In vitro, actin filament tension correlates with the binding and apparent activity of the filament-severing protein cofilin, suggesting a molecular mechanism by which cells respond to changes in mechanical force

Andrianantoandro

Ashkin

Bamburg

Bershadsky

Chen

Colombo

Damien

De La Cruz

Egelman

Galkin

Gillespie

Hayakawa

Hitoshi Tatsumi

Huxley

Katoh

Kimihide Hayakawa

Kiyoshima

Mackay

Maniotis

Masahiro Sokabe

Matsushita

Matthews

McCullough

McGough

Medeiros

Michelot

Nagaoka

Obinata

Okreglak

Pavlov

Pellegrin

Perry

Pfaendtner

Prochniewicz

Sawada

Shimozawa

Spudich

Tamada

Tatsumi

Timmenga

Tsuda

Ueki

Wakabayashi

Wang

Yang

Yonezawa

English

PubMed

Intracellular and extracellular mechanical forces affect the structure and dynamics of the actin cytoskeleton. However, the underlying molecular and biophysical mechanisms, including how mechanical forces are sensed, are largely unknown. Actin-depolymerizing factor/cofilin proteins are actin-modulating proteins that are ubiquitously distributed in eukaryotes, and they are the most likely candidate as proteins to drive stress fiber disassembly in response to changes in tension in the fiber. In this study, we propose a novel hypothesis that tension in an actin filament prevents the filament from being severed by cofilin. To test this, we placed single actin filaments under tension using optical tweezers. When a fiber was tensed, it was severed after the application of cofilin with a significantly larger delay in comparison with control filaments suspended in solution. The binding rate of cofilin to an actin bundle decreased when the bundle was tensed. These results suggest that tension in an actin filament reduces the cofilin binding, resulting in a decrease in its effective severing activity.</jats:p

Crossref

The Journal of Cell Biology

Actin filaments function as a tension sensor by tension-dependent binding of cofilin to the filament

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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3257564

Actin filaments function as a tension sensor by tension-dependent binding of cofilin to the filament

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