[中文文摘]目的:探讨尿激酶型纤维蛋白溶酶原激活剂相关蛋白(uPAR-associated protein，uPARAP/Endo180)、uP-AR和VEGF在鼻咽癌组织中的表达及临床意义。方法:采用免疫组化SP法检测58例鼻咽癌组织和30例慢性鼻咽炎组织中uPARAP、uPAR和VEGF蛋白的表达,分析其与鼻咽癌临床病理特征的关系。结果:鼻咽癌组织中uPARAP的阳性表达见于肿瘤间质的成纤维细胞和巨噬细胞,定位于胞膜和胞质,呈棕黄色颗粒状分布;uPAR阳性染色定位于肿瘤细胞与间质细胞胞浆内;VEGF阳性染色定位于肿瘤细胞胞浆内。鼻咽癌组织中uPARAP、uPAR和VEGF的阳性表达率分别为75.9%、81.0%和77.6%;与慢性鼻咽炎比较,uPARAP、uPAR和VEGF在鼻咽癌组织中的阳性表达率有显著性差异(P<0.05);鼻咽癌组织中uPARAP表达与uPAR、VEGF的表达水平呈明显正相关(P<0.05);uP-ARAP在角化型鳞癌、非角化型癌和未分化癌的阳性表达率分别为42.9%、66.7%和86.1%。随着肿瘤组织类型分化程度的下降,uPARAP表达水平有上升趋势(P<0.05)。不同组织学类型之间进行两两比较发现, uPARAP 的表达也有显著性差异( P<0.05) 。uPARAP 蛋白的表达与鼻咽癌组织病理分级、颈部淋巴结转移、临床分期密切相关( P<0.05),而与患者的年龄、性别等因素无关。结论: uPARAP、uPAR 和VEGF 在鼻咽癌组织中协同表达对促进鼻咽癌的扩散转移可能起重要作用, 联合检测这些指标有望成为判断鼻咽癌恶性程度和估计患者预后的重要生物学标志。[英文文摘]Objective: To investigate the expression and clinical significance of uPARAP/Endo180(uPAR-associated protein), urokinase plasminogen activator receptor(uPAR) and vascular endothelial growth factor(VEGF) in nasopharyngeal carcinoma(NPC). Methods: Streptavidin-horseradish peroxidase immunohistochemistry(SP-IHC) method was used to detect the expression of uPARAP, uPAR and VEGF in 58 samples of NPC and 30 samples of chronic nasopharynxitis. Results: The positive expression of uPARAP in NPC was mainly confined to mesenchymal cells, including fibroblasts and macrophages.
The uPARAP immunoreactivity was found as granulated diffuse cytoplasmic staining, as well as in a pattern suggesting localization
on the cell surface. uPAR in NPC tissues was evenly distributed in the cytoplasm of both cancer cells and mesenchymal cells. The expression of VEGF in NPC was mainly seen in the cytoplasm of tumor cells. In the NPC tissues, the expression rate of uPARAP, uPAR and VEGF was 75.9%, 81.0%, and 77.6%, respectively (P<0.05). A significant difference was found in the expression of uPARAP, uPAR and VEGF between NPC tissues and chronic nasopharynxitis tissues. The expression of uPARAP was positively correlated with the expression of uPAR and VEGF in NPC. The positive expression rate of uPARAP was 42.9% in keratinizing NPC, 66.7% in non- keratinizing NPC, and 86.1% in undifferentiated carcinoma.The expression of uPARAP was associated with the differentiation of NPC. The expression of uPARAP was significantly different among different pathological types of NPC(P<0.05). The expression of uPARAP in nasopharyngeal carcinoma was significantly correlated with pathological classification, tumor grade, cervical lymph node metastases and clinical.福建省自然科学基金资助(编号:C0610045
