The tail of yeast myosin II is localized to the division site by two distinct molecular pathways and sufficient for promoting actomyosin ring assembly, furrow ingression, and guidance in ECM remodeling

Ayscough

Balasubramanian

Beach

Bezanilla

Boyne

Carsten Wloka

Carvalho

Caviston

Christopher Dravis

Elizabeth A. Vallen

Erfei Bi

Evangelista

Guthrie

Gyuris

Huang

Huckaba

Izumikawa

Jianying Luo

Korinek

Lippincott

Lister

Longtine

Lord

Lupas

Masayuki Iwase

Mirko Travaglia

Mitchison

Mizuguchi

Moseley

Motegi

Naqvi

Nishihama

Pruyne

Puig

Rock

Ronen

Ross

Ryuichi Nishihama

Sabry

Sagot

Schmidt

Shannon

Straight

Strickland

Takaine

Tolliday

Trybus

Tully

Tyler

Vallen

VerPlank

Winter

Xiaodong Fang

Zang

English

PubMed

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with membrane trafficking during cytokinesis, remains poorly understood. Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its tail that act sequentially during the cell cycle. Before cytokinesis, Myo1 localization depends on the septin-binding protein Bni5. During cytokinesis, Myo1 localization depends on the IQGAP Iqg1. We also show that the Myo1 tail is sufficient for promoting the assembly of a “headless” AMR, which guides membrane deposition and extracellular matrix remodeling at the division site. Our study establishes a biphasic targeting mechanism for myosin II and highlights an underappreciated role of the AMR in cytokinesis beyond force generation.</jats:p

Crossref

The Journal of Cell Biology

Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

Cytokinesis in animal and fungal cells utilizes a contractile actomyosin ring (AMR). However, how myosin II is targeted to the division site and promotes AMR assembly, and how the AMR coordinates with membrane trafficking during cytokinesis, remains poorly understood. Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two distinct targeting signals in its tail that act sequentially during the cell cycle. Before cytokinesis, Myo1 localization depends on the septin-binding protein Bni5. During cytokinesis, Myo1 localization depends on the IQGAP Iqg1. We also show that the Myo1 tail is sufficient for promoting the assembly of a “headless” AMR, which guides membrane deposition and extracellular matrix remodeling at the division site. Our study establishes a biphasic targeting mechanism for myosin II and highlights an underappreciated role of the AMR in cytokinesis beyond force generation

Fang, X.

Luo, J. Y.

Nishihama, R.

Wloka, C.

Dravis, C.

Travaglia, M.

Iwase, M.

Vallen, Elizabeth Ann

Bi, E.

Works

Biphasic Targeting And Cleavage Furrow Ingression Directed By The Tail Of A Myosin II

Cytokinesis in budding yeast: the relationship between actomyosin ring function and septum formation.

For observation of Myo1-C-TAP, Myo1-TD2D-C-TAP, and MBP-myo1-Tail-TD2D structures by rotary-shadowing EM, 5–10.0 µl of each protein suspensions in 1x EM buffer was sprayed on freshly split mica

For observation of PS in different myo1 and iqg1 mutants by transmission EM, cells were fixed and processed as described previously (Nishihama et al.,

Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast.

High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.

Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis.

Rng2p, a protein required for cytokinesis in fission yeast, is a component of the actomyosin ring and the spindle pole body.

Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle.

Structural memory in the contractile ring makes the duration of cytokinesis independent of cell size.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3010076

Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

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