Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90135/1/j.1600-0722.2011.00904.x.pd

Albertinazzi

Boyde

Castilho

Cerny

Chrostek

Couwenhoven

Cutroneo

DeMali

Filipenko

Fincham

Fukumoto

Gibson

Glogauer

Hart

Hatakeyama

Huang

Hunter

Jernvall

Krebsbach

Nishikawa

Noritake

Paine

Rajpar

Reith

Snead

Sugihara

Thesleff

Thomas

Vidali

Wright

European Journal Of Oral Sciences

The Ras-related C3 botulinum toxin substrate 1 (Rac1) gene encodes a 21-kDa GTP-binding protein belonging to the RAS superfamily. RAS members play important roles in controlling focal adhesion complex formation and cytoskeleton contraction, activities with consequences for cell growth, adhesion, migration, and differentiation. To examine the role(s) played by RAC1 protein in cell-matrix interactions and enamel matrix biomineralization, we used the Cre/loxP binary recombination system to characterize the expression of enamel matrix proteins and enamel formation in Rac1 knockout mice (Rac1(-/-)). Mating between mice bearing the floxed Rac1 allele and mice bearing a cytokeratin 14-Cre transgene generated mice in which Rac1 was absent from epithelial organs. Enamel of the Rac1 conditional knockout mouse was characterized by light microscopy, backscattered electron imaging in the scanning electron microscope, microcomputed tomography, and histochemistry. Enamel matrix protein expression was analyzed by western blotting. Major findings showed that the Tomes' processes of Rac1(-/-) ameloblasts lose contact with the forming enamel matrix in unerupted teeth, the amounts of amelogenin and ameloblastin are reduced in Rac1(-/-) ameloblasts, and after eruption, the enamel from Rac1(-/-) mice displays severe structural defects with a complete loss of enamel. These results support an essential role for RAC1 in the dental epithelium involving cell-matrix interactions and matrix biomineralization.This work was sponsored by the Canadian Institutes of Health Research

Huang, Z.

Kim, J.

Lacruz, R. S.

Bringas, P. Jr.

Glogauer, Michael

Bromage, T. G.

Kaartinen, V. M.

Snead, M. L.

TSpace (University of Toronto)

Epithelial-specific knockout of the Rac1 gene leads to enamel defects

TSpace Repository

Huang, Zhan

Kim, Jieun

Lacruz, Rodrigo S.

Bringas Jr, Pablo

Bromage, Timothy G.

Kaartinen, Vesa M.

Snead, Malcolm L.

Deep Blue Documents

Epithelial‐specific knockout of the  Rac1  gene leads to enamel defects

Zhan Huang

Jieun Kim

Rodrigo S. Lacruz

Pablo Bringas Jr

Michael Glogauer

Timothy G. Bromage

Vesa M. Kaartinen

Malcolm L. Snead

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https://deepblue.lib.umich.edu/bitstream/2027.42/90135/1/j.1600-0722.2011.00904.x.pdf

Epithelial‐specific knockout of the Rac1 gene leads to enamel defects

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