkGaleocerdo cuvier(Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n= 101) from Reunion Island (South-Western Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni correction. Observed and expected heterozygosities ranged from 0.03 to 0.76 and from 0.03 to 0.77, respectively. No locus deviated from Hardy-Weinberg equilibrium and the globalFISof the population was of 0.04NS. Some of the eight loci developed here successfully cross-amplified in the bull sharkCarcharhinus leucas(one locus), the spinner sharkCarcharhinus brevipinna(four loci), the sandbar sharkCarcharhinus plumbeus(five loci) and the scalloped hammerhead sharkSphyrna lewini(two loci). We also designed primers to amplify and sequence a mitochondrial marker, the control region. We sequenced 862 bp and found a low genetic diversity, with four polymorphic sites, a haplotype diversity of 0.15 and a nucleotide diversity of 2 × 10−4.</jats:p

Agathe Pirog

Sébastien Jaquemet

Antonin Blaison

Marc Soria

Hélène Magalon

Crossref

Isolation and characterization of eight microsatellite loci fromGaleocerdo cuvier(tiger shark) and cross-amplification inCarcharhinus leucas, Carcharhinus brevipinna,Carcharhinus plumbeusandSphyrna lewini

Data S1: Dataset

Data S1: Dataset

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