K Chandru

K Umamaheswari

N Rajendiran

R Baskar

S Chandirasekar

BMC Infectious Diseases

PubMed

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Antibacterial activity of gold nanoparticles and their toxicity assessment

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POSTER PRESENTATION Open Access
Antibacterial activity of gold nanoparticles and
their toxicity assessment
K Umamaheswari1*, R Baskar1, K Chandru3, N Rajendiran2, S Chandirasekar2
From 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)
Chennai, India. 30 January - 1 February 2014
Background
Several classes of antimicrobial nanoparticles (NPs) and
nanosized carriers for antibiotics delivery have proved
their effectiveness as alternative agents to combat the
antimicrobial resistance experienced with conventional
drugs. Gold nanostructures find extensive applications
in nano electronics and nanomedicine.
Methods
The present study involved the synthesis of gold nano-
particles using a sodium cholate, as both reducing and
capping agent and characterization by UV- Visible spec-
troscopy and High ResolutionTransmission Electron
Microscopy (HRTEM). The antibacterial activity on sig-
nificant bacterial species was evaluated by micro broth
dilution method. The mechanism for antibacterial action
of the gold nanoparticles were studied by reactive oxy-
gen species (ROS) generation that causes oxidative stress
to microbial cells and release of intracellular enzyme
lactate dehydrogenase into extracellular medium (LDH
assay) indicative of loss of cell membrane integrity.
Results
The synthesized gold nanoparticles showed characteristic
peak at 541 nm by UV- Visible spectroscopy with particle
size of 10nm as confirmed by HRTEM. The minimum
inhibitory concentration (MIC80) of the nanoparticles on
E. coli, S. typhi, P. aeruginosa, K. pneumoniae, ranged
from 20 to 40µg/mL. The ROS generation was directly
dependent on the concentration of the nanoparticles and
no detectable enzyme leakage was recorded.
Conclusion
The results showed appreciable antibacterial activity of
gold nanoparticles against the tested species and the possi-
ble mechanism of antibacterial activity may be due to
increased intracellular ROS generation causing oxidative
stress to the bacterial cells while LDH assay indicated that
nanoparticles caused no damage to the cell membrane
integrity.
Authors’ details
1Department of Biotechnology, University of Madras, Guindy, Chennai, India.
2Department of Polymer Science, University of Madras, Guindy, Chennai,
India. 3Centre for Biotechnology, Anna University, Guindy, Chennai, India.
Published: 27 May 2014
doi:10.1186/1471-2334-14-S3-P64
Cite this article as: Umamaheswari et al.: Antibacterial activity of gold
nanoparticles and their toxicity assessment. BMC Infectious Diseases 2014
14(Suppl 3):P64.
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* Correspondence: rk_uma@yahoo.com
1Department of Biotechnology, University of Madras, Guindy, Chennai, India
Full list of author information is available at the end of the article
Umamaheswari et al. BMC Infectious Diseases 2014, 14(Suppl 3):P64
http://www.biomedcentral.com/1471-2334/14/S3/P64
© 2014 Umamaheswari et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


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Antibacterial activity of gold nanoparticles and their toxicity assessment

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