为了解实际养殖过程中使用阿维菌素药物后,鲈(lATEOlAbrAX JAPOnICuS)对阿维菌素的蓄积及消除规律,以指导阿维菌素药物在实际生产中的应用。以毒性试验为基础,在确定96 H半致死质量浓度(lC50)后,以0.25倍lC50,即指导用药量,质量浓度为4 ng·Ml-1,结合实际养殖用药情况,药浴72 H后换水,观察阿维菌素在鲈鱼肌肉组织中的残留与消除规律。文章建立高效液相色谱-串联质谱法测定鲈鱼中阿维菌素残留量的方法,并以此法测定鲈鱼肌肉组织中阿维菌素的质量浓度。结果表明,随着药浴时间的延长,鲈鱼肌肉组织中的阿维菌素质量分数逐步增加,在药浴结束时达到峰值8.767μg·kg-1,随后阿维菌素在体内的残留量随消除试验的进行逐渐下降,至第528小时降至检测限以下。To investigate the accumulation and elimination rules of avermectin in perch(Lateolabrax japonicus) muscle during actual breeding process,we determined the median lethal concentration in 96 h as 4 ng·mL-1 based on a toxicity test,and exposed the perch to 4 ng·mL-1 of avermectin for 72 h,and then transfered the solution into seawater.Thus,we develop a method by high performance liquid chromatography-tandem mass spectrometry to determine avermectin in perch muscle.The results show that the avermectin concentration in perch muscle gradually increases with passing medicated bath time,and reaches the peak of 8.767·kg-1 at the end of the medicated bath.Later,the avermectin in perch muscle gradually decreases and reduces below the detection limit at 528th hour.福建省海洋与渔业厅重点项目(KJXH-2010-007); 国家星火计划项目(2011GA72001
