Molecular mechanism of the role of ROS signaling pathway in the antioxidant properties of TKE2 cells

目的:目前ROS在干细胞生物学中的作用还没有得到充分说明和了解。在过氧化氢（H2O2）诱导的氧化应激条件下，我们比较小鼠角膜上皮祖细胞（TKE2）[1]和成熟的小鼠角膜上皮细胞（MCE）的不同反应，重点研究是角膜干细胞的抗氧化细胞特性及相关机制。 方法:以小鼠角膜上皮祖细胞（TKE2）和成熟的小鼠角膜上皮细胞（MCE）为细胞模型，通过CCK8细胞活性实验检测不同浓度H2O2对小鼠角膜上皮的祖细胞（TKE2）和成熟的小鼠角膜上皮细胞（MCE）的影响。利用H2O2处理细胞24小时后，通过流式细胞术检测细胞内ROS的水平，利用免疫荧光染色检测细胞内氧化应激标志物3-硝基酪氨酸(3-NT)表达情况，...Purpose: The role of ROS in stem cell biology is not fully illustrated and understood. In this present research we compare the different responses under condition of oxidative stress induced by hydrogen peroxide (H2O2) between lately developed murine corneal epithelial progenitor cells (TKE2) and mature murine corneal epithelial cells (MCE) and investigate the antioxidant properties of TKE2 cells ...学位：理学硕士院系专业：医学院_药理学学号：2452013115355

The association between the antioxidant property and ROS system in murine corneal epithelial progenitor cells

目的：活性氧（ROS）是体内产生的一种高活性分子，低浓度可以调节细胞代谢，基因转录和磷酸酶的活性，高浓度则会导致氧化损伤。目前，有关干细胞自身特性的研究越来越受到学者们的重视，但既往文献中关于干细胞对氧化应激反应以及应激条件下细胞内相关酶活性的变化规律却很少报道。本课题聚焦于，在过氧化氢（H2O2）诱导的氧化应激条件下，活性氧生成关键因子NOX4和抗氧化酶核转录因子NRF2在小鼠角膜上皮祖细胞（TKE2）和成熟的小鼠角膜上皮细胞（MCE）中的不同作用及相关机制。 方法：利用小鼠角膜上皮祖细胞（TKE2）和成熟小鼠角膜上皮细胞（MCE）建立过氧化氢（H2O2）诱导的氧化应激细胞模型。首先我们通...Purpose: Reactive oxygen species (ROS) is a molecular of high activity produced in human body. It can regulate metabolism, gene transcription and phosphatase activity at low concentrations,while cause oxidative damage at higher concentrations.Nowadays,the stem cell propertities has increasingly aroused the public concern,but there are still few papers published about the the reponse and ...学位：医学硕士院系专业：医学院_药理学学号：2452013115356

Exploration of the anti-oxidant properties of human embryonic stem cells

目的:目前关于人胚胎干细胞（hESCs）对氧化应激反应以及细胞的抗氧化特性尚不完全清楚。本研究探索在氧化应激下在人胚胎干细胞（hESCs）和人角膜上皮细胞（HCE）的不同反应和作用及其机制研究，进一步阐述ESCs抗氧化应激的细胞特性及机制。 方法:利用H2O2和4-羟基壬烯醛（HNE）诱导的两种不同氧化应激模型重点观察、比较人胚胎干细胞（hESCs，选用H1细胞）和人成熟实体细胞:人角膜上皮细胞（HCE）的不同反应。通过CCK8细胞活性实验分别检测不同浓度的H2O2和HNE对H1、HCE以及人脐静脉内皮细胞（HUVECs）和人胃上皮细胞（GES-1）的影响；通过流式细胞术检测H1和HCE两种...Purpose: It is not fully elucidated about the cellular anti-oxidant properties of human embryonic stem cells（hESCs）.Here we investigated and compared the different responses against oxidative stress and the underlying mechanisms between human embryonic stem cells（hESCs）and human corneal epithelial cells（HCE）using two different oxidative stress models. Methods: We applied two oxidative stress ...学位：医学硕士院系专业：医学院_药理学学号：2452014115358

Effects of Chailing Jiangya Decoction on blood pressure, left ventricular hypertrophy and oxidative stress in spontaneously hypertensive rats

实验目的： 高血压病是常见的慢性疾病，中医药在改善高血压病症状及患者生活质量方面具有明显优势。经过多年的临床经验总结，钱林超教授认为，高血压病的主要病机之一是肝郁饮停，因此，临床上使用柴苓降压汤对此类高血压病患者进行治疗。此方疏肝利水，能调控血压、改善症状。本次实验通过研究柴苓降压汤对自发性高血压大鼠（SHR）血压、左心室及氧化应激的影响，明确其调控血压及左心室肥厚的机制，为柴苓降压汤的临床应用提供科学依据。 实验方法： 40只SHR随机分为模型组、替米沙坦组（7mg/kg）、柴苓降压汤高剂量组（57.6g/kg）、柴苓降压汤低剂量组（14.4g/kg），每组各10只；同时选择10只WK...OBJECTIVE: Hypertension is a common chronic disease. The traditional Chinese medicine has obvious advantages in improving the symptoms of hypertension and the quality of life. Prefessor Qian Linchao,after many years of clinical experience, has sunned up that one of the major pathogenesises of hypertension is stagnation of liver qi and excessive fluid. Therefore, we use Chailing Jiangya Decoction...学位：医学硕士院系专业：医学院_中医内科学学号：2452014115358

Experimental Study of the Abalone Shell on Cataract induced by Oxidative Stress

目的研究石决明提取物对体外培养的晶状体氧化应激性白内障形成的作用及机制。方法离体培养小鼠晶状体,应用不同浓度的石决明提取物预孵育晶状体24h后,加入1mm过氧化氢,继续培养3小时后恢复正常培养,72小时后观察小鼠晶状体混浊程度,测定晶状体组织培养液中的乳酸脱氢酶(LDH)含量,晶状体组织中还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力。结果石决明提取物在1~2 mg/ml浓度范围内减轻氧化应激造成的晶状体混浊,减少晶状体LDH的释放,提高组织内GSH含量和SOD活力。结论石决明提取物可减轻氧化应激白内障的形成,其作用主要与石决明提取物提高内源性抗氧化系统有关。Objective To study the effect of the abalone shell extract on oxidative stress induced cataract formation and its mechanism in cultured mouse lens in vitro. Methods The cultured mouse lens were pretreated with the abalone shell extract in different concentrations for24 hours,and then 1mm hydrogen peroxide was added and continued incubating for 3 hours,and they were changed to normal culture media.After 72 hours,the opacity of each lens was observed under an anatomical microscope,the content of lactate dehydrogenase( LDH)leakages,the content of the reduced glutathione( GSH) and activity of superoxide dismutase( SOD) in lens tissue were assayed. Results Abalone shell extract in the concentration range of 1 ~ 2 mg / ml reduced the lens opacity caused by oxidative stress,alleviated the release of LDH,and increased GSH content and SOD activity in cultured lens. Conclusion Abalone shell extract can alleviate the oxidative stress induced cataract formation,and this effect is mainly related to its improvement of the endogenous antioxidant system in lens.2012年福建省卫生厅中医药科研专项课题(No.WST201210);; 2013年福建省卫生厅中医药科研专项课题(No.wzhw201302);; 2014年厦门市科技局科技惠民项目课题(No.3502Z20144030

The effect of HIF-1α silencingon oxidative stress induced autophagy and apoptosis in renal carcinoma A498 cells

目的 探索肾癌A498细胞系在氧化应激状态下自噬发生的机制及其对细胞凋亡的影响，为探索治疗的新方法提供实验依据和理论基础。 方法 1. 应用不同浓度H2O2以及不同作用时间分别干预A498细胞，通过检测其CCK-8、ROS（活性氧簇）阳性细胞数量，确定构建A498细胞氧化应模型的H2O2最佳作用浓度及时间。选择200μM为H2O2处理细胞的最适浓度，分别作用A498细胞不同时间后，Westernblot检测氧化应激对自噬相关蛋白LC3Ⅱ和p62以及HIF-1α（低氧诱导因子1α）蛋白表达的变化。 2. 设计并合成一段针对人HIF-1α基因的shRNA，退火连接构建重组载体并酶切鉴定，将...Objective： To study the molecule mechanism of autophagy and apoptosis in renal carcinoma cells A498 cells under oxidative stress induced by hydrogen peroxide. The foundation was could be provided for new therapeutical methods of renal carcinoma. Methods: 1. A498 cells were treated with different concentration hydrogen peroxide (from 50μM to 400 μM) for different time (from 2 hous to 8 hours). ...学位：医学硕士院系专业：医学院_临床检验诊断学学号：2452014115352

Danggui sini Decoction attenuated diabetic neuropathy in diabetic rats

摘要 目的：糖尿病神经病变（Diabeticneuropathy，DN)是糖尿病常见的并发症之一。我们以往的研究发现当归四逆汤加减方(DangguisiniDecoction，DSD)能够缓解糖尿病神经病变引起的疼痛。本实验拟观察当归四逆汤加减方(DSD)对糖尿病大鼠坐骨神经和脊髓中一氧化氮（nitricoxide，NO）、一氧化氮合酶（Inducednitricoxidesynthase，NOS）、抗氧化酶、凋亡相关蛋白和硝基酪氨酸（nitrotyrosine，NT）蛋白和对后肢爪垫表皮内神经纤维影响。 方法：链脲佐菌素（streptozotocin，STZ）诱导SD大鼠1型糖尿病模...Abstract Objective—Diabetic neuropathy( DN ) is one of common complications of diabetes. Our previous studies found that Danggui Sini Decoction ( DSD ) can relieve the pain caused by diabetic neuropathy.To observe the effects of Danggui Sini Decoction ( DSD ) on hind legs of footpad intraepidermal nerve fiber, spinal cord and sciatic nerve nitric oxide( NO ) ,inducible nitric oxide synthase ( i...学位：医学硕士院系专业：医学院_内科学学号：2452013115346

NMR-based metabolomic analysis for mechanisms of both protections of HDL and apoA-I mimetic peptide D-4F, and injury of contrast media on endothelial cells

细胞代谢组学将高通量检测技术与多种数据分析方法结合，用于揭示细胞内整体、动态的代谢变化，在药物研究中发挥着重要的作用。本论文采用基于1H核磁共振（NMR）的细胞代谢组学方法，探讨与内皮细胞代谢相关的药物作用机制。 研究表明，载脂蛋白A-I（apoA-I）模拟肽D-4F展现出许多与高密度脂蛋白（HDL）相似的内皮保护功能。但是，目前并不清楚D-4F的内皮保护机制是否与HDL完全一样。因此，我们运用基于1HNMR的代谢组学方法，比较HDL和D-4F拮抗氧化型低密度脂蛋白（ox-LDL）诱导的内皮细胞紊乱的保护机制。我们发现ox-LDL不仅抑制内皮细胞的迁移，而且引发内皮细胞的代谢紊乱。这种代谢紊...Cell metabolomics is being used to reveal comprehensive and dynamic metabolic alterations in cells, which integrates high throughput detection technology and several data analysis methods. It plays a vital role in drug research. In this thesis, we conducted nuclear magnetic resonance (NMR)-based cell metabolomic analysis to elucidate the molecular mechanisms underlying the effects of drugs on endo...学位：理学硕士院系专业：化学化工学院_化学生物学学号：2052014115164

Study on the Function of the Neuron-Specific Protein TMEM59L in the Central Nervous System and in Oxidative Stress-Induced Cell Death

细胞凋亡是受基因严格调控的、自然发生的程序性细胞死亡过程，而细胞自噬是细胞组分自我降解的过程，二者在发育、组织稳态以及病原体防御中扮演着重要角色。细胞凋亡和细胞自噬发生异常会引发多种疾病包括癌症和神经系统疾病。神经元对于氧化应激十分敏感，并易于受到活性氧的攻击；当神经元长期暴露于包括过氧化氢在内的活性氧的攻击时会引发细胞死亡，导致各种神经系统疾病。因此，鉴定参与神经元死亡的新基因蛋白并揭示其作用机制，将有助于阐明神经系统疾病的致病机理。 TMEM59L为新发现的脑特异性高表达蛋白，其组织表达特异性预示着TMEM59L可能在神经系统中发挥重要作用。一项早期研究发现过表达TMEM59L可以引发细...Apoptosis is naturally occurring programmed cell death and autophagy is a process of self-degradation of cellular components,both of them are essential for normal development and tissue homeostasis. Dsregulation of apoptosis and autophagy can impair cellular functions and cause multiple diseases including cancers and neurological disorders. Neuronal cells are susceptible to oxidative insults. Chro...学位：理学博士院系专业：医学院_生理学学号：2452012015397

PM2.5在阿尔茨海默病中的研究进展

从PM2.5对阿尔茨海默病的标志物、炎症、脑的发育及其他因素造成的影响等方面入手,综合阐述了PM2.5与阿尔茨海默病之间的联系。PM2.5引发阿尔茨海默病的机制尚不明确,我们推测PM2.5导致的阿尔茨海默病的标志物增多、炎症反应及氧化损伤等可能是引发该病的重要原因。中国博士后科学基金面上资助项目(2016M600503