The HNK-1 epitope is preferentially expressed on N-glycan at N413.

Abstract

<p>GluA2 (WT, N256S, N370S, N406S, or N413S) was transfected into HEK293 cells with HNK-1-synthesizing enzymes (GlcAT-P and HNK-1ST). After immunoprecipitation (IP) with anti-GluA2/3 polyclonal antibody, immunoprecipitates were immunoblotted with an HNK-1 monoclonal antibody and anti-GluA2/3 polyclonal antibody (A). The cell lysates were immunoblotted with an HNK-1 monoclonal antibody (Input) (B).</p

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