合成生物素化普鲁兰多糖衍生物(BP),采用纳米沉淀法制备纳米颗粒(BPNs),考察制备条件对纳米颗粒性质影响,为进一步将其作为药物载体的研究提供基础。通过酯化反应将生物素羧基与普鲁兰多糖连接,生成的衍生物BP通过FI-IR和1H-NMR表征,取代度采用电感耦合等离子体光谱仪(ICP)确定;BPNs进行透射电镜、动态光散射仪和ζ电位仪表征与检测,颗粒表面生物素采用Quant*TagTMBiotin Kit生物素测定试剂盒测定。获得取代度21(BP1),46(BP2),81(BP3)3种衍生物,其中BP2和BP3能够制备纳米颗粒(LBPNs,HBPNs)。纳米颗粒呈球形,表面光滑规整,平均粒径100~300nm,水中ζ电位在-17mV左右。制备过程中,粒径随BP浓度(10~50mg/ml)增加而增加((136.2±46.8)nm,(190.8±89.6)nm和(254.5±140.0)nm)。调整水相组成为水:无水乙醇(v/v)1∶1和1∶2时粒径分别为(170.7±30.4)nm和(158.8±21.0)nm。HBPNs和LBPNs表面的生物素量为衍生物的(1.9±0.03)%和(2.0±0.04)%。生物素化...
【英文摘要】 To synthesize biotinylated pullulan derivation(BP) and prepare nanoparticles by nanoprecipitation.The effects of different parameters on the nanoparticles formation were investigated for further ions for the BP as cancer target drug carriers.Methods Biotin was covalently attached to pullulan by esterification.FI-IR spectroscopy(Bio-Rad Merlin) and 1H-NMR(INOVA 500Hz) were used to confirm the synthesis of the BP.The degree of BP substitution(DS) was determined by ICP.The physicochemical properties of the nan...国家重大科学研究计划资助项目(2006CB933300);; 国家教育部博士点基金资助项目(20060023050
