Construction of Tissue Engineered Dermal Substitutes and Its Effect on Wounds Repairing

Abstract

[中文文摘]以双层胶原-壳聚糖海绵为支架构建组织工程真皮替代物并研究其在大鼠创伤愈合中的作用.将双层胶原-壳聚糖海绵支架埋植于SD大鼠皮下以考察其组织相容性;将SD大鼠皮肤中分离的成纤维细胞种植在支架上体外构建人工真皮替代物,并将该替代物移植到SD大鼠皮肤缺损处以修复创面.皮下埋植实验显示支架植入早期有炎症反应,但一周后炎症反应逐渐减轻,而且有成纤维细胞和毛细血管的侵入.皮肤移植实验结果显示:术后第7天和第14天实验组创面表皮再生和肉芽组织增生比对照组更快更明显;第14天和第21天实验组创面面积明显小于对照组(p<0.05).因此以双层胶原-壳聚糖海绵为支架构建的组织工程真皮替代物具有良好的生物相容性,并能促进皮肤创面的愈合,可以作为人工真皮替代物用于皮肤缺损的修复。[英文文摘]A tissue engineered dermal substitute is developed to provide skin wounds healing of rats.The skin graft replacement has been designed as a dermal substitute comprising dermal fibroblast cells isolated from rat skin.The dermal substitute is constructed by a bilayer collagen-chitosan sponge scaffold,into which rat fibroblasts are seeded.Its tissue compatibility was studied by transplantation test on full-thickness wounds of SD rats.Subcutaneous implantation test shows early inflammatory response,which alleviated gradually af ter one week. The fibroblasts and capillary vessels grow into the porous sponge scaffold. Histological observations indicate that epidermis regeneration and acestoma hyperplasia of the experimental group are more obvious than that of the cont rol group on the 7th day and 14th day af ter t ransplantation. The healing rate of the experimental group is significantly higher than that of the cont rol group ( p < 0. 05) on the 14th day and 21st day. These experiment s provide evidence that the tissue engineered dermal substitute has good biocompatibility and promotes wounds healing. It could be a potential artificial dermal substitute to repair the skin defect.福建省科技计划项目(2003Y006)资