2 research outputs found
Microcontroller-Based Sinusoidal Voltage Generation for Electrical Bio-Impedance Spectroscopy Applications
A sinusoidal voltage wave generator is proposed based on the use of micro-
processor digital signals with programmable duty-cycles, with application
to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in
cell cultures. The working principle relies on the time convolution of the programmed
microcontroller (μC) digital signals. The expected frequency is easily
tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks
to the μC clock frequency selection. This system has been simulated and
tested on the 8 bits μC Arduino™ Uno with ATmega328 version. Results obtained
prove that only three digital signals are required to fit the general specification
in ECIS experiments, below 1% THD accuracy, and show the appropriateness
of the system for the real-time monitoring of this type of biological
experiments.Spanish founded Project: TEC 2013- 46242-C3-1-P: Integrated Microsystem for Cell Culture AssaysFEDE
Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC ArduinoTM Uno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.This work was supported in part by the Spanish founded Project: TEC 2013- 46242-C3-1-P: Integrated Microsystem for Cell Culture Assays, co-financed with FEDER.Peer reviewe