Environmental factors related to fungal infection and fumonisin accumulation during the development and drying of white maize kernels

In Southern Europe where whole maize kernels are ground and used for making bread and other food products, infection of the kernels by Fusarium verticillioides and subsequent fumonisin contamination pose a serious safety issue. The influence of environmental factors on this fungal infection and mycotoxin accumulation as the kernel develops has not been fully determined, especially in such food grade maize. The objectives of the present study were to determine which environmental factors may contribute to kernel invasion by F. verticillioides and fumonisin accumulation as kernels develop and dry in naturally infected white maize. Three maize hybrids were planted at two different sowing dates and kernel samples were collected 20, 40, 60, 80 and 100. days after silking. The percentage of kernels infected, and ergosterol and fumonisin contents were recorded for each sampling. F. verticillioides was the most prevalent species identified as the kernels developed. Temperature and moisture conditions during the first 80. days after silking favored natural kernel infection by F. verticillioides rather than by Aspergillus or Penicillium species. Fumonisin was found in kernels as early as 20. days after silking however significant fumonisin accumulation above levels acceptable in the EU did not occur until after physiological maturity of the kernel indicating that kernel drying in the field poses a high risk. Our results suggest that this could be due to increasing kernel damage by insects that favor fungal development, such as the damage by the moth Sitotroga cerealella, and to the occurrence of stress conditions for F. verticillioides growth that could trigger fumonisin biosynthesis, such as exposure to suboptimal temperatures for growth simultaneously with low water activity. © 2013 Elsevier B.V.MICINNSpanish Council of ResearchAutonomous Government of GaliciaEuropean Social FundThis research was supported by the National Plan for Research and Development of Spain (AGL2009-12770). A. Cao acknowledges funding from the JAE Program of the Spanish Council of Research. R. Santiago acknowledges postdoctoral contract “Isidro Parga Pondal” supported by the Autonomous Government of Galicia and the European Social Fund.Peer Reviewe

Effect of elevated atmospheric CO2 level on the abiotic and biotic stress tolerance of cereals

Abstract Effect of elevated CO2 level (EC) was studied on the tolerance of cereals to elevated temperature and drought and on the resistance of wheat to fungal diseases. In general, elevated growth temperature did not affect final grain size, thus having less harmful effects than heat stress or drought during grain-filling. The plants subjected to low water supply levels or elevated temperature had higher biomass and grain yield at EC than at the ambient level. Susceptible wheat varieties were, however, usually more prone to diseases when grown at EC, while resistant varieties remained resistant even at EC

Control of corn diseases in Illinois

"In cooperation with Office of Cereal Crops and Diseases, Bureau of Plant Industry, U.S. Department of Agriculture.

Indian Meal Moth Survivability in Stored Corn With Different Levels of Broken Kernels

Survivability of Indian meal moth, Plodia interpunctella (Hübner) (Lepi-doptera: Pyralidae), larvae fed a standard laboratory diet and whole corn with 0, 5 to 7, and 100% broken corn kernels, was assessed under laboratory conditions at 28o C, 65% relative humidity, and 14:10 h (L:D) photoperiod. A conventional yellow dent corn hybrid (about 3.9% oil content, dry basis) and a high-oil corn hybrid (about 7.7% oil content, dry basis) were tested. Survivability was measured as the percentage of pre-pupae, pupae, and adults observed at the end of the rearing period. For the standard laboratory diet, a mean of 97.5% larvae survived. Percentage of larval survival increased as the percentage of broken corn increased. Mean percentages of larval survival for the conventional yellow dent corn were 6.7, 63.8, and 80.0 for 0, 7, and 100% broken kernels, respectively. The mean percentages of larval survival for the high-oil corn hybrid were 28.3, 81.3, and 100.0 for 0, 5, and 100% broken kernels, respectively. Larval growth rate for high-oil corn was faster than for conventional corn. Results indicate that cleaning corn before storage could reduce P. interpunctella problems