1 research outputs found
In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments
The aim of our study is to evaluate different implant surface treatments using TiIV and
TiV in in vitro and in vivo studies. An in vitro study was established comprising four study groups
with treated and untreated TiIV titanium discs (TiIVT and TiIVNT) and treated and untreated TiV
titanium discs (TiVT and TiVNT). The surface treatment consisted in a grit blasting treatment with
alumina and double acid passivation to modify surface roughness. The surface chemical composition
and the surface microstructure of the samples were analyzed. The titanium discs were subjected
to cell cultures to determine cell adhesion and proliferation of osteoblasts on them. The in vivo
study was carried out on the tibia of three New Zealand rabbits in which 18 implants divided into
three experimental groups were placed (TiIVT, TiIVNT, and TiVT). Micro-computed tomography
(micro-CT) was performed to determine bone density around the implants. The results showed that
cell culture had minor adhesion and cell proliferation in TiIVT and TiVT within the first 6 and 24 h.
However, no differences were found after 48 h. No statistically significant differences were found in
the in vivo micro-CT and histological study; however, there was a positive trend in bone formation
in the groups with a treated surface. Conclusions: All groups showed a similar response to in vitro
cell proliferation cultures after 48 h. No statistically significant differences were found in the in vivo
micro-CT and histological study