ARAÚJO, W. L.

AZEVEDO, J. L.

DIAS, A.

FERREIRA, A.

LACAVA, P. T.

MELO, I. S. de

QUECINE, M. C.

SANTOS, V. C.

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Analysis and characterization of the bacteria community associated with tropical mangrove forest.

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Poster Session I - PS 9 - ENDOPHYTES AND PARASmC PLANTS PS 9-430 ANALYSIS AND CHARACTERIZATION OF THE BACTERIA COMMUNITY ASSOCIATED WITH TROP-ICAL MANGROVE FOREST Paulo Teixeira LACAVA', Armando DIAS', Vanessa Cicera SANTOS', Anderson FERREIRA'. Maria Carolina QUE-ClNE', WelingIDn Luiz ARAÚJO', llamarSoares MELO' and João Lúcio AZEVEDOl JDepartment ofGenetics, Hsco/a Superior de Agricultura "Luiz de Queiroz ", Universi/y oi São Paulo, ESALQ/USp, Piracicaba, Brazil; 2CenLro Nacional de Pesquisa de Monitoramento e . Avaliação de Impacto Ambienta( EMBRAPA, Jaguariúna, Brazi/. ptlacava@esalq.usp.br Mangrove ecosystems are rieh in organie matter, but they are nutnent-deficient ecosystems. especially of nltrogen and phos-phorus, which are indispensable for plant growth. Howcver~ microbial activity is responsible for major nutrient transfor-mations within a mangrove ecosystem and the mieroorgan-isms play importaot role in the nutrient recycling of tbe man-grove forest. The .aim of this work was to study the diversity of bacteria from rbizosphere and endophytes of mangrove by culture-dependent and denaturing gradient-gel electrophore-sis (DGGE). Also, the evaluation of enzyme production such as amylase, esterase, lipase, protease, pectinase and cellulose, by the baeteria isolated from mangrove was tested, as a bio-technological potential aspect. The predominant main groups of baeteria found from rhlzosphere were Vibrio, Microbacte-rium and Bacillus. The analyses by DGGE showed similarity of bacteria communities from rizhosphere groups and from endophytic groups. These results suggest that DGGE is a prac-ticable protocol to research the complex bacteria community of mangrove. The iso1ates presented enzymatic acrivity~ which was ranked as follows: proteolytic (69%) and amilolytic ac-tivity (56%), lipolytic (9%), esterasic (47%), pectinolytic (75%). However, cellulolytic activity was not detected. Fur-ther analyses of the dala collected in the present study are still being conducted and they will he presented at tIle congresso Financiai support: FAPESP, Biola Orant Program. PS9-431 ISOLATION AND MOLECULAR IDENTIFICATION OF ENDOPHYTIC FUNGI OF BENTONIC AND PHANE-ROGAM SEAWEEDOFMEDITERRANEAN SEA Ed~ ! ARRIDA TORNEL. M Víctoria RIERA RIERA, Maite V ÁZQUEZ LUIS, Pablo SÁNCHEZ JEREZ and Luis V. LOPEZ-LLORCA Multidisciplinary InstituIR for Environmental Studies (MIES) Ramón Margalef Department ofMari1W &iences anti Applied Biology, University of Alicante, 03080 Alicante, Spain. larriba.ed@gmail.com The tenn 'endophyte' defines organisms which are able to col-onize plants without causrng disease symptom. The srudy of marine endophytic fungi has many applications, such as search for possible biocontrol agents, and also for finding new and useful compounds for the pharmacelltica1 industry. Tbe objec-tive of the study was to isolate and identify fungaI endophytes associated with seaweed at the Mediterranean -coaS!. We iso-lated and identified 25 difTerent fungi. From tIlese, DNA was extracted and PCR amplification of the of the lTS 1 and ITS2 regions was followed by sequencing of this region. Based on the sequences a phylogeneti~ tree was constructed, in which 238 it was possible to dassify and identify most of the isolated fungi. This study was the first present a list of endophytes from seaweed, as well as obtaining 2 possibly ncw species. PS 9-432 EFFECI' ON ROOT PROTEIN EXPRESSION DUE TO ENDOPHYTIC COLONISATION OF BARLEY BY FUSARIUM EQUlSETl AND POCHONIA CHLAMYDO-SPORIA Jose G. MACIÁ-VICENTE, Hans-BÕfje JANSSON and Luis V. LOPEZ-LLORCA Multidisciplinary Institute for Environmental Studies (MIES) Ramón Margalef Department ofMarine &iences and Applied Biology, University of Alicante, 03080 Alicante, Spain. jgmv@ua.es fungai endophytes growing asymptomatically withín plant tis-sues can produce benefits for their host plants. such as growth enhancement and protection against biotic and abiotic stresses. EnOOphytic relationships form a dose interaction "between the fungas and rbe planto In thís work we have studied the root col-onisation of a model plant (barley) by different isolates of the fungi Fusarium equiseti and Pochonía chlamydosporia, and tbeir effect on the bost plant. These species have, in previous studies, shown an adequate endophytic capa~, and can reduce tbe se-verity and presence of fungai pathogens in the rhizosphere, and improvement of plant growt:h by P. chlamydosporia. The mecha-nisms involved in these phenomena are still not known. We have sttldied the differential protein expression patterns, using two-di-mensiona! elecrophoresis techniques. of barley roots inoculated or non-inoculated with the different isolates in an axenic system. Our preliminary results show similarities and specific differences (qualitative and quantitative) in protein spot pattems in root ex-tracts from the controls and four inoculated roots. These experi-ments will give us new knowledge on endophytic root coloniza-tion, and he important for the characterisation of the endophytes tested as possible biocontrol agents against root pathogens, as well as their plant growth promoting capacities. PS9-433 COLONIZATION OF MICROPROPAGATED SUGAR-CANE SEEDLlNGS BY ENDOPHYTlC BACTERIUM PANTOEAAGGWMERANS Maria Carolina QUEClNE', Priscilla Barros ROSSETTO', Paulo Teixeira LACAVA', Mateus MONDIN', Welington Luiz ARAÚJO', Aline Aparecida Pl7ZIRANI-K! ElNER'. lDepartment ifGenetics, Escola Superior de Agricultura ''Luiz de Queiroz ", Uniwrsi/y 01 São Paulo, ESALQ/USP, Piracicaba, Brazil aapldein@esalq.usp.br To srudy plan-interacting bacteria, such as endophytes, biocon-trol agents and rhizospheric bacteria require demonstration of reinfection and establishment by the inoculated str.ain in or on field-grown plants. Severa! biomarkers, such as green ftuores-cent protein (GFP), have become available to facililate localiza-tion and identification of bacteria in the infected plant tissues. In this context, the aim of this work was study tbe colonization of sugarcane by Pantoea agglomerans (33.1 strain)~ an importaot endophytic microorganism used on biocontrol and described as N2-fixing bacterium. For this study, we used two-plasmid sys-tem; the 33.1 strain was transformed with plasntid pNKBOR and same strain was transformed wirb other plasntid pNKGFP. Sug-arcane seedlings were inoculated using 33.1 :pNKBOR and 33.1: pNKGFP in a bacterial coucentration of 10' CFUlrnL. After 5, XIII Intemational Congress on Molecular Planl-Microbe Interactioll!; 

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Analysis and characterization of the bacteria community associated with tropical mangrove forest.

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