A Binder

A Casini

A Lampropoulos

A Sarrion-Perdigones

AC Groth

Arturo Casini

C Engler

CK Raymond

D Densmore

D Jovicevic

DE Cameron

DG Gibson

E Appleton

E Martínez-García

E Weber

G Linshiz

G Marsischky

G Pósfai

Geoffrey S. Baldwin

H Kim

HH Wang

J Bitinaite

J Kamens

J Quan

J-K Liu

JC Anderson

JD Keasling

JE Norville

JL Hartley

JP Torella

JS Dymond

KA Datsenko

KD Litcofsky

L Zhang

LJ Kahl

M Itaya

M Leguia

M Trubitsyna

Marko Storch

MJ Czar

MJ Lajoie

MJ Marcaida

MJ Smanski

MV Li

MZ Li

N Annaluru

NC Crook

NJ Hillson

P Guye

R Chao

R Silva-Rocha

RM Horton

RP Shetty

S Alberti

S Cardinale

S de Kok

S Kosuri

SC Sleight

SD Colloms

SN Cohen

T Cermak

T Ellis

T Sakuma

Tom Ellis

VK Mutalik

W-H Chen

WJ Blake

X Duportet

Y Sasaki

Y Zhang

Z Shao

Nature Reviews Molecular Cell Biology

Crossref

Bricks and blueprints: methods and standards for DNA assembly

DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods — including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) — have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts

Casini, A

Storch, M

Baldwin, GS

Ellis, T

Spiral - Imperial College Digital Repository

http://spiral.imperial.ac.uk/bitstream/10044/1/31281/2/Bricks%20Review%20Author%20Version.pdf

Bricks and blueprints: methods and standards for DNA assembly

Abstract

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Spiral - Imperial College Digital Repository