A Lundwall

A Shevchenko

A Yamaoka

AA Pragman

AM Canacci

AM Edstrom

AM Patel

Anne Pietinalho

Anne Puustinen

AQ Truong-Tran

B Magi

BL Mitchell

C Landi

C McSharry

CG Bornehag

CH Wolff

CM Greene

DC Straus

E Kriegova

EI Taskinen

EO Terho

ES Charlson

F Bourgeon

G Canesin

GB Pott

H Huang

H Klech

H Lilja

H Sano

H Wolff

Henrik Riska

Henrik Wolff

I Petrache

J Wu

J Xu

JE Carter

JK Stoller

JM Sallenave

K Karvala

KC Meyer

Kirsi Karvala

KL O’Connell

L Frol’ova

L Kohatsu

Laura Teirilä

LF Congote

LG Frigeri

LM Benson

LM Filkins

LS Newman

M Akpinar-Elci

M Girard

M Gomzi

M Hilty

M Jonsson

M Selman

MJ Mendell

Nades Palaniyar

NC Henderson

Niina Ahonen

P Kankkunen

P Rottoli

Päivi Piirilä

Päivi Tuominen

R Bruderer

R Dhami

R Wattiez

RA de Boer

RD Kornberg

RG Rodrigues

RI Zuberi

RL Jacobs

S Bao

S Hacker

S Holdenrieder

S Sato

SH Barondes

SH Randell

SL Farnworth

T Okamoto

T Sigsgaard

U Costabel

V Kumar

WJ Fisk

XN Ge

Y Cormier

Y Matsuda

Y Shiio

Y Zhang

PLoS ONE

PubMed

Crossref

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

<div>BackgroundHyperresponsiveness to inhaled non-infectious microbial particles (NIMPs) has been associated with illnesses in the airways. Hypersensitivity pneumonitis (HP) is considered to be the prototype for these NIMPs-related diseases; however, there is no consensus on the definitions or diagnostic criteria for HP and the spectrum of related illnesses.Methods and FindingsIn order to identify the possible diagnostic markers for illnesses associated with NIMPs in alveolar lining fluid, we performed a proteomic analysis using a two-dimensional difference gel electrophoresis on bronchoalveolar lavage (BAL) fluid from patients with exposure to NIMPs in the context of damp building-related illness (DBRI) or conditions on the borderline to acute HP, designated here as agricultural type of microbial exposure (AME). Samples from patients with HP and sarcoidosis (SARC) were included for reference. Results were compared to results of healthy subjects (CTR). Western blot was used for validation of potential marker proteins from BAL fluid and plasma. Protein expression patterns suggest a close similarity between AME and HP, while DBRI was similar to CTR. However, in DBRI the levels of the inflammation associated molecules galectin-3 and alpha-1-antitrypsin were increased. A novel finding emerging from this study was the increases of semenogelin levels in BAL fluid from patients with AME, HP and SARC. Histone 4 levels were increased in AME, HP and SARC. Elevated plasma levels of histone 2B were detected in HP and SARC, suggesting it to be a potential blood indicator for inflammatory diseases of the lungs.ConclusionsIn this study, the proteomic changes in bronchoalveolar lavage of DBRI patients were distinct from other NIMP exposure associated lung diseases, while changes in AME overlapped those observed for HP patient samples. Some of the proteins identified in this study, semenogelin and histone 4, could function as diagnostic markers for differential diagnosis between DBRI and HP-like conditions.</div

Laura Teirilä (600051)

Kirsi Karvala (600052)

Niina Ahonen (600053)

Henrik Riska (600054)

Anne Pietinalho (600055)

Päivi Tuominen (600056)

Päivi Piirilä (600057)

Anne Puustinen (600058)

Henrik Wolff (140722)

The Francis Crick Institute

Hyperresponsiveness to inhaled non-infectious microbial particles (NIMPs) has been associated with illnesses in the airways. Hypersensitivity pneumonitis (HP) is considered to be the prototype for these NIMPs-related diseases; however, there is no consensus on the definitions or diagnostic criteria for HP and the spectrum of related illnesses.In order to identify the possible diagnostic markers for illnesses associated with NIMPs in alveolar lining fluid, we performed a proteomic analysis using a two-dimensional difference gel electrophoresis on bronchoalveolar lavage (BAL) fluid from patients with exposure to NIMPs in the context of damp building-related illness (DBRI) or conditions on the borderline to acute HP, designated here as agricultural type of microbial exposure (AME). Samples from patients with HP and sarcoidosis (SARC) were included for reference. Results were compared to results of healthy subjects (CTR). Western blot was used for validation of potential marker proteins from BAL fluid and plasma. Protein expression patterns suggest a close similarity between AME and HP, while DBRI was similar to CTR. However, in DBRI the levels of the inflammation associated molecules galectin-3 and alpha-1-antitrypsin were increased. A novel finding emerging from this study was the increases of semenogelin levels in BAL fluid from patients with AME, HP and SARC. Histone 4 levels were increased in AME, HP and SARC. Elevated plasma levels of histone 2B were detected in HP and SARC, suggesting it to be a potential blood indicator for inflammatory diseases of the lungs.In this study, the proteomic changes in bronchoalveolar lavage of DBRI patients were distinct from other NIMP exposure associated lung diseases, while changes in AME overlapped those observed for HP patient samples. Some of the proteins identified in this study, semenogelin and histone 4, could function as diagnostic markers for differential diagnosis between DBRI and HP-like conditions

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Proteomic changes of alveolar lining fluid in illnesses associated with exposure to inhaled non-infectious microbial particles.

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

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