This article describes the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain

Adair, Tamarah

Afram, Patricia

Allen, Katherine G.

Archambault, Megan L.

Aziz, Rahat M.

Bagnasco, Filippa G.

Ball, Sarah

Barrett, Natalie A.

Benjamin, Robert C.

Blasi, Christopher J.

Bonilla, J. Alfred

Borst, Katherine

Braun, Mary A.

Broomell, Haley

Brown, Conner B.

Brynell, Zachary S.

Bue, Ashley B.

Burke, Sydney O.

Casazza, William

Cautela, Julia A.

Chen, Kevin

Chimalakonda, Nitish S.

Chudoff, Dylan

Connor, Jade A.

Cross, Trevor

Curtis, Kyra N.

Dahlke, Jessica A.

Deaton, Bethany M.

Degroote, Sarah J.

DeNigris, Danielle M.

DeRuff, Katherine C.

Dolan, Milan

Dunbar, David, 1953-

Egan, Marisa S.

Evans, Daniel R.

Fahnestock, Abby K.

Farooq, Amal

Finn, Garrett

Fratus, Christopher R.

Gaffney, Bobby L.

Garlena, Rebecca A.

Garrigan, Kelly E.

Gibbon, Bryan C.

Goedde, Michael A.

Guerrero Bustamante, Carlos A.

Harrison, Melinda

Hartwell, Megan

Hatfull, Graham F.

Heckman, Emily L.

Huang, Jennifer

Hughes, Lee E.

Hyduchak, Kathryn M.

Jacob, Aswathi E.

Jacobs-Sera, Deborah

Kaku, Machika

Karstens, Allen W.

Kenna, Margaret A.

Khetarpal, Susheel

King, Rodney A.

Klyczek, Karen

Kobokovich, Amanda L.

Kolev, Hannah

Konde, Sai A.

Kriese, Elizabeth

Lamey, Moran E.

Lantz, Carter N.

Lapin, Jonathan S.

Lawson, Temiloluwa O.

Lee, In Young

Lee, Scott M.

Lee-Soety, Julia Y.

Lehmann, Emily M.

London, Shawn C.

Lopez, A. Javier

Lynch, Kelly C.

Mageeney, Catherine M.

Martynyuk, Tetyana

Mathew, Kevin J.

Mavrich, Travis N.

McDaniel, Christopher M.

McDonald, Hannah

McManus, C. Joel

Medrano, Jessica E.

Mele, Francis E.

Menninger, Jennifer E.

Miller, Sierra N.

Minick, Josephine E.

Nabua, Courtney T.

Napoli, Carolina K.

Nkangabwa, Martha

Oates, Elizabeth A.

Ott, Cassandra T.

Pellerino, Sarah K.

Pinamont, William

Pirnie, Ross T.

Pizzorno, Marie C.

Plautz, Emilee J.

Pope, Welkin H.

Pruett, Katelyn M.

Rickstrew, Gabbi

PLoS ONE

The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45-68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate

Karen K Klyczek

J Alfred Bonilla

Deborah Jacobs-Sera

Tamarah L Adair

Patricia Afram

Katherine G Allen

Megan L Archambault

Rahat M Aziz

Filippa G Bagnasco

Sarah L Ball

Natalie A Barrett

Robert C Benjamin

Christopher J Blasi

Katherine Borst

Mary A Braun

Haley Broomell

Conner B Brown

Zachary S Brynell

Ashley B Bue

Sydney O Burke

William Casazza

Julia A Cautela

Kevin Chen

Nitish S Chimalakonda

Dylan Chudoff

Jade A Connor

Trevor S Cross

Kyra N Curtis

Jessica A Dahlke

Bethany M Deaton

Sarah J Degroote

Danielle M DeNigris

Katherine C DeRuff

Milan Dolan

David Dunbar

Marisa S Egan

Daniel R Evans

Abby K Fahnestock

Amal Farooq

Garrett Finn

Christopher R Fratus

Bobby L Gaffney

Rebecca A Garlena

Kelly E Garrigan

Bryan C Gibbon

Michael A Goedde

Carlos A Guerrero Bustamante

Melinda Harrison

Megan C Hartwell

Emily L Heckman

Jennifer Huang

Lee E Hughes

Kathryn M Hyduchak

Aswathi E Jacob

Machika Kaku

Allen W Karstens

Margaret A Kenna

Susheel Khetarpal

Rodney A King

Amanda L Kobokovich

Hannah Kolev

Sai A Konde

Elizabeth Kriese

Morgan E Lamey

Carter N Lantz

Jonathan S Lapin

Temiloluwa O Lawson

In Young Lee

Scott M Lee

Julia Y Lee-Soety

Emily M Lehmann

Shawn C London

A Javier Lopez

Kelly C Lynch

Catherine M Mageeney

Tetyana Martynyuk

Kevin J Mathew

Travis N Mavrich

Christopher M McDaniel

Hannah McDonald

C Joel McManus

Jessica E Medrano

Francis E Mele

Jennifer E Menninger

Sierra N Miller

Josephine E Minick

Courtney T Nabua

Caroline K Napoli

Martha Nkangabwa

Elizabeth A Oates

Cassandra T Ott

Sarah K Pellerino

William J Pinamont

Ross T Pirnie

Marie C Pizzorno

Emilee J Plautz

Welkin H Pope

Katelyn M Pruett

Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages.

Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

Abstract

Similar works

Full text

Available Versions

Directory of Open Access Journals

Crossref

UNT (University of North Texas) Digital Library