Determination of the degree of substitution (DS) of mixed cellulose esters by elemental analysis

Equations for transforming the results of elemental analysis (EA) into degree of substitution (DS) values for aliphatic cellulose esters were developed. The equations allow to determine the DS of cellulose derivatives bearing not only one but also two different acyl substituents. Error transmission studies revealed that the accuracy of the DS-values for a pure sample depends on both the number of carbon atoms in the substituents and on the uncertainty of the EA, especially from the hydrogen content. This method provides accurate DS-values (± 0.10 in most cases) if H-content determinations are within ± 0.1

Vacuolating cytotoxin (vacA) alleles of Helicobacter pylori comprise two geographically widespread types, m1 and m2, and have evolved through limited recombination

Vacuolating cytotoxin (vacA) alleles of Helicobacter pylori vary, particularly in their mid region (which may be type m1 or m2) and their signal peptide coding region (type s1 or s2). We investigated nucleotide diversity among vacA alleles in strains from several locales in Asia, South America, and the USA. Phylogenetic analysis of vacA mid region sequences from 18 strains validated the division into two main groups (m1 and m2) and showed further significant divisions within these groups. Informative site analysis demonstrated one example of recombination between m1 and m2 alleles, and several examples of recombination among alleles within these groups. Recombination was not sufficiently extensive to destroy phylogenetic structure entirely. Synonymous nucleotide substitution rates were markedly different between regions of vacA, suggesting different evolutionary divergence times and implying horizontal transfer of genetic elements within vacA. Non-synonymous/synonymous rate ratios were greater between m1 and m2 sequences than among m1 sequences, consistent with m1 and m2 alleles encoding functions fitting strains for slightly different ecological niches

Association between Helicobacter pylori genotypes and severity of chronic gastritis, peptic ulcer disease and gastric mucosal interleukin-8 levels: evidence from a study in the Middle East

Background: The varied clinical presentations of Helicobacter pylori (H. pylori) infection are most likely due to differences in the virulence of individual strains, which determines its ability to induce production of interleukin-8 (IL-8) in the gastric mucosa. The aim of this study was to examine association between cagA, vacA-s1 and vacA-s2 genotypes of H. pylori and severity of chronic gastritis and presence of peptic ulcer disease (PUD), and to correlate these with IL-8 levels in the gastric mucosa. Methods: Gastric mucosal biopsies were obtained from patients during esophagogastroduodenoscopy. The severity of chronic gastritis was documented using the updated Sydney system. H. pylori cagA and vacA genotypes were detected by PCR. The IL-8 levels in the gastric mucosa were measured by ELISA. Results: H. pylori cagA and/or vacA genotypes were detected in 99 patients (mean age 38.4±12.9; 72 males), of whom 52.5% were positive for cagA, 44.4% for vacA-s1 and 39.4% for vacA-s2; and 70.7% patients had PUD. The severity of inflammation in gastric mucosa was increased with vacA-s1 (p=0.017) and decreased with vacA-s2 (p=0.025), while cagA had no association. The degree of neutrophil activity was not associated with either cagA or vacA-s1, while vacA-s2 was significantly associated with decreased neutrophil activity (p=0.027). PUD was significantly increased in patients with cagA (p=0.002) and vacA-s1 (p=0.031), and decreased in those with vacA-s2 (p=0.011). The level of IL-8 was significantly increased in patients with cagA (p=0.011) and vacA-s1 (p=0.024), and lower with vacA-s2 (p=0.004). Higher levels of IL-8 were also found in patients with a more severe chronic inflammation (p=0.001), neutrophil activity (p=0.007) and those with PUD (p=0.001). Conclusions: Presence of vacA-s1 genotype of H. pylori is associated with more severe chronic inflammation and higher levels of IL-8 in the gastric mucosa, as well as higher frequency of PUD. Patients with vacA-s2 have less severe gastritis, lower levels of IL-8, and lower rates of PUD. The presence of cagA genotype is not associated with the severity of gastritis or IL-8 induction in the gastric mucosa. The association of cagA with PUD may be a reflection of its presence with vacA-s1 genotype

Helicobacter pylori Membrane Vesicles Stimulate Innate Pro- and Anti-Inflammatory Responses and Induce Apoptosis in Jurkat T Cells

Persistent Helicobacter pylori infection induces chronic inflammation in the human gastric mucosa, which is associated with development of peptic ulceration, gastric atrophy, and gastric adenocarcinoma. It has been postulated that secretion of immunomodulatory molecules by H. pylori facilitates bacterial persistence, and membrane vesicles (MV), which have the potential to cross the gastric epithelial barrier, may mediate delivery of these molecules to host immune cells. However, bacterial MV effects on human immune cells remain largely uncharacterized to date. In the present study, we investigated the immunomodulatory effects of H. pylori MV with and without the vacuolating cytotoxin, VacA, which inhibits human T cell activity. We show a high degree of variability in the toxin content of vesicles between two H. pylori strains (SS1 and 60190). Vesicles from the more toxigenic 60190 strain contain more VacA (s1i1 type) than vesicles from the SS1 strain (s2i2 VacA), but engineering the SS1 strain to produce s1i1 VacA did not increase the toxin content of its vesicles. Vesicles from all strains tested, including a 60190 isogenic mutant null for VacA, strongly induced interleukin-10 (IL-10) and IL-6 production by human peripheral blood mononuclear cells independently of the infection status of the donor. Finally, we show that H. pylori MV induce T cell apoptosis and that this is enhanced by, but not completely dependent on, the carriage of VacA. Together, these findings suggest a role for H. pylori MV in the stimulation of innate pro- and anti-inflammatory responses and in the suppression of T cell immunity

EQUITY SCHEMES IN SOUTH AFRICA: BENEFITS DELIVERED TO FARM WORKERS AS FUNCTION OF COMMERCIAL FARMERS' STRATEGY

With the end of Apartheid, the South African Government created and corrected agricultural factor and product market failures in its attempt to improve the distribution of wealth and incorporate previously disadvantaged farmers into the mainstream of agricultural activities. Some commercial farmers responded strategically to the new equity-based policies on factor and product markets by initiating equity-schemes with farm workers in order to maintain/increase their profits given the new local economic and political environment and global competition. Hence, most equity-schemes are organized according to the commercial farmers' capacities. The purpose of this paper is to analyze the organizational structure of the equity-schemes as a function of the commercial farmers strategy (production vs. marketing). This study is based on interviews of emerging farmers, former and present farm workers, commercial farmers, non-Governmental Organizations and Governmental agencies. The results show that equity-schemes tend to benefit commercial farmers but the degree to which farm workers' gain access to factor and product markets depends on commercial farmers' strategy. Then, the paper assesses alternative organizational structures of equity-schemes to maximize the benefits delivered to farm workers. Finally, utilizing the Peterson-Wysocki-Harsh Model, the issues of why the Government policies have not accomplished its objectives are discussed and recommendations to broaden the implementation of equity-schemes are suggested.Farm Management, Labor and Human Capital,

Desempenho produtivo de fêmeas das raças Canchim e Nelore. V. Desenvolvimento dos bezerros.

Foram estudados os pesos ao nascimento e a desmama, o ganho diário de peso do nascimento a desmama, as relações peso da vaca e idade da vaca ao parto e peso de bezerro a desmama por vaca em reprodução, de animais das raças Canchim e Nelore, filhos de vacas criadas juntas em regime exclusivo de pasto

Efeito da idade da vaca sobre intervalo de partos e fertilidade real de vacas da raça Nelore.

Este estudo foi realizado com intuito de se avaliar o efeito da idade da vaca sobre o intervalo de partos e fertilidade real de um rebanho de bovinos Nelore, pertencentes à Embrapa Cerrados, manejados em sistema extensivo de criação. Foram realizadas análises de variância para testar o efeito da idade da vaca ao parto, como covariável e análises de regressão a fim de se conhecer a evolução de cada característica em estudo. Observou-se efeito significativo da idade da vaca sobre todas as características analisadas. Houve decréscimo do intervalo de partos à medida que a vaca atingiu idade média de seis anos e após este período houve aumento do intervalo de partos. Em média, para estas características a idade ideal foi por volta dos sete anos de idade. A idade da vaca ao parto influenciou significativamete as características de intervalo de partos e fertilidade real. Portanto, há necessidade de ajustes, por meio de modelos de regressão, das características aos efeitos da idade da vaca como covariável, para que os animais, candidatos à seleção, tenham seus valores genéticos preditos com maior confiabilidade, garantindo assim maximização do ganho genético, por meio da seleção dos melhores reprodutores

Vaca pa’ la Vaca

Trabajo de síntesisEs una nueva alternativa de inversión en el sector Ganadero de Colombia, permite ser parte de una comunidad con excedentes de liquidez a partir de un monto mínimo de $150.000 pesos equivalente a una unidad, los cuales podrán ser negociados en nuestra plataforma.INTRODUCCIÓN E INFORMACIÓN GENERAL RESUMEN EJECUTIVO 1. DESCRIPCIÓN DEL PROYECTO DE INNOVACIÓN FINANCIERA CON BASE TECNOLÓGICA Y SOCIAL 2. OBJETIVOS DE VACA PA` LA VACA 3. INFORMACIÓN PRODUCTOS FINANCIEROS 4. ENCUESTA 5. ENTREVISTA 6. PLANEACIÓN ESTRATÉGICA 7. CONCEPTO DEL PRODUCTO, SERVICIO/MEJORA O FORTALECIMIENTO 8. LOGO VACA PA´ LA VACA 9. ESTUDIO DE MERCADO 10. FORMULACIÓN DEL MODELO DE NEGOCIO - PRODUCTO MÍNIMO VIABLE 11. ESTUDIO TÉCNICO 12. ESTUDIO ECONÓMICO Y FINANCIERO 13. ESTUDIO SOCIAL Y AMBIENTAL 14. NORMATIVIDAD 15. CONCLUSIONES 16. AGRADECIMIENTOS BIBLIOGRAFÍA ANEXOSEspecializaciónEspecialista en Formulación y Evaluación Social y Económica de Proyecto

Correlation of Helicobacter pylori genotypes with gastric histopathology in the central region of a South-European country

BACKGROUND: Outcome of Helicobacter pylori (H. pylori) infection results from interaction of multiple variables including host, environmental and bacterial-associated virulence factors. AIM: This study aimed to investigate the correlation of cagA, cagE, vacA, iceA and babA2 genotypes with gastric histopathology and disease phenotype in the central region of a South-European country. METHODS: This prospective study involved 148 infected patients (110 female; mean age 43.5 ± 13.4 years) submitted to endoscopy with corpus and antrum biopsies. H. pylori was cultured and DNA extracted from the isolates. Genotypes were determined by PCR. Histopathological features were graded according to the updated Sydney system and OLGA/OLGIM classification. Only patients with single H. pylori genotypes and complete histopathological results were included. RESULTS: Antrum samples presented higher degrees of atrophy, intestinal metaplasia, chronic inflammation and neutrophil activity. Genotype distribution was as follows: cagA-31.8 %; cagE-45.9 %; vacA s1a-24.3 %; vacA s1b-19.6 %; vacA s1c-0.7 %; vacA s2-55.4 %; vacA m1-20.9 %; vacA m2-79.1 %; vacA s1m1-18.9 %; vacA s1m2-25.7 %; vacA s2m1-2 %; vacA s2m2-53.4 %; iceA1-33.8 %; iceA2-66.2 %; babA2-12.2 %. CagA genotype was significantly associated with higher degrees of intestinal metaplasia, neutrophil activity, chronic inflammation and OLGIM stages. BabA2 was linked with higher H. pylori density. Strains with vacA s1m1 or vacA s1m1 + cagA positive genotypes had a significant association with peptic ulcer and vacA s2m2 with iron-deficient anemia. CONCLUSIONS: cagA, vacA s1m1 and babA2 genotypes are relatively rare in the central region of Portugal. cagA-positive strains are correlated with more severe histopathological modifications. This gene is commonly associated with vacA s1m1, and such isolates are frequently found in patients with peptic ulcer.info:eu-repo/semantics/publishedVersio