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药物胶囊中明胶动物来源分析方法的研究
Authors
刘涛
张贵锋
+4 more
王前
苏志国
马光辉
马润宇
Publication date
1 January 2008
Publisher
Abstract
研究在猪和牛胶原蛋白序列比对基础上,利用高效液相色谱质谱法(HPLC/MS)探索建立一种通过特征多肽分析药物胶囊中明胶动物来源的方法。实验利用三氯乙酸将胶囊溶液中的明胶进行了沉淀处理,使用胰蛋白酶将明胶进行酶解,使用HPLC/MS分析了八种不同产地胶囊酶解产物中的特征多肽。结果表明3种胶囊中只含有牛明胶,两种胶囊中只含有猪明胶,其余三种胶囊中检测出牛和猪明胶的特征多肽,表明样品中含有猪明胶和牛明胶。实验进一步考察明胶收集方法、酶解条件和羟基化修饰等对多肽识别的影响。结果表明HPLC/MS法检测酶解产物中的特征多肽是一种分析胶囊中明胶动物来源的有效方法
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Last time updated on 09/11/2016