2 research outputs found
Betaproteobacteria growth and nitrification rates during long-term natural dissolved organic matter decomposition experiments
As a first attempt to relate net nitrification rates with bacterial community structure in
the coastal embayment of the Ría de Vigo (NW Spain), a set of 6 long-term (lasting 53 to 74 d) dissolved
organic matter decomposition experiments were conducted in the dark and at a constant temperature
of 15°C with surface seawater collected in January, February, April, and June 2008. Net
nitrification rates were estimated from nitrate concentration changes. Evolution of bacterial community
composition was followed using catalyzed reporter deposition-fluorescent in situ hybridization
(CARD-FISH) and specific probes for 6 relevant bacterial groups, including Beta- and Gammaproteobacteria.
The growth rates of Betaproteobacteria showed a significant linear positive relationship
with nitrification rates that explained 82% of the observed variability, which strongly suggests that
the main nitrifying microorganisms during the incubations belonged to the Beta subclass of Proteobacteria.
A positive relationship was found between net nitrification rates and both ammonium
(r2 = 0.92) and phosphate (r2 = 0.97) concentrations, which suggests a tight link among the nitrogen
and phosphorus cycles, likely as a result of the role of phosphate as the bacterial growth-limiting
nutrient.Peer reviewe
Betaproteobacteria growth and nitrification rates during long-term natural dissolved organic matter decomposition experiments
As a first attempt to relate net nitrification rates with bacterial community structure in
the coastal embayment of the Ría de Vigo (NW Spain), a set of 6 long-term (lasting 53 to 74 d) dissolved
organic matter decomposition experiments were conducted in the dark and at a constant temperature
of 15°C with surface seawater collected in January, February, April, and June 2008. Net
nitrification rates were estimated from nitrate concentration changes. Evolution of bacterial community
composition was followed using catalyzed reporter deposition-fluorescent in situ hybridization
(CARD-FISH) and specific probes for 6 relevant bacterial groups, including Beta- and Gammaproteobacteria.
The growth rates of Betaproteobacteria showed a significant linear positive relationship
with nitrification rates that explained 82% of the observed variability, which strongly suggests that
the main nitrifying microorganisms during the incubations belonged to the Beta subclass of Proteobacteria.
A positive relationship was found between net nitrification rates and both ammonium
(r2 = 0.92) and phosphate (r2 = 0.97) concentrations, which suggests a tight link among the nitrogen
and phosphorus cycles, likely as a result of the role of phosphate as the bacterial growth-limiting
nutrient.Peer reviewe