A Bankevich

A Mentzer von

A Sjoling

AC Vicente

AM Almeida de

AR Quinlan

AR Wattam

C Lindenthal

CA Jacobi

D Hernandez

DA Rasko

DG Evans

DJ Ingle

EW Myers

F Canto Del

F Qadri

GB Lindblom

GP Munson

H Steinsland

HL DuPont

I Bolin

I Letunic

JM Fleckenstein

JS Farris

JW Sahl

K Hayashi

K Roy

K Schliep

KL Kotloff

LC Crossman

M So

MA Lasaro

MN Price

MS Donnenberg

P Kumar

Q Luo

R Lozano

RA Finkelstein

RA Nada

RB Sack

RC Edgar

RI Walker

S Schubert

SF Altschul

SK Patel

SM Turner

T Seemann

T Wirth

TH Hazen

W Gaastra

Scientific Reports

English

Sahl, Jason W

Sistrunk, Jeticia R

Baby, Nabilah Ibnat

Begum, Yasmin

Luo, Qingwei

Sheikh, Alaullah

Qadri, Firdausi

Fleckenstein, James M

Rasko, David A

Digital Commons@Becker

Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology

AbstractEnterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year in the developing world and are characterized on a molecular level by the presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) enterotoxins, as well as surface structures, known as colonization factors (CFs). Genome sequencing and comparative genomic analyses of 94 previously uncharacterized ETEC isolates demonstrated remarkable genomic diversity, with 28 distinct sequence types identified in three phylogenomic groups. Interestingly, there is a correlation between the genomic sequence type and virulence factor profiles based on prevalence of the isolate, suggesting that there is an optimal combination of genetic factors required for survival, virulence and transmission in the most successful clones. A large-scale BLAST score ratio (LS-BSR) analysis was further applied to identify ETEC-specific genomic regions when compared to non-ETEC genomes, as well as genes that are more associated with clinical presentations or other genotypic markers. Of the strains examined, 21 of 94 ETEC isolates lacked any previously identified CF. Homology searches with the structural subunits of known CFs identified 6 new putative CF variants. These studies provide a roadmap to exploit genomic analyses by directing investigations of pathogenesis, virulence regulation and vaccine development.</jats:p

Jason W. Sahl

Jeticia R. Sistrunk

Nabilah Ibnat Baby

Yasmin Begum

Qingwei Luo

Alaullah Sheikh

Firdausi Qadri

James M. Fleckenstein

David A. Rasko

Crossref

https://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=6962&amp;context=open_access_pubs

Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology

Abstract

Similar works

Full text

Available Versions

Digital Commons@Becker

Crossref